Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,466,196	A→G	pseudogene (805/2518 nt)	ydbA →	putative outer membrane protein, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,196	0	A	G	100.0%	200.4 / NA	57	pseudogene (805/2518 nt)	ydbA	putative outer membrane protein, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/34);  total (23/34)

AACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACC  >  NC_000913/1466073‑1466330                                                                                                                            |                                                                                                                                       aacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGAc                                                                                                                        <  2:178182/140‑1 (MQ=255) aacaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGAc                                                                                                                        <  2:975682/140‑1 (MQ=255)  acaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACg                                                                                                                       <  2:628266/140‑1 (MQ=255)  acaaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACg                                                                                                                       <  2:185861/140‑1 (MQ=255)    aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGa                                                                                                                         >  1:247473/1‑136 (MQ=255)    aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGa                                                                                                                         <  2:247473/136‑1 (MQ=255)    aaTGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGt                                                                                                                     >  1:352112/1‑140 (MQ=255)      tGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTgg                                                                                                                   >  2:234849/1‑140 (MQ=255)        gCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGAt                                                                                                                 <  1:305934/140‑1 (MQ=255)             accaccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACTGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGAc                                                                                                                        >  2:850801/1‑128 (MQ=255)             accaccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACTGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGAc                                                                                                                        <  1:850801/128‑1 (MQ=255)              ccaccGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAg                                                                                                           <  1:678177/140‑1 (MQ=255)                    ttGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCAcc                                                                                                     <  1:72049/140‑1 (MQ=255)                          gTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGAcc                                                                                               <  2:209373/140‑1 (MQ=255)                                 ttCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCg                                                                                        >  2:686516/1‑140 (MQ=255)                                      ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                   <  1:516570/140‑1 (MQ=255)                                      ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                   <  1:828927/140‑1 (MQ=255)                                      ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                   <  1:789707/140‑1 (MQ=255)                                      ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                   <  2:651401/140‑1 (MQ=255)                                      ccGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCg                                                                                   <  1:459320/140‑1 (MQ=255)                                        ggTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGa                                                                                 <  1:849398/140‑1 (MQ=255)                                         gTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                >  2:972737/1‑140 (MQ=255)                                           aCGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTc                                                                              <  1:234849/140‑1 (MQ=255)                                                aaTCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATgg                                                                         <  1:47721/140‑1 (MQ=255)                                                 aTCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATggg                                                                        >  1:617727/1‑140 (MQ=255)                                                    aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAt                                                                           >  2:603291/1‑134 (MQ=255)                                                    aaCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCAt                                                                           <  1:603291/134‑1 (MQ=255)                                                         taataaCGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGa                                                                >  2:565839/1‑140 (MQ=255)                                                               cGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACg                                                          <  1:591303/140‑1 (MQ=255)                                                               cGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACg                                                          <  2:228979/140‑1 (MQ=255)                                                               cGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACg                                                          <  2:232976/140‑1 (MQ=255)                                                                gggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACgg                                                         >  2:49887/1‑140 (MQ=255)                                                                 ggAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGt                                                        >  1:209937/1‑140 (MQ=255)                                                                  gAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTg                                                       >  2:93411/1‑140 (MQ=255)                                                                       tGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAg                                                  >  1:596997/1‑140 (MQ=255)                                                                            cAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCAt                                             <  1:972737/140‑1 (MQ=255)                                                                             aGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGAACCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATc                                            <  2:663142/140‑1 (MQ=255)                                                                               gACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGt                                          >  1:13717/1‑140 (MQ=255)                                                                                 cGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACg                                                          >  1:344852/1‑122 (MQ=255)                                                                                 cGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACg                                                          <  2:344852/122‑1 (MQ=255)                                                                                  ggCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCaa                                       >  2:767103/1‑140 (MQ=255)                                                                                   gCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAAt                                      >  2:37131/1‑140 (MQ=255)                                                                                    cGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATa                                     >  1:464405/1‑140 (MQ=255)                                                                                         tGGATGTCAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGaa                                >  2:193522/1‑140 (MQ=255)                                                                                                cAGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCgaga                         <  2:352112/140‑1 (MQ=255)                                                                                                 aGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGagag                        <  1:193425/140‑1 (MQ=255)                                                                                                 aGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGagag                        >  2:547482/1‑140 (MQ=255)                                                                                                 aGCGGCGGCGGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGagag                        >  1:569860/1‑140 (MQ=255)                                                                                                  gcggcggcgGTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGc                       <  1:79634/140‑1 (MQ=255)                                                                                                     gcggcggTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCAcc                    <  2:464405/140‑1 (MQ=255)                                                                                                         cggTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCa                <  1:547482/140‑1 (MQ=255)                                                                                                           gTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCAcc              <  2:569860/140‑1 (MQ=255)                                                                                                           gTCACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCAcc              <  1:37131/140‑1 (MQ=255)                                                                                                             cACGGTATTGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCaa            >  2:918480/1‑140 (MQ=255)                                                                                                                     tGATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTggca    >  2:173190/1‑140 (MQ=255)                                                                                                                      gATATCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTggcac   <  2:617727/140‑1 (MQ=255)                                                                                                                       atatCGCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTggcacc  <  2:209937/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                       AACAATGGCAAAACCACCGTTGACGGTAAAGATTCCACCGGTACGGAAATCAACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACC  >  NC_000913/1466073‑1466330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑