Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,664	G→A	G1202S (GGC→AGC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,664	0	G	A	100.0%	130.5 / NA	38	G1202S (GGC→AGC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/14);  total (24/14)

AGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAA  >  NC_000913/1486530‑1486798                                                                                                                                       |                                                                                                                                       aGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTc                                                                                                                                   >  1:612402/1‑140 (MQ=255)    aaaTCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAAATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCaaa                                                                                                                                >  2:561805/1‑140 (MQ=255)         cTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCt                                                                                                                           <  1:129275/140‑1 (MQ=255)                cAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGac                                                                                                                    <  2:488717/140‑1 (MQ=255)                        aaTATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCg                                                                                                            >  2:553248/1‑140 (MQ=255)                            tCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGatat                                                                                                        <  2:47770/140‑1 (MQ=255)                             caacaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATAtt                                                                                                       >  1:629607/1‑140 (MQ=255)                               acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:236223/1‑140 (MQ=255)                               acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:633722/1‑140 (MQ=255)                               acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:152671/1‑140 (MQ=255)                                 aaaCGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCa                                                                                                   <  2:849630/140‑1 (MQ=255)                                               gCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAAc                                                                                     >  1:553648/1‑140 (MQ=255)                                                        tatGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGaaa                                                                            >  2:73974/1‑140 (MQ=255)                                                             ccATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTGACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTg                                                                       >  2:684815/1‑140 (MQ=255)                                                                tGGCGCGGGGGCGGGATGACAGAAAAGCGAAGAGGGACAGGGTGGAATATCTCGGGTGGGACACGGGTAACAGCTTGAAAAGTCGGGGCTACACGCTGCGATATTTGCAGGCGAGTGAAAAACGACGGGAAAAACTGGCg                                                                    >  2:849348/1‑140 (MQ=255)                                                                 ggCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCgg                                                                   <  2:868136/140‑1 (MQ=255)                                                                 ggCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCgg                                                                   <  2:443150/140‑1 (MQ=255)                                                                     cTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGa                                                               >  1:493938/1‑140 (MQ=255)                                                                       ggggCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             <  1:553248/140‑1 (MQ=255)                                                                             ttCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             >  1:929476/1‑134 (MQ=255)                                                                             ttCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             <  2:929476/134‑1 (MQ=255)                                                                              tCTGACATTAAAGCGCAAATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATcg                                                      <  1:561805/140‑1 (MQ=255)                                                                                    aTTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTATCAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCg                                                <  1:605703/140‑1 (MQ=255)                                                                                        aaGCGCAGATGGGCGGGTTGGTATGTCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTgcg                                            >  1:944239/1‑140 (MQ=255)                                                                                                aTGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCt                                    >  2:859587/1‑140 (MQ=255)                                                                                                           ggTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGaaa                         <  1:73974/140‑1 (MQ=255)                                                                                                             tataTCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGaaaaa                       >  1:189403/1‑139 (MQ=255)                                                                                                                  cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCca                  >  1:663111/1‑140 (MQ=255)                                                                                                                  cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAATCGAAAACGTccc                  >  2:864682/1‑139 (MQ=255)                                                                                                                  cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGACGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCAGATCGCGACCCTGCACAGATGCTGAAAGTCGAAAATGTccc                  >  2:871149/1‑139 (MQ=255)                                                                                                                   gcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcag                 >  1:509419/1‑140 (MQ=255)                                                                                                                      gTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcagcag              >  2:226279/1‑140 (MQ=255)                                                                                                                      gTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcagcag              >  1:792021/1‑140 (MQ=255)                                                                                                                       ttttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAgg             >  2:207207/1‑140 (MQ=255)                                                                                                                       ttttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTG‑AAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGc            >  1:365308/1‑140 (MQ=255)                                                                                                                        tttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGc            <  1:849348/140‑1 (MQ=255)                                                                                                                          tGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGt          <  2:553648/140‑1 (MQ=255)                                                                                                                              aCTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGGTGGTTCCCAACCCGACCGGGGGGAGATGGTGGAAGGTGGAAAAGTCCAGCAGGCgggggg      >  2:839178/1‑138 (MQ=255)                                                                                                                                  gTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCaa  <  1:839178/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAA  >  NC_000913/1486530‑1486798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑