Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,554,493	T→C	T393A (ACC→GCC)	maeA ←	malate dehydrogenase, NAD‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,554,493	0	T	C	100.0%	99.9 / NA	29	T393A (ACC→GCC)	maeA	malate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/15);  total (14/15)

TCGGTCCAGGCGATAATGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGGCAGCAGG  >  NC_000913/1554366‑1554629                                                                                                                                |                                                                                                                                         tCGGTCCAGGCGATAATGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGAc                                                                                                                              <  1:832555/140‑1 (MQ=255)  cGGTCCAGGCGATAATGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACg                                                                                                                             <  1:60602/140‑1 (MQ=255)        aGGCGATAATGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATc                                                                                                                       >  2:431541/1‑140 (MQ=255)               aaTGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAatat                                                                                                                >  1:1863/1‑140 (MQ=255)                aTGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATc                                                                                                               <  1:398504/140‑1 (MQ=255)                aTGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATc                                                                                                               <  1:434458/140‑1 (MQ=255)                aTGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATc                                                                                                               <  2:848328/140‑1 (MQ=255)                  gTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTg                                                                                                             <  1:23128/140‑1 (MQ=255)                          gtgtGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGa                                                                                                                    <  2:94035/125‑1 (MQ=255)                          gtgtGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGa                                                                                                                    >  1:94035/1‑125 (MQ=255)                                   ccACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGcacc                                                                                            <  2:729102/140‑1 (MQ=255)                                                               cATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGt                                                                <  1:431541/140‑1 (MQ=255)                                                                 tCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCg                                                              <  2:162762/140‑1 (MQ=255)                                                                          gACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAg                                                     <  2:1863/140‑1 (MQ=255)                                                                               ggACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCg                                                              <  1:425009/126‑1 (MQ=255)                                                                               ggACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCg                                                              >  2:425009/1‑126 (MQ=255)                                                                                 aCAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGa                                              >  1:50015/1‑140 (MQ=255)                                                                                                         gatCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGtt                      <  2:513931/140‑1 (MQ=255)                                                                                                          atCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCgcgc                                   >  2:165726/1‑126 (MQ=255)                                                                                                          atCTCTTCCGTAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCgcgc                                   <  1:165726/126‑1 (MQ=255)                                                                                                                    tAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGaaa           >  2:963317/1‑140 (MQ=255)                                                                                                                    tAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGa             >  2:59203/1‑138 (MQ=255)                                                                                                                    tAAACAGCCCGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGa             <  1:59203/138‑1 (MQ=255)                                                                                                                           cccGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGgcagca    >  2:426330/1‑140 (MQ=255)                                                                                                                           cccGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGgcagca    >  2:219803/1‑140 (MQ=255)                                                                                                                           cccGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGgcagca    >  2:628036/1‑140 (MQ=255)                                                                                                                            ccGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGGcagcag   >  1:88975/1‑140 (MQ=255)                                                                                                                            ccGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGA‑GTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGGCAGCAgg  >  1:439962/1‑140 (MQ=255)                                                                                                                             cGGCCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGGCAGCAgg  >  1:948296/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                         TCGGTCCAGGCGATAATGTCCTGCGGTGTGGCTTCCACGCGTGACGTCGGGTTAGACAGCGGCATCACGATCGGACGCGGACAGTGTTTATGCATCTCACGGATGATCTCTTCCGTAAACAGCCCGGTCTGTCCTGAGACGCCAATCAGAATATCTGGTTTTACATTGCGCACCACATCCAGCAGTGACAGCACATCGCTGTCGGTATCCCAGTCACTGAGGTTTTCGCGCTTCTGCACCAGTTTGGTCTGGAAAGGCAGCAGG  >  NC_000913/1554366‑1554629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑