Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,663,793	T→C	V62A (GTC→GCC)	ynfH →	putative menaquinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,663,793	0	T	C	100.0%	174.3 / NA	50	V62A (GTC→GCC)	ynfH	putative menaquinol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/25);  total (25/25)

TCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTC  >  NC_000913/1663659‑1663923                                                                                                                                       |                                                                                                                                   tCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCa                                                                                                                                  >  1:293244/1‑137 (MQ=255)    aTGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCAcc                                                                                                                            >  2:538261/1‑140 (MQ=255)    aTGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCAcc                                                                                                                            >  2:520722/1‑140 (MQ=255)    aTGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCAcc                                                                                                                            >  2:187828/1‑140 (MQ=255)     tGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCt                                                                                                                           >  2:618900/1‑140 (MQ=255)     tGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCt                                                                                                                           <  1:618900/140‑1 (MQ=255)         tagtagGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGa                                                                                                                       >  1:613294/1‑140 (MQ=255)             agGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGc                                                                                                                   >  1:252956/1‑140 (MQ=255)               gcgcgaTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCa                                                                                                                 >  1:11481/1‑140 (MQ=255)                 gcgcTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACt                                                                                                               >  1:881540/1‑140 (MQ=255)                    cTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCg                                                                                                            >  1:80311/1‑140 (MQ=255)                     tGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCgg                                                                                                           <  2:613294/140‑1 (MQ=255)                      gATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCggg                                                                                                          >  1:785992/1‑140 (MQ=255)                               cGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACt                                                                                                 >  2:179370/1‑140 (MQ=255)                                      ggCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTgaa                                                                                          <  2:925385/140‑1 (MQ=255)                                           gTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTgaatcgaa                                                                                     <  2:252956/140‑1 (MQ=255)                                            ttCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTgaatcgaat                                                                                    >  2:342633/1‑140 (MQ=255)                                               gccgccAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCgg                                                                                 <  2:792507/140‑1 (MQ=255)                                               gccgccAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCgg                                                                                 <  1:28280/140‑1 (MQ=255)                                               gccgccAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCgg                                                                                 <  1:538123/140‑1 (MQ=255)                                                    cAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCtt                                                                            <  2:881540/140‑1 (MQ=255)                                                    cAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCtt                                                                            <  1:686775/140‑1 (MQ=255)                                                      aaaaTGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCt                                                                          <  1:179370/140‑1 (MQ=255)                                                         aTGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGt                                                                       <  1:187828/140‑1 (MQ=255)                                                          tGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTc                                                                      <  1:226089/140‑1 (MQ=255)                                                           gACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCt                                                                     <  1:538261/140‑1 (MQ=255)                                                                             aTTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCTCCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATtgctgc                                                   <  1:87783/140‑1 (MQ=255)                                                                               tGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGG‑TTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCgg                                                <  2:434618/140‑1 (MQ=255)                                                                                gTCCGTGGCATGTTTTTCCTCTGACTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCgg                                                <  1:641209/140‑1 (MQ=255)                                                                                      ggCATGTTTTTTCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCat                                          >  1:491944/1‑140 (MQ=255)                                                                                      ggCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATtgctgc                                                   >  1:189220/1‑131 (MQ=255)                                                                                      ggCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATtgctgc                                                   <  2:189220/131‑1 (MQ=255)                                                                                      ggCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCat                                          >  1:14022/1‑140 (MQ=255)                                                                                      ggCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCat                                          <  2:226650/140‑1 (MQ=255)                                                                                       gCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCata                                         >  2:838345/1‑140 (MQ=255)                                                                                                     tGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTgg                           <  2:785992/140‑1 (MQ=255)                                                                                                     tGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTCCCATGCACCTGGGATCGCCACTGCGGGAGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTgg                           >  2:245427/1‑140 (MQ=255)                                                                                                      ggCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCg                               <  1:604023/135‑1 (MQ=255)                                                                                                      ggCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGc                          >  2:398551/1‑140 (MQ=255)                                                                                                      ggCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGc                          <  1:342633/140‑1 (MQ=255)                                                                                                      ggCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATAGTTTTTGCCg                               >  2:604023/1‑135 (MQ=255)                                                                                                       gCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCg                         >  1:682426/1‑140 (MQ=255)                                                                                                       gCTGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCg                         >  2:693410/1‑140 (MQ=255)                                                                                                         tGTTGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGa                       <  2:11481/140‑1 (MQ=255)                                                                                                            tGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGa                       <  1:606680/137‑1 (MQ=255)                                                                                                            tGATGGGCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGATGGCGGa                       >  2:606680/1‑137 (MQ=255)                                                                                                                gggCGTCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTAtggt                >  2:389920/1‑140 (MQ=255)                                                                                                                     tCGGGTTTATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTAtggtggctg           >  1:601511/1‑140 (MQ=255)                                                                                                                            tATTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAg    >  1:193534/1‑140 (MQ=255)                                                                                                                             aTTGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGt   <  2:491944/140‑1 (MQ=255)                                                                                                                              ttGCCTCTGCCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTc  <  2:601511/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   TCAATGCGTAGTAGGCGCGCTGATTGTTAGCGGAATTGGCTGGTTCGCCGCCAAAAATGACGCAGACCGTCAGCGTATTGTCCGTGGCATGTTTTTCCTCTGGCTGTTGATGGGCGTCGGGTTTATTGCCTCTGTCATGCACCTGGGATCGCCACTGCGGGCGTTTAACTCCCTGAATCGAATCGGAGCTTCTGGTCTGAGTAACGAGATTGCTGCCGGTTCCATATTTTTTGCCGTTGGCGGATTATGGTGGCTGGTGGCAGTC  >  NC_000913/1663659‑1663923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑