Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,665,769	C→G	A152G (GCC→GGC)	clcB →	putative chloride:H(+) antiporter ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,665,769	0	C	G	100.0%	115.1 / NA	34	A152G (GCC→GGC)	clcB	putative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (15/19);  total (15/19)

CTGGTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGCCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAG  >  NC_000913/1665639‑1665904                                                                                                                                   |                                                                                                                                        ctgGTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCggg                                                                                                                                <  1:318770/140‑1 (MQ=255)  tgGTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGa                                                                                                                               <  1:687126/140‑1 (MQ=255)   ggTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGaa                                                                                                                              >  1:98264/1‑140 (MQ=255)       gtaACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGGAtatc                                                                                                                          >  2:166090/1‑137 (MQ=255)         aaCCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTg                                                                                                                        >  1:180448/1‑140 (MQ=255)         aaCCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTg                                                                                                                        >  1:24415/1‑140 (MQ=255)                ggCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTa                                                                                                                 <  1:721883/140‑1 (MQ=255)                 gCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGaa                                                                                                                              >  2:812268/1‑126 (MQ=255)                 gCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGaa                                                                                                                              <  1:812268/126‑1 (MQ=255)                  cAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCg                                                                                                                      >  1:220052/1‑133 (MQ=255)                  cAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCg                                                                                                                      <  2:220052/133‑1 (MQ=255)                  cAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATc                                                                                                               >  2:873996/1‑140 (MQ=255)                  cAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGT‑GAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGGGGCCCATCg                                                                                                              >  1:246163/1‑140 (MQ=255)                    gTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCgg                                                                                                             >  2:389272/1‑139 (MQ=255)                      gCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGGCTaacccgc                                                                                                           >  2:974281/1‑134 (MQ=255)                       cAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGcc                                                                                                          >  1:495096/1‑140 (MQ=255)                               cgcgAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGc                                                                                                  >  1:553660/1‑140 (MQ=255)                                gcgAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCt                                                                                                 <  1:325981/140‑1 (MQ=255)                                   aaGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGc                                                                                              <  2:818802/140‑1 (MQ=255)                                              atTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTttatttat                                                                                   <  2:253721/140‑1 (MQ=255)                                                 cTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCTCCGCTTGCTGGCAGTTTATTTATAGc                                                                                <  2:624547/140‑1 (MQ=255)                                                                gccgccTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTgg                                                                 <  1:873996/140‑1 (MQ=255)                                                                gccgccTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTgg                                                                 <  2:180448/140‑1 (MQ=255)                                                                     ctcctGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTa                                                            <  1:166090/140‑1 (MQ=255)                                                                     ctcctGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTa                                                            <  1:974281/140‑1 (MQ=255)                                                                                cccAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGGTGCGGCCTATCGGGCCCCGCTTGCTGGCCGTTTATTTATAGCCGAAGTGCTGTTTGGCACTTTGATGTTGGcc                                                 >  2:311166/1‑140 (MQ=255)                                                                                   aaCGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGTGGCGGGAAAGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTTTTTATAGCCGAAGTGCTGTTTGGGACTATTATGTTGGGctct                                              >  2:905073/1‑140 (MQ=255)                                                                                     cGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGc                                                  <  2:927929/134‑1 (MQ=255)                                                                                     cGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGc                                                  >  1:927929/1‑134 (MQ=255)                                                                                                         gTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGGAGGGCTGCGGCCTATCGGGGCCCGCTTGCTGGCAGCTTATTTATAACCCGAGTGGCGTTTGGGACTATGATGTTGGCCTCTCTCGGCCCGGGGGTTAttttcc                        >  2:878131/1‑137 (MQ=255)                                                                                                         gTGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTccg                        <  2:24415/140‑1 (MQ=255)                                                                                                          tGGAAATTATGGATCGCCTGTGGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTccgc                       <  2:495096/140‑1 (MQ=255)                                                                                                                         gCCTGTGGGGGCGTGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCt        <  1:905073/140‑1 (MQ=255)                                                                                                                             gtgGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCcgtcgt                 <  1:836328/127‑1 (MQ=255)                                                                                                                             gtgGGGGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCcgtcgt                 >  2:836328/1‑127 (MQ=255)                                                                                                                               ggggGCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAg  <  1:985933/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        CTGGTAGTAACCAGCGGCAGTGCAATTGGTCGCGAAGGTGCGATGATTCTTTTAGCTGCCCTTGCCGCCTCCTGTTTTGCCCAACGTTTTACGCCACGCCAGGAGTGGAAATTATGGATCGCCTGTGGGGCCGCGGCGGGAATGGCTGCGGCCTATCGTGCCCCGCTTGCTGGCAGTTTATTTATAGCCGAAGTGCTGTTTGGCACTATGATGTTGGCCTCTCTCGGCCCGGTGATTATTTCCGCCGTCGTGGCATTGCTGGTTAG  >  NC_000913/1665639‑1665904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑