Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,734	T→C	V103A (GTA→GCA)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,734	0	T	C	100.0%	164.6 / NA	47	V103A (GTA→GCA)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

GTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGAAAGG  >  NC_000913/1678599‑1678869                                                                                                                                        |                                                                                                                                        gtcgCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAAtt                                                                                                                                     <  1:302902/140‑1 (MQ=255)   cgcgCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTaa                                                                                                                                   <  1:878997/140‑1 (MQ=255)      gcAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACgg                                                                                                                                <  1:469726/140‑1 (MQ=255)      gcAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCCGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACgg                                                                                                                                <  2:559541/140‑1 (MQ=255)                  ggTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACt                                                                                                                    >  2:480263/1‑140 (MQ=255)                    tGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGc                                                                                                                  >  2:560970/1‑140 (MQ=255)                    tGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGc                                                                                                                  >  2:618025/1‑140 (MQ=255)                    tGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGc                                                                                                                  >  1:391625/1‑140 (MQ=255)                    tGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGc                                                                                                                  >  1:770551/1‑140 (MQ=255)                    tGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGc                                                                                                                  >  1:556868/1‑140 (MQ=255)                      ccgccTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCg                                                                                                                >  1:561005/1‑140 (MQ=255)                         ccTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGaaa                                                                                                             >  2:37899/1‑140 (MQ=255)                           tctTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGa                                                                                                           <  2:830662/140‑1 (MQ=255)                                ttATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAgg                                                                                                      <  1:480263/140‑1 (MQ=255)                                       gtCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCt                                                                                               >  1:703513/1‑140 (MQ=255)                                              cTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGaa                                                                                        <  1:37899/140‑1 (MQ=255)                                                 cTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTATTCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCg                                                                                     >  2:862796/1‑140 (MQ=255)                                                       ttGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGac                                                                               >  1:836547/1‑140 (MQ=255)                                                       ttGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGac                                                                               >  1:470224/1‑140 (MQ=255)                                                         ggTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGacac                                                                             >  2:125646/1‑140 (MQ=255)                                                           tAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGacac                                                                             >  1:722110/1‑138 (MQ=255)                                                           tAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGacac                                                                             <  2:722110/138‑1 (MQ=255)                                                              cAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCa                                                                        <  1:125646/140‑1 (MQ=255)                                                              cAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCa                                                                        <  2:556868/140‑1 (MQ=255)                                                              cAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCa                                                                        >  2:612099/1‑140 (MQ=255)                                                              cAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCa                                                                        <  1:491039/140‑1 (MQ=255)                                                              cAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCa                                                                        <  1:618025/140‑1 (MQ=255)                                                                       cTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAgg                                                               <  2:864450/140‑1 (MQ=255)                                                                           cGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCtt                                                           <  1:614286/140‑1 (MQ=255)                                                                                    gCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTag                                                  <  2:836547/140‑1 (MQ=255)                                                                                    gCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTag                                                  <  2:470224/140‑1 (MQ=255)                                                                                     cTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTagc                                                 <  2:561005/140‑1 (MQ=255)                                                                                         ccTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTagccagc                                             <  2:355354/140‑1 (MQ=255)                                                                                               taAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAg                                       >  1:490976/1‑140 (MQ=255)                                                                                                aaaGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGt                                      <  2:700291/140‑1 (MQ=255)                                                                                                    ccGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAat                                  >  2:498298/1‑140 (MQ=255)                                                                                                       aTGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAatgat                               >  1:128056/1‑140 (MQ=255)                                                                                                        tGCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAatgatg                              <  2:490976/140‑1 (MQ=255)                                                                                                         gCTGAAAAAGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGc                             <  1:645739/140‑1 (MQ=255)                                                                                                              aaaaGCAGAAGAAACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCa                        >  2:437257/1‑140 (MQ=255)                                                                                                                       agaaACAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCgg               >  2:877377/1‑140 (MQ=255)                                                                                                                         aaaCAAGTAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCa             <  1:352716/140‑1 (MQ=255)                                                                                                                                tAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGa      >  2:440530/1‑140 (MQ=255)                                                                                                                                tAATCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGa      >  2:140233/1‑140 (MQ=255)                                                                                                                                   tCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGAAAg   >  1:309976/1‑140 (MQ=255)                                                                                                                                   tCGCGCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGAAAg   >  1:133769/1‑140 (MQ=255)                                                                                                                                    cgcgCAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGAAAgg  >  2:55609/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        GTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACGGTGTTGTCGAACTGCCGAAAGATCAGGCTGTTCTGATTGAACCGTTTGACACGGTCACCGTCCAGGGCTTCTATCGTAGCCAGCCAGAAGTCAATGATGCCATCACCAAAGCGGCAAAAGCGAAAGG  >  NC_000913/1678599‑1678869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑