Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,337	T→C	G105G (GGT→GGC)	nemR →	DNA‑binding transcriptional repressor NemR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,337	0	T	C	100.0%	215.4 / NA	61	G105G (GGT→GGC)	nemR	DNA‑binding transcriptional repressor NemR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/34);  total (27/34)

GAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTT  >  NC_000913/1726203‑1726470                                                                                                                                       |                                                                                                                                      gAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCt                                                                                                                                  <  1:825897/140‑1 (MQ=255)   gCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGa                                                                                                                                <  1:511013/140‑1 (MQ=255)        aTTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTa                                                                                                                           >  2:230694/1‑140 (MQ=255)             gccgcATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAAct                                                                                                                      <  2:876198/140‑1 (MQ=255)               cgcATATCGCCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAGCCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAActct                                                                                                                    <  1:48926/140‑1 (MQ=255)                 cATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCt                                                                                                                                  <  2:827578/124‑1 (MQ=255)                 cATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCt                                                                                                                                  >  1:827578/1‑124 (MQ=255)                 cATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAActctct                                                                                                                  >  1:101129/1‑140 (MQ=255)                 cATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAActctct                                                                                                                  >  2:541137/1‑140 (MQ=255)                   tatCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGc                                                                                                                >  2:434414/1‑140 (MQ=255)                     tCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCg                                                                                                              <  2:131719/140‑1 (MQ=255)                      cACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAAct                                                                                                                      >  2:260740/1‑131 (MQ=255)                      cACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAAct                                                                                                                      <  1:260740/131‑1 (MQ=255)                       aCCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGaa                                                                                                            <  2:883755/140‑1 (MQ=255)                              actgactgAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCg                                                                                                              >  2:145731/1‑131 (MQ=255)                              actgactgAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCg                                                                                                              <  1:145731/131‑1 (MQ=255)                              actgactgAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGa                                                                                                             >  1:67141/1‑132 (MQ=255)                              actgactgAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGa                                                                                                             <  2:67141/132‑1 (MQ=255)                              actgactgAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACT‑GCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGa                                                                                                    >  1:361484/1‑140 (MQ=255)                               ctgactgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACT‑GCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGAt                                                                                                   <  2:361484/140‑1 (MQ=255)                                tgactgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGAt                                                                                                   <  1:230694/140‑1 (MQ=255)                                tgactgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGAt                                                                                                   <  2:617731/140‑1 (MQ=255)                                tgactgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGAt                                                                                                   <  2:718698/140‑1 (MQ=255)                                  actgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCt                                                                                                 <  2:971862/140‑1 (MQ=255)                                   ctgaGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTg                                                                                                >  1:696221/1‑140 (MQ=255)                                            tgcAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGat                                                                                       >  2:675145/1‑140 (MQ=255)                                            tgcAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGat                                                                                       >  1:590295/1‑140 (MQ=255)                                            tgcAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGat                                                                                       >  1:576692/1‑140 (MQ=255)                                                 tCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATgcg                                                                                  <  2:791692/140‑1 (MQ=255)                                                   cGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCa                                                                                <  2:143660/140‑1 (MQ=255)                                                   cGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCa                                                                                <  2:696221/140‑1 (MQ=255)                                                     gCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAgc                                                                              >  1:350770/1‑140 (MQ=255)                                                      cGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAgcg                                                                             >  1:714747/1‑140 (MQ=255)                                                        aaGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGcgcg                                                                           <  2:101129/140‑1 (MQ=255)                                                                  ccGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGcgcg                                                                           <  1:428249/130‑1 (MQ=255)                                                                  ccGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGcgcg                                                                           >  2:428249/1‑130 (MQ=255)                                                                       aCCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCt                                                            <  1:150937/140‑1 (MQ=255)                                                                          gCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGACTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTcgc                                                         >  2:977601/1‑140 (MQ=255)                                                                            aTACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCgcgg                                                       <  2:662075/140‑1 (MQ=255)                                                                              aCTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCgcggcg                                                     <  1:541137/140‑1 (MQ=255)                                                                                 ggCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGa                                                  <  2:209399/140‑1 (MQ=255)                                                                                  gCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAAGCATCAGTGGCTGCCTGACGGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGAt                                                 >  2:877283/1‑140 (MQ=255)                                                                                    ttattaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAGGGTGCTCGCGGCGTGATCg                                               <  1:421644/140‑1 (MQ=255)                                                                                      attaCCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc                                             >  2:933030/1‑140 (MQ=255)                                                                                            aGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGctc                                       >  2:19068/1‑140 (MQ=255)                                                                                                aaCACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCtctctc                                   <  1:434414/140‑1 (MQ=255)                                                                                                 acacTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc                                             <  2:28852/129‑1 (MQ=255)                                                                                                 acacTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGcc                                             >  1:28852/1‑129 (MQ=255)                                                                                                  cacTGAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAg                                 >  2:545211/1‑140 (MQ=255)                                                                                                      gAACCAGTTTTGCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGgcgc                             <  1:545211/140‑1 (MQ=255)                                                                                                                 gCCAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGc                  >  2:699528/1‑140 (MQ=255)                                                                                                                  ccAACATGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGcc                 <  2:197855/140‑1 (MQ=255)                                                                                                                       aTGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGTGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTgag            <  2:576692/140‑1 (MQ=255)                                                                                                                       aTGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTgag            <  2:350770/140‑1 (MQ=255)                                                                                                                       aTGGAACCATCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTgag            <  1:19068/140‑1 (MQ=255)                                                                                                                                tCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGTGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGt   >  2:311345/1‑140 (MQ=255)                                                                                                                                tCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGt   >  2:776277/1‑140 (MQ=255)                                                                                                                                tCAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGt   >  2:488554/1‑140 (MQ=255)                                                                                                                                 cAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGcc                 >  1:436522/1‑125 (MQ=255)                                                                                                                                 cAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGcc                 <  2:436522/125‑1 (MQ=255)                                                                                                                                 cAGTGGCTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGtt  <  1:488554/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTGGTTGCCTGACAGTAAAACTCTCTGCCGAAGTGTGCGATCTGTCAGAAGATATGCGCAGCGCGATGGATAAAGGTGCTCGCGGCGTGATCGCCCTGCTCTCTCAGGCGCTGGAAAATGGCCGTGAGAACCATTGTT  >  NC_000913/1726203‑1726470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑