Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,760,542	C→T	R417Q (CGG→CAG)	sufD ←	Fe‑S cluster scaffold complex subunit SufD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,760,542	0	C	T	100.0%	110.9 / NA	32	R417Q (CGG→CAG)	sufD	Fe‑S cluster scaffold complex subunit SufD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/19);  total (13/19)

CCTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGA  >  NC_000913/1760409‑1760674                                                                                                                                      |                                                                                                                                     ccTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGAc                                                                                                                                >  2:440395/1‑140 (MQ=255)      cTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGAtt                                                                                                                           <  2:419034/140‑1 (MQ=255)        cGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCg                                                                                                                         <  1:930519/140‑1 (MQ=255)         ggTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCgg                                                                                                                        >  1:440285/1‑140 (MQ=255)              cTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAg                                                                                                                   <  2:883242/140‑1 (MQ=255)                           gcgcTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGATAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTa                                                                                                      <  2:679725/140‑1 (MQ=255)                              cTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGc                                                                                                   >  1:383481/1‑140 (MQ=255)                               tGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGcc                                                                                                  <  1:533609/140‑1 (MQ=255)                                gTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGccc                                                                                                 >  1:900505/1‑140 (MQ=255)                                         aGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGcccc                                                                                                <  1:884158/132‑1 (MQ=255)                                         aGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGcccc                                                                                                >  2:884158/1‑132 (MQ=255)                                         aGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACg                                                                                        <  1:7307/140‑1 (MQ=255)                                         aGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACg                                                                                        <  1:91763/140‑1 (MQ=255)                                         aGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGACCAGCACCTGCTGTTTAAGCCCCTCATCACg                                                                                        <  1:920016/140‑1 (MQ=255)                                           ccAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCa                                                                                      <  1:208444/140‑1 (MQ=255)                                                ggCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCt                                                                                 >  2:755751/1‑140 (MQ=255)                                                                     gAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAg                                                            <  2:383481/140‑1 (MQ=255)                                                                             cGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTaaa                                                     >  1:980973/1‑139 (MQ=255)                                                                             cGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTaaa                                                     <  2:980973/139‑1 (MQ=255)                                                                                    tCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGatcatc                                             >  2:848599/1‑140 (MQ=255)                                                                                       gCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATctgc                                          >  2:984667/1‑140 (MQ=255)                                                                                                 tGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCct                                >  1:525327/1‑140 (MQ=255)                                                                                                  gTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCctg                               >  2:491441/1‑140 (MQ=255)                                                                                                  gTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCctg                               <  2:900505/140‑1 (MQ=255)                                                                                                        ggAAAAAATCATCTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCtgatt                         <  2:525327/140‑1 (MQ=255)                                                                                                                 catcTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCcgcg                <  1:440395/140‑1 (MQ=255)                                                                                                                    cTTGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGa             >  1:615614/1‑140 (MQ=255)                                                                                                                     ttGCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAg            <  1:755751/140‑1 (MQ=255)                                                                                                                       gCACCTCCTGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAgcg          <  2:405920/140‑1 (MQ=255)                                                                                                                         ccctcctGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCa        <  2:159065/139‑1 (MQ=255)                                                                                                                           ctcctGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGa      <  1:491441/140‑1 (MQ=255)                                                                                                                               tGGCAGCTGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGa  >  2:520730/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     CCTGGCTCGGTTTCTGCGCACTGGCGGCGCTGTCGAGATAAGCCAGCGGCAAACCGTTTACCTCACGCGAAAGCACCGGAAAGTCGGCCCGCACTTTGTCGACGGAAAAAATCATCTTGCACCTCCTGGCAGCCGTTGACCGATTCGGGCCAGCACCTGCTGTTTAAGCCCCTCATCACGCAGTGCTTCCGTCAGTTCGGCAGCGAAGGCGTAAATGATCATCTGCTGGGCATCCTGCTGATTGATCCCGCGCGAGCGCAGATAGA  >  NC_000913/1760409‑1760674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑