Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,814	C→T	A383T (GCC→ACC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,814	0	C	T	100.0%	235.8 / NA	69	A383T (GCC→ACC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/33);  total (36/33)

TTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTT  >  NC_000913/1845683‑1845948                                                                                                                                    |                                                                                                                                       ttCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTgcg                                                                                                                                >  2:867809/1‑140 (MQ=255)           cacTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCg                                                                                                                           >  1:80619/1‑135 (MQ=255)           cacTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCg                                                                                                                           <  2:80619/135‑1 (MQ=255)              ttGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCttttt                                                                                                                   >  2:179218/1‑140 (MQ=255)              ttGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCttttt                                                                                                                   <  2:120977/140‑1 (MQ=255)                gCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGt                                                                                                                 >  1:144001/1‑140 (MQ=255)                      cacCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCAt                                                                                                              <  1:528779/137‑1 (MQ=255)                      cacCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCAt                                                                                                              >  2:528779/1‑137 (MQ=255)                        ccGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCaa                                                                                                         >  2:519555/1‑140 (MQ=255)                           cATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAg                                                                                                      >  1:223327/1‑140 (MQ=255)                           cATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAg                                                                                                      >  1:795330/1‑140 (MQ=255)                           cATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAg                                                                                                      >  1:779076/1‑140 (MQ=255)                              ccGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCgg                                                                                                   >  1:217246/1‑140 (MQ=255)                               cGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGt                                                                                                  <  2:330209/140‑1 (MQ=255)                                 ggCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATccc                                                                                                           >  2:130651/1‑129 (MQ=255)                                 ggCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATccc                                                                                                           <  1:130651/129‑1 (MQ=255)                                 ggCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTg                                                                                                >  1:142833/1‑140 (MQ=255)                                  gCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGc                                                                                               >  1:212381/1‑140 (MQ=255)                                  gCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGc                                                                                               <  2:223327/140‑1 (MQ=255)                                   cAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAg                                                                                                      <  1:640040/132‑1 (MQ=255)                                   cAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAg                                                                                                      >  2:640040/1‑132 (MQ=255)                                   cAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCg                                                                                              <  1:519555/140‑1 (MQ=255)                                   cAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCg                                                                                              <  2:706152/140‑1 (MQ=255)                                   cAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCg                                                                                              <  2:144001/140‑1 (MQ=255)                                    aGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGt                                                                                             <  1:179218/140‑1 (MQ=255)                                    aGAATTACATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACC‑TTTTGTCATCCCAACAGCGGTTGCGta                                                                                            <  2:857947/140‑1 (MQ=255)                                              cAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGAt                                                                                   <  2:616492/140‑1 (MQ=255)                                              cAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGAt                                                                                   <  1:645744/140‑1 (MQ=255)                                                gATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGAt                                                                                   <  2:455463/138‑1 (MQ=255)                                                gATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGAt                                                                                   >  1:455463/1‑138 (MQ=255)                                                     tGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATc                                                                                        <  2:352770/128‑1 (MQ=255)                                                     tGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATc                                                                                        >  1:352770/1‑128 (MQ=255)                                                      gACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACC‑TTTTGTCATCCCAACAGCGGTTGCGTATATCt                                                                                       >  2:887411/1‑127 (MQ=255)                                                      gACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACC‑TTTTGTCATCCCAACAGCGGTTGCGTATATCt                                                                                       <  1:887411/127‑1 (MQ=255)                                                          ggCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCt                                                                       >  1:455680/1‑140 (MQ=255)                                                           gCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTg                                                                      >  2:144745/1‑140 (MQ=255)                                                            cAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGc                                                                     >  2:867046/1‑140 (MQ=255)                                                            cAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGc                                                                     >  2:608413/1‑140 (MQ=255)                                                             aaTCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCg                                                                    <  2:455680/140‑1 (MQ=255)                                                               tCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGc                                                                  <  2:712558/140‑1 (MQ=255)                                                               tCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGc                                                                  <  2:795330/140‑1 (MQ=255)                                                                       taGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTAGCACTGGCTGCGGCAACAGATc                                                          <  2:698460/140‑1 (MQ=255)                                                                       taGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATc                                                          >  2:615111/1‑140 (MQ=255)                                                                          aCCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCa                                                                 >  1:716709/1‑130 (MQ=255)                                                                          aCCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCa                                                                 <  2:716709/130‑1 (MQ=255)                                                                            cttcgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTg                                                     <  1:909952/140‑1 (MQ=255)                                                                              tcgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCa                                                   >  2:843749/1‑140 (MQ=255)                                                                              tcgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCa                                                   <  2:476045/140‑1 (MQ=255)                                                                              tcgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCa                                                   >  1:476045/1‑140 (MQ=255)                                                                               cgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCAt                                                  >  1:413215/1‑140 (MQ=255)                                                                               cgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCAt                                                  >  2:718930/1‑140 (MQ=255)                                                                               cgcttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCAt                                                  >  2:345136/1‑140 (MQ=255)                                                                                 cttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGa                                                <  2:217246/140‑1 (MQ=255)                                                                                 cttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGa                                                >  2:246285/1‑140 (MQ=255)                                                                                  ttcgTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGaa                                               <  2:142833/140‑1 (MQ=255)                                                                                          ccGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATg                                       <  2:215920/140‑1 (MQ=255)                                                                                               aGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCAtt                                  <  1:608413/140‑1 (MQ=255)                                                                                                ggTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATg                                       >  2:468505/1‑134 (MQ=255)                                                                                                ggTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATg                                       <  1:468505/134‑1 (MQ=255)                                                                                                 gTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATgca                                >  2:604608/1‑140 (MQ=255)                                                                                                                ccGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTc                 >  1:205553/1‑140 (MQ=255)                                                                                                                ccGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTc                 >  2:418145/1‑140 (MQ=255)                                                                                                                 cGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTcc                >  2:232416/1‑140 (MQ=255)                                                                                                                 cGTGCGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTcc                >  2:197685/1‑140 (MQ=255)                                                                                                                     cGGTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCa            <  1:852259/140‑1 (MQ=255)                                                                                                                      ggTCGGAATGATGGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCaa           >  2:422033/1‑140 (MQ=255)                                                                                                                             atgatgGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGttc    <  1:604608/140‑1 (MQ=255)                                                                                                                               gatgGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGttctt  <  2:205553/140‑1 (MQ=255)                                                                                                                               gatgGTGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGttctt  <  1:368777/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATCAGGTTATAGACCTTCGCTTCGTTCTCCGTCAGGTTGATCGCAGAACTCCGTGCGGTCGGAATGATGGCGTGGTGCGCATCGACCTTTTTGTCATCCCAACAGCGGTTGCGTATATCTGGATCTACCACTGGCTGCGGCAACAGATCCGGTGCATGAACACTGATGGCATTCATCACCGCGTGGCGTCCGGCAAAATGTTCTT  >  NC_000913/1845683‑1845948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑