Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,944,415	T→C	T314T (ACA→ACG)	ruvB ←	Holliday junction branch migration complex subunit RuvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,944,415	0	T	C	100.0%	175.9 / NA	50	T314T (ACA→ACG)	ruvB	Holliday junction branch migration complex subunit RuvB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

TAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAG  >  NC_000913/1944278‑1944549                                                                                                                                          |                                                                                                                                       tAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGgcgt                                                                                                                                      >  2:535869/1‑140 (MQ=255)     aCGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTAc                                                                                                                                    <  2:63785/138‑1 (MQ=255)     aCGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTAc                                                                                                                                    >  1:63785/1‑138 (MQ=255)     aCGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGc                                                                                                                                  >  1:150634/1‑140 (MQ=255)     aCGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGc                                                                                                                                  >  2:892551/1‑140 (MQ=255)     aCGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGc                                                                                                                                  <  1:723725/140‑1 (MQ=255)          aaCAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCaa                                                                                                                             >  1:473026/1‑140 (MQ=255)           aCAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCaaa                                                                                                                            <  2:174174/140‑1 (MQ=255)               cGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGc                                                                                                                        >  1:577917/1‑140 (MQ=255)               cGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGc                                                                                                                        >  1:627441/1‑140 (MQ=255)                        aGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGaa                                                                                                               <  1:585204/140‑1 (MQ=255)                              atTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAAt                                                                                                         <  2:473026/140‑1 (MQ=255)                               ttGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGaa                                                                                                               <  2:624915/133‑1 (MQ=255)                               ttGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGaa                                                                                                               >  1:624915/1‑133 (MQ=255)                                tGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATaa                                                                                                       <  1:281856/140‑1 (MQ=255)                                 gCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAg                                                                                                      <  1:535869/140‑1 (MQ=255)                                    aGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATa                                                                                                        >  2:963754/1‑135 (MQ=255)                                    aGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATa                                                                                                        <  1:963754/135‑1 (MQ=255)                                        ccGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAg                                                                                               >  1:482457/1‑140 (MQ=255)                                         cGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGc                                                                                              >  1:674472/1‑140 (MQ=255)                                         cGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGc                                                                                              <  2:115829/140‑1 (MQ=255)                                          ggATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGca                                                                                             <  1:788886/140‑1 (MQ=255)                                           gATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGcac                                                                                            <  1:601004/140‑1 (MQ=255)                                            aTGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGcaca                                                                                           <  1:892551/140‑1 (MQ=255)                                            aTGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGcaca                                                                                           >  1:887560/1‑140 (MQ=255)                                            aTGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGca                                                                                             >  2:817651/1‑138 (MQ=255)                                            aTGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGca                                                                                             <  1:817651/138‑1 (MQ=255)                                                           ccAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCa                                                                            <  2:88093/140‑1 (MQ=255)                                                           ccAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCa                                                                            <  2:482457/140‑1 (MQ=255)                                                                 cGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGttct                                                                      <  1:667451/140‑1 (MQ=255)                                                                            aTTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGc                                                           <  2:150634/140‑1 (MQ=255)                                                                             tttCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCt                                                          <  2:276141/140‑1 (MQ=255)                                                                               tCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTgc                                                        >  1:183341/1‑140 (MQ=255)                                                                               tCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTgc                                                        >  2:972155/1‑140 (MQ=255)                                                                                           aTGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATc                                            >  2:524952/1‑140 (MQ=255)                                                                                            tGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATcc                                           <  2:413253/140‑1 (MQ=255)                                                                                                 aaGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTCCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCa                                          <  2:951026/136‑1 (MQ=255)                                                                                                 aaGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTCCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCa                                          >  1:951026/1‑136 (MQ=255)                                                                                                  aGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCt                                     >  2:23233/1‑140 (MQ=255)                                                                                                   gTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTa                                    >  1:14085/1‑140 (MQ=255)                                                                                                   gTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTa                                    >  2:828259/1‑140 (MQ=255)                                                                                                   gTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTa                                    <  1:930742/140‑1 (MQ=255)                                                                                                      aTTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAg                                 >  2:833235/1‑140 (MQ=255)                                                                                                      aTTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAg                                 >  1:26391/1‑140 (MQ=255)                                                                                                         ccACGCCCGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTc                              <  1:833235/140‑1 (MQ=255)                                                                                                              cccGCGTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTccacca                         >  1:801149/1‑140 (MQ=255)                                                                                                                cgcgTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCaaa                       <  2:14085/140‑1 (MQ=255)                                                                                                                 gcgTCGTCGCCATACGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAg                      <  2:26391/140‑1 (MQ=255)                                                                                                                               cGCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTAc        >  1:67631/1‑140 (MQ=255)                                                                                                                                gCCCACGCGGCGTACGCTGCAAAAAGCCTTGCTGAACCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTAcc       <  1:460925/140‑1 (MQ=255)                                                                                                                                     cgcgGCGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAg  >  1:615387/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TAAGACGCGAACAGCGTCGCATCAGGCATATTGCCAGTGCCGGATGCGGCGCGAGCGACCAATCCGACTTACGGCATTTCTGGCGGCGTTATGCCAAAGTGATTCCACGCCCGCGTCGTCGCCATACGCCCACGCGGTGTACGCTGCAAAAAGCCTTGCTGAATCAAATAAGGTTCCAGCACATCCTCAATGGTTTCACGTTCTTCGCCAATGGCTGCCGCCAGGTTATCCAGACCTACAGGTCCACCAAAGAACTTATCGATTACCGCCAG  >  NC_000913/1944278‑1944549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑