Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,983,101	T→C	Y147C (TAC→TGC)	araH ←	arabinose ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,983,101	0	T	C	100.0%	142.0 / NA	41	Y147C (TAC→TGC)	araH	arabinose ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

TTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGCCACGCCAATCCA  >  NC_000913/1982966‑1983234                                                                                                                                        |                                                                                                                                      ttCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGc                                                                                                                                   >  2:479538/1‑140 (MQ=255)    agcagcAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAg                                                                                                                                <  2:532801/140‑1 (MQ=255)      cagcaAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGcc                                                                                                                                  <  2:168881/136‑1 (MQ=255)      cagcaAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGcc                                                                                                                                  >  1:168881/1‑136 (MQ=255)      cagcaAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGAc                                                                                                                              >  2:677449/1‑140 (MQ=255)         caAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTc                                                                                                                           >  2:565803/1‑140 (MQ=255)           aaCCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGa                                                                                                                         <  1:493292/140‑1 (MQ=255)            aCCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGaa                                                                                                                        <  2:25478/140‑1 (MQ=255)                     aaTCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCa                                                                                                               <  1:565803/140‑1 (MQ=255)                             acGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGcc                                                                                                       >  1:448041/1‑140 (MQ=255)                              cgcgACGGTGAGGCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCa                                                                                                      <  1:792158/140‑1 (MQ=255)                              cgcgACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCa                                                                                                      <  1:780038/140‑1 (MQ=255)                              cgcgACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCa                                                                                                      <  2:628243/140‑1 (MQ=255)                              cgcgACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCa                                                                                                      <  1:677449/140‑1 (MQ=255)                                cgACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAAt                                                                                                    >  1:927903/1‑140 (MQ=255)                                   cGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTc                                                                                                 <  2:448041/140‑1 (MQ=255)                                      tGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTg                                                                                              <  1:247419/140‑1 (MQ=255)                                                tGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCat                                                                                    <  2:927903/140‑1 (MQ=255)                                                  gcgcAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCattt                                                                                  >  1:978714/1‑140 (MQ=255)                                                  gcgcAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCattt                                                                                  >  1:193901/1‑140 (MQ=255)                                                        gcagACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTc                                                                            >  2:835762/1‑140 (MQ=255)                                                            aCCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGtt                                                                        >  2:661070/1‑140 (MQ=255)                                                            aCCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGtt                                                                        >  2:383630/1‑140 (MQ=255)                                                                aaCCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGc                                                                    >  2:117336/1‑140 (MQ=255)                                                                      ttGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGAt                                                                 <  1:431790/137‑1 (MQ=255)                                                                      ttGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGAt                                                                 >  2:431790/1‑137 (MQ=255)                                                                      ttGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAAc                                                              >  1:424179/1‑140 (MQ=255)                                                                      ttGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAAc                                                              >  2:729390/1‑140 (MQ=255)                                                                             aaCCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCa                                                       <  1:766652/140‑1 (MQ=255)                                                                                  aGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGAc                                                  <  2:369610/140‑1 (MQ=255)                                                                                   gggCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGAcc                                                 <  2:424179/140‑1 (MQ=255)                                                                                              cTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACagag                                      >  1:275462/1‑140 (MQ=255)                                                                                              cTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACagag                                      >  1:346465/1‑140 (MQ=255)                                                                                               tttCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAgaga                                     <  2:346465/140‑1 (MQ=255)                                                                                                  cATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCACGCCACAGAGAACg                                  <  1:479538/140‑1 (MQ=255)                                                                                                                accgCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAAccc                    >  1:548370/1‑140 (MQ=255)                                                                                                                accgCTTTACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAAccc                    >  1:510845/1‑140 (MQ=255)                                                                                                                      ttACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGc              <  2:585459/140‑1 (MQ=255)                                                                                                                       tACCGTCTGAAATGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGcc             >  1:46799/1‑140 (MQ=255)                                                                                                                                aaaTGATGCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGCCACGCCAATc    <  1:383630/140‑1 (MQ=255)                                                                                                                                  atgatgCACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGCCACGCCAATCca  <  2:193901/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAGATTGGCGCAGGCAGACCGAACCAGTTGGCGTAACCAAGGGCAAAGAAGCTTTCATCTTCGATACCGACCGCTTTACCGTCTGAAATGATGTACGCCAGACCTCGAACAATCTGCATCGTTGCCAATGTCGTGATCAGAGCATTTATTTTCAGTTTGGCGATAACAAAGCCATTGACCAGGCCACAGAGAACGCCCAGCAACAACCCCGCTGCCACGCCAATCCA  >  NC_000913/1982966‑1983234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑