Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,997,280	G→A	L179L (CTG→TTG)	tcyN ←	cystine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,997,280	0	G	A	100.0%	132.6 / NA	40	L179L (CTG→TTG)	tcyN	cystine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/15);  total (25/15)

CTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAGCTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAG  >  NC_000913/1997148‑1997415                                                                                                                                     |                                                                                                                                        cTATCCGCCCCTGGTACATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGa                                                                                                                                  >  2:664036/1‑140 (MQ=255)   aTCCGCCCCTGGTCCATAAAGAGCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATc                                                                                                                                <  1:695080/140‑1 (MQ=255)    tCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATcc                                                                                                                               >  2:131063/1‑140 (MQ=255)           cTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTg                                                                                                                        >  1:606082/1‑140 (MQ=255)              gTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACg                                                                                                                     >  1:399867/1‑140 (MQ=255)              gTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGCGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACg                                                                                                                     >  1:433022/1‑140 (MQ=255)                   tAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAg                                                                                                                             <  1:763888/127‑1 (MQ=255)                   tAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAg                                                                                                                             >  2:763888/1‑127 (MQ=255)                        aTCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAgcg                                                                                                                           <  1:300557/124‑1 (MQ=255)                        aTCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAgcg                                                                                                                           >  2:300557/1‑124 (MQ=255)                        aTCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTc                                                                                                           >  2:214157/1‑140 (MQ=255)                            cccGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTcaaac                                                                                                       >  1:644307/1‑140 (MQ=255)                                 tCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAAt                                                                                                  <  1:131063/140‑1 (MQ=255)                                          tCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAgg                                                                                         >  2:739976/1‑140 (MQ=255)                                           cccGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAg                                                                                          >  1:621949/1‑138 (MQ=255)                                           cccGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAg                                                                                          <  2:621949/138‑1 (MQ=255)                                           cccGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGa                                                                                        >  1:512868/1‑140 (MQ=255)                                             cGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACg                                                                                      >  1:445540/1‑140 (MQ=255)                                                  aaaGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTg                                                                                 <  1:214157/140‑1 (MQ=255)                                                  aaaGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTg                                                                                 >  1:372266/1‑140 (MQ=255)                                                               tGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACgcgc                                                                    <  1:739976/140‑1 (MQ=255)                                                                       aaTCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCaa                                                            <  2:644307/140‑1 (MQ=255)                                                                               tCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACgctgc                                                    <  1:908586/140‑1 (MQ=255)                                                                                cGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACgctgct                                                   >  2:977996/1‑140 (MQ=255)                                                                                  tGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCaa                                                            <  1:780092/129‑1 (MQ=255)                                                                                  tGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCaa                                                            >  2:780092/1‑129 (MQ=255)                                                                                   gcgcTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGtt                                                <  2:512868/140‑1 (MQ=255)                                                                                             tGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGa                                      <  1:356944/140‑1 (MQ=255)                                                                                               cgcCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACa                                    >  2:152554/1‑140 (MQ=255)                                                                                               cgcCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACa                                    >  2:170548/1‑140 (MQ=255)                                                                                                   aGCTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAacg                                <  1:73215/140‑1 (MQ=255)                                                                                                     cTGACGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAacgac                              >  2:735605/1‑140 (MQ=255)                                                                                                         cGGATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTgg                          >  2:482270/1‑140 (MQ=255)                                                                                                          ggATGGTGTTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGa                         <  2:399867/140‑1 (MQ=255)                                                                                                               gtgtTCAGGACTTCACCCACCAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCt                    >  2:59670/1‑140 (MQ=255)                                                                                                                            cacccaccAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCt       >  1:55658/1‑140 (MQ=255)                                                                                                                            cacccaccAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCt       >  1:271049/1‑140 (MQ=255)                                                                                                                            cacccaccAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCt       >  1:923737/1‑140 (MQ=255)                                                                                                                            cacccaccAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCt       <  1:735605/140‑1 (MQ=255)                                                                                                                              cccaccAACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGc     <  1:664036/140‑1 (MQ=255)                                                                                                                                 accaACTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAg  >  1:336154/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGACTTCACCCACCAGCTCTGGATCCAGCGCTGACGTTGGCTCGTCAAACAAAATCACCTCAGGACGCATTGCCAGCGCACGCGCAATCGCAACACGCTGCTGTTGACCGCCAGACAAACGACGTGGATAGCTGGTTTCTTTACCTGCCAG  >  NC_000913/1997148‑1997415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑