Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,056,328	T→C	L423P (CTA→CCA)	amn →	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,056,328	0	T	C	100.0%	130.7 / NA	38	L423P (CTA→CCA)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

AGATGACAGGAACTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACTACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGA  >  NC_000913/2056194‑2056463                                                                                                                                       |                                                                                                                                        aGATGACAGGAACTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACtgt                                                                                                                                    >  2:340525/1‑140 (MQ=255)  gATGACAGGAACTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACtgtg                                                                                                                                   <  1:53064/140‑1 (MQ=255)        aGGAACTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTc                                                                                                                             >  1:448478/1‑140 (MQ=255)           aaCTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGa                                                                                                                          >  2:468283/1‑140 (MQ=255)           aaCTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGa                                                                                                                          >  2:236194/1‑140 (MQ=255)               gggAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATaaa                                                                                                                      >  2:550156/1‑140 (MQ=255)                 gAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAAcc                                                                                                                    <  1:340525/140‑1 (MQ=255)                     tACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTg                                                                                                                >  2:916731/1‑140 (MQ=255)                        gTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATaaa                                                                                                                      >  2:739101/1‑131 (MQ=255)                        gTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATaaa                                                                                                                      <  1:739101/131‑1 (MQ=255)                          tACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATgg                                                                                                           <  2:448478/140‑1 (MQ=255)                          tACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATgg                                                                                                           <  1:468283/140‑1 (MQ=255)                                         cTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTcc                                                                                            <  2:951598/140‑1 (MQ=255)                                           tCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCg                                                                                          >  1:15352/1‑140 (MQ=255)                                           tCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCg                                                                                          >  1:478102/1‑140 (MQ=255)                                                ttAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAg                                                                                     <  2:515968/140‑1 (MQ=255)                                                     ttAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTACGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTaa                                                                                <  1:187473/140‑1 (MQ=255)                                                        aGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCg                                                                             >  2:150991/1‑140 (MQ=255)                                                             ggCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGttttt                                                                        >  1:621386/1‑140 (MQ=255)                                                                  tAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGaa                                                                   >  1:893926/1‑140 (MQ=255)                                                                    gCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAgg                                                                 <  1:916731/140‑1 (MQ=255)                                                                        ttGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCt                                                             <  2:167689/140‑1 (MQ=255)                                                                               ggAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCg                                                      <  1:424682/140‑1 (MQ=255)                                                                                     tGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATc                                                >  1:346137/1‑140 (MQ=255)                                                                                        aaCCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGc                                             >  2:841229/1‑140 (MQ=255)                                                                                                   cgcAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCAtt                                  <  2:234272/140‑1 (MQ=255)                                                                                                   cgcAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCAtt                                  >  1:234272/1‑140 (MQ=255)                                                                                                    gcAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTc                                 >  2:155816/1‑140 (MQ=255)                                                                                                     cAAGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCg                                <  2:893926/140‑1 (MQ=255)                                                                                                      aaGGATATCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCgg                               >  1:971113/1‑140 (MQ=255)                                                                                                          atatCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGcga                           >  1:125337/1‑140 (MQ=255)                                                                                                             tCGTTTCCGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGcgatcg                        >  1:580569/1‑140 (MQ=255)                                                                                                                   ccGCGTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTtgc                  >  2:941696/1‑140 (MQ=255)                                                                                                                    cgcgTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTtgct                 <  1:155850/140‑1 (MQ=255)                                                                                                                    cgcgTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTtgct                 >  1:226103/1‑140 (MQ=255)                                                                                                                    cgcgTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTtgct                 >  1:444580/1‑140 (MQ=255)                                                                                                                      cgTGCCATACGGGACACCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTtgctgc               <  2:621386/140‑1 (MQ=255)                                                                                                                                   acacCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGcga  <  2:580569/140‑1 (MQ=255)                                                                                                                                   acacCACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGcga  <  1:155816/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGATGACAGGAACTGGGAATTACGTTACTCAGCTTCTGCACTTCGTTTTAACTTAAGCCGGGCCGTAGCAATTGATATGGAAAGTGCAACCATTGCCGCGCAAGGATATCGTTTCCGCGTGCCATACGGGACACTACTGTGTGTTTCAGATAAACCGTTGCATGGCGAGATTAAACTTCCCGGCCAGGCTAACCGTTTTTATGAAGGCGCTATTTCCGAACATCTGCAAATTGGCATTCGGGCGATCGATTTGCTGCGCGCAGAAGGCGA  >  NC_000913/2056194‑2056463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑