BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,197	A→G	V3A (GTG→GCG)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,197	0	A	G	100.0%	117.5 / NA	34	V3A (GTG→GCG)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (14/20); total (14/20)

GATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCT  >  NC_000913/2177064‑2177333

gatgatGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACa                                                                                                                                    <  1:287094/140‑1 (MQ=255)

gatgatGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACa                                                                                                                                    <  2:462226/140‑1 (MQ=255)

gatgatGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACa                                                                                                                                    <  2:107879/140‑1 (MQ=255)

      gaCCGGCGCATGCAGGTTGGCAGCGGTTTCTCCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCa                                                                                                                              <  2:297066/140‑1 (MQ=255)

           gcgcATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATcc                                                                                                                         <  1:309535/140‑1 (MQ=255)

            cgcATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCt                                                                                                                        >  1:501235/1‑140 (MQ=255)

                 gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCgt                                                                                                                   >  2:873664/1‑140 (MQ=255)

                 gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCgt                                                                                                                   >  2:853099/1‑140 (MQ=255)

                 gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCgt                                                                                                                   <  1:645488/140‑1 (MQ=255)

                           aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                         >  1:536654/1‑140 (MQ=255)

                               gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                     >  2:442188/1‑140 (MQ=255)

                                  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                  <  1:4406/140‑1 (MQ=255)

                                   cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                 >  2:416692/1‑140 (MQ=255)

                                       caccatTTGCATCGTTTCGAGTTTGTGAATATTGAATGCCGAAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                             <  2:581121/140‑1 (MQ=255)

                                        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                            >  1:412403/1‑140 (MQ=255)

                                          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                          <  2:529529/140‑1 (MQ=255)

                                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                   >  1:782747/1‑140 (MQ=255)

                                                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                  <  1:416692/140‑1 (MQ=255)

                                                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                   >  1:525477/1‑138 (MQ=255)

                                                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                   <  2:525477/138‑1 (MQ=255)

                                                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                               >  2:161512/1‑140 (MQ=255)

                                                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                     <  1:442188/140‑1 (MQ=255)

                                                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                               <  2:285646/140‑1 (MQ=255)

                                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                           >  2:350933/1‑140 (MQ=255)

                                                                                gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                    >  2:280075/1‑140 (MQ=255)

                                                                                 aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaa                                                   <  2:501235/140‑1 (MQ=255)

                                                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                              <  2:616626/140‑1 (MQ=255)

                                                                                           ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAata                                         >  1:75527/1‑140 (MQ=255)

                                                                                                      tgCGTTGTTCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                              >  2:24314/1‑140 (MQ=255)

                                                                                                          ttgttCAGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                          <  1:161512/140‑1 (MQ=255)

                                                                                                                aGCATCTGCTTTGTCGATACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAATGACAATATGAAAATTATTCGAGGAGTGa                    >  1:112996/1‑140 (MQ=255)

                                                                                                                                aTACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc    <  1:873664/140‑1 (MQ=255)

                                                                                                                                 tACCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc   <  1:350933/140‑1 (MQ=255)

                                                                                                                                  aCCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt  <  1:853099/140‑1 (MQ=255)

                                                                                                                                  aCCGCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt  <  2:536654/140‑1 (MQ=255)

GATGATGACCGGCGCATGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCT  >  NC_000913/2177064‑2177333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`