Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,275,146 T→C V323A (GTC→GCC)  yejB → putative oligopeptide ABC transporter membrane subunit YejB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,275,1460TC100.0% 149.5 / NA 43V323A (GTC→GCC) yejBputative oligopeptide ABC transporter membrane subunit YejB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TTCCGCAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGTCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGA  >  NC_000913/2275013‑2275281
                                                                                                                                     |                                                                                                                                       
ttCCGCAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTcgcg                                                                                                                                   <  2:833517/140‑1 (MQ=255)
   cGCAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGa                                                                                                                                <  2:953866/140‑1 (MQ=255)
   cGCAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGa                                                                                                                                <  1:602841/140‑1 (MQ=255)
     cAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGAtt                                                                                                                              >  2:842977/1‑140 (MQ=255)
     cAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGAtt                                                                                                                              <  1:358107/140‑1 (MQ=255)
     cAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGAtt                                                                                                                              >  2:515521/1‑140 (MQ=255)
        cGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATc                                                                                                                           >  1:704227/1‑140 (MQ=255)
          ccATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCt                                                                                                                         >  1:556525/1‑140 (MQ=255)
                  ctgGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGAtt                                                                                                                              <  2:815024/127‑1 (MQ=255)
                  ctgGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGAtt                                                                                                                              >  1:815024/1‑127 (MQ=255)
                     gTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTgg                                                                                                              <  1:559315/140‑1 (MQ=255)
                        aTTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTAc                                                                                                           <  2:556525/140‑1 (MQ=255)
                         ttGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCCGTAATGTTTGGTAcc                                                                                                          <  1:540523/140‑1 (MQ=255)
                          tGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCt                                                                                                         >  2:332013/1‑140 (MQ=255)
                          tGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCt                                                                                                         >  1:462150/1‑140 (MQ=255)
                            ccGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTg                                                                                                       >  1:418312/1‑140 (MQ=255)
                                                  cAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGcc                                                                                 <  2:615401/140‑1 (MQ=255)
                                                    gCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCctg                                                                               <  1:242500/140‑1 (MQ=255)
                                                    gCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCctg                                                                               <  2:671646/140‑1 (MQ=255)
                                                     cATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCctgc                                                                              >  2:685466/1‑140 (MQ=255)
                                                       tGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCctgctg                                                                            <  2:418312/140‑1 (MQ=255)
                                                       tGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCctgctg                                                                            <  2:462150/140‑1 (MQ=255)
                                                               aCCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAatat                                                                    <  2:478683/140‑1 (MQ=255)
                                                                 cGGCTCGCTGCTGAGTGAGGTGATGTTTTCATTGAATGGTCTGGGCTTACTTGGCCACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTg                                                                  >  2:358419/1‑140 (MQ=255)
                                                                   gCTCGCTGCTGATTGCGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGCTTGGCCTGCTGCTGAATATTGTc                                                                >  1:501393/1‑140 (MQ=255)
                                                                    cTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCa                                                               <  1:685466/140‑1 (MQ=255)
                                                                    cTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCa                                                               <  1:332013/140‑1 (MQ=255)
                                                                          gctgATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGata                                                         <  1:842977/140‑1 (MQ=255)
                                                                                    gTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATAc                                               <  1:28471/140‑1 (MQ=255)
                                                                                    gTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATAc                                               <  2:501393/140‑1 (MQ=255)
                                                                                      gATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGc                                             <  2:704227/140‑1 (MQ=255)
                                                                                       aTGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCt                                            >  1:353066/1‑140 (MQ=255)
                                                                                                 tCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCcg                                  >  1:376036/1‑140 (MQ=255)
                                                                                                  cAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCcgc                                 >  2:687100/1‑140 (MQ=255)
                                                                                                  cAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCcgc                                 >  2:772033/1‑140 (MQ=255)
                                                                                                          tGGGCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGAt                         >  1:355067/1‑140 (MQ=255)
                                                                                                             gCTTACTGGGCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGAtttt                      >  2:678367/1‑140 (MQ=255)
                                                                                                                      gCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTg                     <  1:947623/132‑1 (MQ=255)
                                                                                                                      gCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTg                     >  2:947623/1‑132 (MQ=255)
                                                                                                                      gCTACGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCATGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGt             >  2:684527/1‑140 (MQ=255)
                                                                                                                          cGAAGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGTTAAt         >  1:40038/1‑140 (MQ=255)
                                                                                                                             aGCGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTc      <  2:355067/140‑1 (MQ=255)
                                                                                                                               cGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAgg                  >  2:460090/1‑126 (MQ=255)
                                                                                                                               cGACCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAgg                  <  1:460090/126‑1 (MQ=255)
                                                                                                                                 aCCGCCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGa  >  1:317165/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                       
TTCCGCAACGCCATGCTGCTGGTGATTGCCGGTTTTCCGGCGACGTTTATCAGCATGTTTTTTACCGGCTCGCTGCTGATTGAGGTGATGTTTTCACTCAATGGTCTGGGCTTACTGGGCTACGAAGCGACCGTCTCGCGCGATTATCCTGTAATGTTTGGTACCTTGTATATTTTCACCCTGATTGGCCTGCTGCTGAATATTGTCAGTGATATCAGCTATACGCTGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGA  >  NC_000913/2275013‑2275281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑