Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,385,750	A→G	intergenic (‑28/‑110)	yfbK ← / → yfbL	IPR002035/DUF3520 domain‑containing protein YfbK/putative peptidase YfbL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,385,750	0	A	G	100.0%	161.4 / NA	46	intergenic (‑28/‑110)	yfbK/yfbL	IPR002035/DUF3520 domain‑containing protein YfbK/putative peptidase YfbL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

GTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTAT  >  NC_000913/2385613‑2385885                                                                                                                                          |                                                                                                                                        gtgGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGcc                                                                                                                                       >  1:796258/1‑140 (MQ=255)  tgGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCa                                                                                                                                      >  2:441078/1‑140 (MQ=255)       gTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACAt                                                                                                                                 <  2:248431/140‑1 (MQ=255)       gTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACAt                                                                                                                                 <  2:796258/140‑1 (MQ=255)          cTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGAc                                                                                                                                   >  2:430244/1‑135 (MQ=255)          cTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGAc                                                                                                                                   <  1:430244/135‑1 (MQ=255)          cTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATaaa                                                                                                                              >  1:607798/1‑140 (MQ=255)            gggTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAat                                                                                                                            >  1:272460/1‑140 (MQ=255)             ggTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAata                                                                                                                           <  2:573201/140‑1 (MQ=255)              gttgttgttGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAatat                                                                                                                          <  2:655458/140‑1 (MQ=255)               ttgttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAAtata                                                                                                                         <  1:441078/140‑1 (MQ=255)                 gttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATAct                                                                                                                       >  1:436694/1‑140 (MQ=255)                 gttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATAct                                                                                                                       <  1:531590/140‑1 (MQ=255)                 gttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATAct                                                                                                                       <  2:588563/140‑1 (MQ=255)                 gttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATAct                                                                                                                       <  1:382152/140‑1 (MQ=255)                  ttgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATActc                                                                                                                      >  1:539925/1‑140 (MQ=255)                   tgttgCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATActct                                                                                                                     <  2:973088/140‑1 (MQ=255)                        cTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGta                                                                                                                <  2:272460/140‑1 (MQ=255)                           aCTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATaa                                                                                                             <  1:261388/140‑1 (MQ=255)                            cTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATActct                                                                                                                     <  1:615154/131‑1 (MQ=255)                            cTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATActct                                                                                                                     >  2:615154/1‑131 (MQ=255)                                ccTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTg                                                                                                        <  2:286412/140‑1 (MQ=255)                                   tATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTa                                                                                                     <  2:133945/140‑1 (MQ=255)                                       ctcAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTCCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCt                                                                                                 <  2:607798/140‑1 (MQ=255)                                               gCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTtaa                                                                                              <  2:817248/135‑1 (MQ=255)                                               gCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTtaa                                                                                              >  1:817248/1‑135 (MQ=255)                                               gCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATaaaa                                                                                         >  1:747261/1‑140 (MQ=255)                                                    ccACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACaa                                                                                    <  1:166104/140‑1 (MQ=255)                                                                  aaTCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCacg                                                                      >  1:349257/1‑138 (MQ=255)                                                                  aaTCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATa                                                                      >  1:832200/1‑140 (MQ=255)                                                                                tAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGc                                                                    <  2:702054/128‑1 (MQ=255)                                                                                tAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGc                                                                    >  1:702054/1‑128 (MQ=255)                                                                                tAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTc                                                        <  2:747261/140‑1 (MQ=255)                                                                                  aGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAaata                                                               >  1:608568/1‑131 (MQ=255)                                                                                  aGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAaata                                                               <  2:608568/131‑1 (MQ=255)                                                                                      aCATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCaacaac                                                  >  2:200776/1‑140 (MQ=255)                                                                                         tGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCa                                               >  2:778716/1‑140 (MQ=255)                                                                                          gATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCaa                                              <  1:778716/140‑1 (MQ=255)                                                                                           aTTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAAc                                             >  1:8668/1‑140 (MQ=255)                                                                                             tataTTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAAccc                                           >  1:968031/1‑140 (MQ=255)                                                                                                 tttttATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATta                                       <  1:85542/140‑1 (MQ=255)                                                                                                                          ttAAATAAACTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAaataat              >  2:614071/1‑140 (MQ=255)                                                                                                                                aaaCTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGc        <  2:968031/140‑1 (MQ=255)                                                                                                                                  aCTCAGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCtt      <  1:200776/140‑1 (MQ=255)                                                                                                                                      aGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTttat  <  2:539925/140‑1 (MQ=255)                                                                                                                                      aGGCGCCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTttat  <  2:455630/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAACTCAGGCACCAGACATAAAATATACTCTTTGTATAATCCTGCTAATCTTAATAAAATACAATATCGAAACCCATAGCAAATAATGATTCAACAACGCAACCCATTAATAATTTGCATATGAAAAAAATAATCTTTGCTTTTAT  >  NC_000913/2385613‑2385885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑