Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,447,796	A→G	D215D (GAT→GAC)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,447,796	0	A	G	100.0%	131.2 / NA	40	D215D (GAT→GAC)	prmB	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/30);  total (10/30)

CAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGC  >  NC_000913/2447662‑2447927                                                                                                                                       |                                                                                                                                    caAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTc                                                                                                                                <  1:946529/140‑1 (MQ=255)   aaTCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCg                                                                                                                              <  2:311468/140‑1 (MQ=255)     tCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCa                                                                                                                            <  1:499417/140‑1 (MQ=255)     tCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGACGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCa                                                                                                                            <  2:722549/140‑1 (MQ=255)      cAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCAt                                                                                                                           >  1:674531/1‑140 (MQ=255)            gCCATCACCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACAt                                                                                                                     >  1:7809/1‑140 (MQ=255)                tcatcaGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATAcgg                                                                                                                 <  1:27259/140‑1 (MQ=255)                 catcaGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTACGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATAcggc                                                                                                                <  1:214473/140‑1 (MQ=255)                       cAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTa                                                                                                          >  1:778721/1‑140 (MQ=255)                         aGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGt                                                                                                        <  2:37423/140‑1 (MQ=255)                          ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTg                                                                                                       >  1:434675/1‑140 (MQ=255)                          ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTAGTg                                                                                                       >  1:76034/1‑140 (MQ=255)                            tAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTtgtgga                                                                                                     >  1:190300/1‑138 (MQ=255)                              aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACaa                                                                                                   <  2:671187/140‑1 (MQ=255)                              aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTTGTGGCaa                                                                                                   >  2:502850/1‑140 (MQ=255)                              aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGCTAGTGACaa                                                                                                   <  1:502850/140‑1 (MQ=255)                               tCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGGCGGACATGTCTTCCGCATCGACATACGGCGGGGTtgtgggaaa                                                                                                  >  2:908244/1‑135 (MQ=255)                                          aCCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACt                                                                                       <  1:538293/140‑1 (MQ=255)                                           ccGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTg                                                                                      <  2:778721/140‑1 (MQ=255)                                                     gACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTTGTGGCAGGCAGGGCGGACTGCACtttttgc                                                                            >  2:530712/1‑136 (MQ=255)                                                          gTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTTGTGGCAAAAAGGGCGGACTGCACCTTTTGCCAGTc                                                                       >  2:21735/1‑140 (MQ=255)                                                            cAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTcgc                                                                     <  1:33297/140‑1 (MQ=255)                                                             aGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTcgcg                                                                    <  2:434675/140‑1 (MQ=255)                                                                ttCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGaa                                                                 <  2:701029/140‑1 (MQ=255)                                                                                 aGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCg                                                <  1:255672/140‑1 (MQ=255)                                                                                    tGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGaa                                             <  2:709653/140‑1 (MQ=255)                                                                                      ccAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATg                                           <  2:851853/140‑1 (MQ=255)                                                                                       cAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGa                                          <  1:908244/140‑1 (MQ=255)                                                                                       cAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGa                                          <  1:943930/140‑1 (MQ=255)                                                                                       cAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGa                                          <  1:679040/140‑1 (MQ=255)                                                                                         ggCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACg                                        <  1:485909/140‑1 (MQ=255)                                                                                          gCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGGTTGTGACAAACAGGGCGTGCTGCACCTTTTGCAAGTCGCGGGAAAGAACGGGAAGAAACGGGAAGACgg                                       >  2:403714/1‑139 (MQ=255)                                                                                           cccAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGtt                                      <  1:403714/140‑1 (MQ=255)                                                                                           cccAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGtt                                      <  2:386953/140‑1 (MQ=255)                                                                                                 tCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGAt                                <  1:962863/140‑1 (MQ=255)                                                                                                       tCGTGGCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGAcc                          <  1:21735/140‑1 (MQ=255)                                                                                                            gCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGtt                     <  2:76034/140‑1 (MQ=255)                                                                                                            gCGGTATTCGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGtt                     <  2:7809/140‑1 (MQ=255)                                                                                                                    cGTTTGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg             >  2:752970/1‑140 (MQ=255)                                                                                                                       ttGGCAGGTCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttc          <  2:191402/140‑1 (MQ=255)                                                                                                                               tCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGc  <  2:630706/140‑1 (MQ=255)                                                                                                                               tCGGACATGTCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGc  <  1:752970/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    CAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCACTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGC  >  NC_000913/2447662‑2447927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑