Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,492,338	A→G	F306S (TTC→TCC)	frc ←	formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,492,338	0	A	G	100.0%	245.3 / NA	69	F306S (TTC→TCC)	frc	formyl‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/36);  total (33/36)

CGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGGAT  >  NC_000913/2492203‑2492467                                                                                                                                        |                                                                                                                                  cgcAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAat                                                                                                                               >  1:589113/1‑140 (MQ=255)    aaaGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATAtg                                                                                                                            <  2:409523/140‑1 (MQ=255)      agagGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATAtgtg                                                                                                                          >  1:522819/1‑140 (MQ=255)       gagGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTgg                                                                                                                         <  2:18189/140‑1 (MQ=255)             tCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCg                                                                                                                   <  1:808761/140‑1 (MQ=255)              cAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGt                                                                                                                  >  1:986181/1‑140 (MQ=255)                aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGc                                                                                                                <  2:265561/140‑1 (MQ=255)                aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGc                                                                                                                >  2:529284/1‑140 (MQ=255)                aGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGc                                                                                                                <  1:529284/140‑1 (MQ=255)                  tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCAt                                                                                                              >  2:841084/1‑140 (MQ=255)                  tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCAt                                                                                                              >  1:541690/1‑140 (MQ=255)                  tGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCAt                                                                                                              <  1:476145/140‑1 (MQ=255)                    aaaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATgg                                                                                                            >  1:8788/1‑140 (MQ=255)                    aaaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATgg                                                                                                            <  2:522819/140‑1 (MQ=255)                    aaaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATgg                                                                                                            >  1:184113/1‑140 (MQ=255)                     aaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATggg                                                                                                           <  2:589113/140‑1 (MQ=255)                     aaTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATggg                                                                                                           <  2:792662/140‑1 (MQ=255)                      aTTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGc                                                                                                          >  1:604925/1‑140 (MQ=255)                       tttctttcATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGct                                                                                                         <  1:54970/140‑1 (MQ=255)                        ttctttcATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGctg                                                                                                        <  1:252023/140‑1 (MQ=255)                        ttctttcATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGctg                                                                                                        <  1:919178/140‑1 (MQ=255)                            ttcATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGctgtact                                                                                                    >  1:697199/1‑140 (MQ=255)                                tACTTAAAACCGGTGCACAATGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTAt                                                                                                <  1:68618/140‑1 (MQ=255)                                     aaaaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCgg                                                                                           <  1:665086/140‑1 (MQ=255)                                     aaaaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCgg                                                                                           <  2:604925/140‑1 (MQ=255)                                       aaCCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGAt                                                                                         <  2:592495/140‑1 (MQ=255)                                            gTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTa                                                                                    >  2:228317/1‑140 (MQ=255)                                            gTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTa                                                                                    >  1:739543/1‑140 (MQ=255)                                            gTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTa                                                                                    >  1:239460/1‑140 (MQ=255)                                             tGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTaa                                                                                   >  2:469112/1‑140 (MQ=255)                                             tGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTaa                                                                                   >  1:110208/1‑140 (MQ=255)                                             tGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTaa                                                                                   >  1:690547/1‑140 (MQ=255)                                              gCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAAt                                                                                  <  2:145593/140‑1 (MQ=255)                                                       atatCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTgg                                                                         >  1:514354/1‑140 (MQ=255)                                                              aCTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCg                                                                  >  1:979037/1‑140 (MQ=255)                                                              aCTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCg                                                                  <  2:514354/140‑1 (MQ=255)                                                                  aGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATgg                                                              >  2:687149/1‑140 (MQ=255)                                                                         tAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACa                                                       <  2:828239/140‑1 (MQ=255)                                                                            gCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGgt                                                    >  1:745908/1‑140 (MQ=255)                                                                            gCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGgt                                                    >  2:682684/1‑140 (MQ=255)                                                                              cACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGgtgt                                                  >  1:758391/1‑140 (MQ=255)                                                                                ccGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGttt                                                >  1:33948/1‑140 (MQ=255)                                                                                   cTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTcc                                             >  1:676226/1‑140 (MQ=255)                                                                                        tGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGtt                                        <  2:33948/140‑1 (MQ=255)                                                                                         gTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGttt                                       <  2:697199/140‑1 (MQ=255)                                                                                         gTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGttt                                       <  2:421229/140‑1 (MQ=255)                                                                                             aTCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCt                                   >  2:168526/1‑140 (MQ=255)                                                                                              tCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTc                                  <  2:184113/140‑1 (MQ=255)                                                                                                   aGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTccc                                            <  2:464504/125‑1 (MQ=255)                                                                                                   aGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTccc                                            >  1:464504/1‑125 (MQ=255)                                                                                                   aGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGaa                             <  1:687149/140‑1 (MQ=255)                                                                                                    gTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAAt                            >  2:205338/1‑140 (MQ=255)                                                                                                     tGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATa                           <  1:205338/140‑1 (MQ=255)                                                                                                     tGACAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGGGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCGAGTTTTGCTCCTGAATa                           >  2:288830/1‑140 (MQ=255)                                                                                                       aCAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCt                                <  1:844160/133‑1 (MQ=255)                                                                                                       aCAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCt                                >  2:844160/1‑133 (MQ=255)                                                                                                       aCAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGt                         >  2:366429/1‑140 (MQ=255)                                                                                                       aCAGTGTATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGt                         <  1:366429/140‑1 (MQ=255)                                                                                                          gtgtATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGt                         >  1:115893/1‑137 (MQ=255)                                                                                                          gtgtATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGt                         <  2:115893/137‑1 (MQ=255)                                                                                                          gtgtATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGaa                      <  2:986181/140‑1 (MQ=255)                                                                                                            gtATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGaaat                    >  2:468537/1‑140 (MQ=255)                                                                                                            gtATTTTTCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGaaat                    <  1:168526/140‑1 (MQ=255)                                                                                                                 tttCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAaataaata               <  2:739543/140‑1 (MQ=255)                                                                                                                  ttCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAatat              <  2:690547/140‑1 (MQ=255)                                                                                                                   tCGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTTCTGAATAGTGAAATAAAtata             <  2:588189/140‑1 (MQ=255)                                                                                                                    cGATTTCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAg            <  2:239460/140‑1 (MQ=255)                                                                                                                       tttCAGCAAAAATATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCg         >  1:547400/1‑140 (MQ=255)                                                                                                                              aaaaaTATCGGAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGGAt  <  2:154984/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CGCAAAGAGGGATCAAGTGAAATTTCTTTCATACTTAAAACCGGTGCACAAGGAATATCAAACTGAGTCAAATAGGCCACCGCTTCATGTTTATCAATAGTGACAGTGTATTTTTCGATTTCAGCAAAAATATCGAAAATATGTGGCTGTCGTGCATGGGCTGTACTGTATGCCGGATCGGTAATCCATTCTGGTTTGCCGATGGCTTTACAGGTGTTTTCCCAGTTTTGCTCCTGAATAGTGAAATAAATATAGGCGTTAGGAT  >  NC_000913/2492203‑2492467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑