Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,497,609	T→C	V229V (GTA→GTG)	alaC ←	glutamate‑‑pyruvate aminotransferase AlaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,497,609	0	T	C	100.0%	157.5 / NA	45	V229V (GTA→GTG)	alaC	glutamate‑‑pyruvate aminotransferase AlaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/19);  total (26/19)

CTTTTAATACGAGCCAGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGTACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGC  >  NC_000913/2497484‑2497743                                                                                                                              |                                                                                                                                       cTTTTAATACGAGCCAGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGa                                                                                                                          >  1:925882/1‑140 (MQ=255)     tAATACGAGCCAGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGgc                                                                                                                      >  1:34687/1‑140 (MQ=255)              ccAGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAg                                                                                                             <  1:197100/140‑1 (MQ=255)                aGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGcc                                                                                                           >  2:943712/1‑140 (MQ=255)                 gggCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCa                                                                                                          <  2:410870/140‑1 (MQ=255)                  ggCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCAt                                                                                                         >  2:298484/1‑140 (MQ=255)                    cgcTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCTTGTTGTAGCCTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCg                                                                                                       <  2:880904/140‑1 (MQ=255)                      cTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTa                                                                                                     >  1:155267/1‑140 (MQ=255)                       tGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAg                                                                                                    >  1:369125/1‑140 (MQ=255)                       tGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAg                                                                                                    <  2:773182/140‑1 (MQ=255)                       tGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAg                                                                                                    >  2:655394/1‑140 (MQ=255)                         aCCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGAc                                                                                                  <  2:155267/140‑1 (MQ=255)                         aCCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGAc                                                                                                  >  2:736447/1‑140 (MQ=255)                           cAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGa                                                                                                >  2:514211/1‑140 (MQ=255)                              cGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatgt                                                                                             <  2:568783/140‑3 (MQ=255)                              cGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatat                                                                                             >  2:28985/1‑140 (MQ=255)                              cGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatat                                                                                             >  2:150107/1‑140 (MQ=255)                              cGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatat                                                                                             <  1:565562/140‑1 (MQ=255)                                  ttgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGc                                                                                         <  2:369125/140‑1 (MQ=255)                                   ttgtgCCAACCATAAAGCCGATGCGACAGCCCGCAATGTTGTAGCGTTTCGACAGCGTAAAGACCTCGGCTGGCACATCGCGTGCACCCGGTACCTGCATAATAGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCa                                                                                        <  2:11012/137‑1 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatat                                                                                             >  2:365801/1‑135 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGatat                                                                                             <  1:365801/135‑1 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCa                                                                                        <  1:492627/140‑1 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCa                                                                                        >  2:22536/1‑140 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCa                                                                                        <  1:21526/140‑1 (MQ=255)                                   tgttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCa                                                                                        <  1:22536/140‑1 (MQ=255)                                     ttgCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATa                                                                                      <  1:298484/140‑1 (MQ=255)                                         cAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGcc                                                                                  <  2:925882/140‑1 (MQ=255)                                          aaCCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCa                                                                                 <  1:28985/140‑1 (MQ=255)                                                  aGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATgg                                                                         <  1:736447/140‑1 (MQ=255)                                                       aTACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGaccac                                                                    <  1:722792/140‑1 (MQ=255)                                                                          tgtAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTc                                                 >  1:463424/1‑140 (MQ=255)                                                                               cTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAg                                            >  2:927206/1‑140 (MQ=255)                                                                                 tttCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAgcg                                          >  2:199119/1‑140 (MQ=255)                                                                                   tCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGcc                                        >  2:242084/1‑140 (MQ=255)                                                                                   tCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGcc                                        >  2:63720/1‑140 (MQ=255)                                                                                   tCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGcc                                        >  1:186047/1‑140 (MQ=255)                                                                                          cGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACtt                                 >  1:613864/1‑140 (MQ=255)                                                                                                    tCGACTGCCACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCgaagaa                       >  1:834746/1‑140 (MQ=255)                                                                                                           ccACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGt                >  2:17527/1‑140 (MQ=255)                                                                                                           ccACATCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGt                >  1:694508/1‑140 (MQ=255)                                                                                                                tCGCGTGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCAc           >  1:436752/1‑140 (MQ=255)                                                                                                                     tGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCa                  >  2:375593/1‑128 (MQ=255)                                                                                                                     tGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCa                  <  1:375593/128‑1 (MQ=255)                                                                                                                     tGCACCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCAtt      >  1:512411/1‑140 (MQ=255)                                                                                                                        aCCCGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTgcg   <  1:150107/140‑1 (MQ=255)                                                                                                                         cccGGCACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTgcgc  >  2:293330/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       CTTTTAATACGAGCCAGGGCGCTGACCAGCGTTTTGTTGCCAACCATAAAGCCGATACGCCAGCCCGCCATGTTGTAGCTTTTCGACAGCGTAAAGAACTCGACTGCCACATCGCGTGCACCCGGTACCTGCATAATTGACGGCGCTTTCCAGCCATCGTAGACGATATCGGCATAGGCCAGGTCATGGACCACCAGCACATCGTAGCGTTTCGCCAGCGCCACTACTTTTTCGAAGAACTCCAGTTCCACGCATTGCGC  >  NC_000913/2497484‑2497743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑