Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	432,249	A→G	intergenic (‑236/+63)	tsx ← / ← yajI	nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,249	0	A	G	100.0%	193.2 / NA	55	intergenic (‑236/+63)	tsx/yajI	nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/24);  total (31/24)

TGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAACTGT  >  NC_000913/432112‑432384                                                                                                                                          |                                                                                                                                        tGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGATCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGc                                                                                                                                       >  1:449444/1‑140 (MQ=255)  gCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCa                                                                                                                                      <  2:314935/140‑1 (MQ=255)                  ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACg                                                                                                                      >  2:602364/1‑140 (MQ=255)                  ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACg                                                                                                                      <  1:609181/140‑1 (MQ=255)                       ttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGaa                                                                                                                 <  2:172069/140‑1 (MQ=255)                                cTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGc                                                                                                        >  2:947960/1‑140 (MQ=255)                                   tCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCggg                                                                                                     >  2:381049/1‑140 (MQ=255)                                   tCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCggg                                                                                                     >  1:252068/1‑140 (MQ=255)                                        tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                                                                >  2:695955/1‑140 (MQ=255)                                        tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                                                                >  1:249665/1‑140 (MQ=255)                                        tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                                                                >  1:275347/1‑140 (MQ=255)                                        tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                                                                <  1:894273/140‑1 (MQ=255)                                          cGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGcaca                                                                                              >  2:144857/1‑140 (MQ=255)                                             aaTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGTAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACaaaa                                                                                           >  1:527717/1‑140 (MQ=255)                                             aaTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACaaaa                                                                                           >  1:965984/1‑140 (MQ=255)                                             aaTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACaaaa                                                                                           >  2:260770/1‑140 (MQ=255)                                               tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                                                         >  1:524481/1‑140 (MQ=255)                                               tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                                                         >  1:659776/1‑140 (MQ=255)                                                cGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCg                                                                                        <  1:695955/140‑1 (MQ=255)                                                  aTTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGTAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTc                                                                                      <  2:527717/140‑1 (MQ=255)                                                       gTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGaa                                                                                 <  1:905176/140‑1 (MQ=255)                                                         tCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAAt                                                                               >  2:696178/1‑140 (MQ=255)                                                              tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                                                          <  2:659776/140‑1 (MQ=255)                                                              tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATAAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                                                          <  2:602876/140‑1 (MQ=255)                                                                tACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTa                                                                        >  2:530852/1‑140 (MQ=255)                                                                tACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATAAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCg                                                                                        >  1:251898/1‑124 (MQ=255)                                                                tACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATAAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCg                                                                                        <  2:251898/124‑1 (MQ=255)                                                                      caTTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCAt                                                                  <  1:602364/140‑1 (MQ=255)                                                                       aTTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATa                                                                 >  2:612087/1‑140 (MQ=255)                                                                        ttCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATaa                                                                <  2:72676/140‑1 (MQ=255)                                                                        ttCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATaa                                                                <  1:947960/140‑1 (MQ=255)                                                                         tCGGGCGGGGATTGTACTTACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                                                               >  1:928686/1‑140 (MQ=255)                                                                         tCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                                                               <  2:797165/140‑1 (MQ=255)                                                                         tCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                                                               <  2:524481/140‑1 (MQ=255)                                                                                        aCTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTg                                                <  1:745054/140‑1 (MQ=255)                                                                                              tttCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGAt                                             <  1:121541/137‑1 (MQ=255)                                                                                              tttCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGAt                                             >  2:121541/1‑137 (MQ=255)                                                                                              tttCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACg                                          >  1:969251/1‑140 (MQ=255)                                                                                              tttCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACACACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACg                                          >  2:72122/1‑140 (MQ=255)                                                                                                tCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTTACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTa                                        <  2:449444/140‑1 (MQ=255)                                                                                                tCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTa                                        <  1:161868/140‑1 (MQ=255)                                                                                                 cGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTAc                                       >  2:492273/1‑140 (MQ=255)                                                                                                 cGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTAc                                       >  1:116752/1‑140 (MQ=255)                                                                                                      ttgttgCAAACTTTTACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGc                                  <  1:407856/140‑1 (MQ=255)                                                                                                                   ttACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATc                        >  1:506295/1‑137 (MQ=255)                                                                                                                   ttACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATc                        <  2:506295/137‑1 (MQ=255)                                                                                                                   ttACTATCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCcac                     >  1:122301/1‑140 (MQ=255)                                                                                                                        aTCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGaaa                <  2:79847/140‑1 (MQ=255)                                                                                                                         tCAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAAt               >  2:236136/1‑140 (MQ=255)                                                                                                                          cAATCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATc              <  1:144857/140‑1 (MQ=255)                                                                                                                           aaTCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATcc             >  2:905492/1‑140 (MQ=255)                                                                                                                             tCCAGGTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATcccc           >  1:662473/1‑140 (MQ=255)                                                                                                                                 ggTGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTa       >  2:269655/1‑140 (MQ=255)                                                                                                                                   tGATGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAAc     >  2:476737/1‑140 (MQ=255)                                                                                                                                      tGTAGGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAACTGt  <  2:252068/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAACTGT  >  NC_000913/432112‑432384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑