Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,771,340	C→T	H65Y (CAC→TAC)	yfjT →	CP4‑57 prophage; uncharacterized protein YfjT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,771,340	0	C	T	100.0%	205.8 / NA	60	H65Y (CAC→TAC)	yfjT	CP4‑57 prophage; uncharacterized protein YfjT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/32);  total (28/32)

AGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGCACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTAACGGAGATAATGCC  >  NC_000913/2771207‑2771474                                                                                                                                      |                                                                                                                                       aGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCgg                                                                                                                                  <  1:418368/140‑1 (MQ=255) aGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCgg                                                                                                                                  <  2:223621/140‑1 (MQ=255)    ctctGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGttt                                                                                                                               >  1:474051/1‑140 (MQ=255)     tctGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGtttt                                                                                                                              >  1:986583/1‑140 (MQ=255)             ccccGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATcc                                                                                                                      >  2:730253/1‑140 (MQ=255)              cccGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCt                                                                                                                     >  2:655574/1‑140 (MQ=255)                 ggACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGc                                                                                                                  >  2:766490/1‑140 (MQ=255)                 ggACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGc                                                                                                                  >  2:193167/1‑140 (MQ=255)                             cAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAAc                                                                                                      >  1:872144/1‑140 (MQ=255)                                gggTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGt                                                                                                   <  2:696144/140‑1 (MQ=255)                                   tACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAg                                                                                                >  2:863604/1‑140 (MQ=255)                                     ctgTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCt                                                                                              <  1:220075/138‑1 (MQ=255)                                     cGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCt                                                                                              <  2:57668/140‑1 (MQ=255)                                     cGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCt                                                                                              <  2:305488/140‑1 (MQ=255)                                        tCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGc                                                                                           <  2:474051/140‑1 (MQ=255)                                          aGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCat                                                                                         >  1:527508/1‑140 (MQ=255)                                          aGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCat                                                                                         >  2:675324/1‑140 (MQ=255)                                          aGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCat                                                                                         <  1:766490/140‑1 (MQ=255)                                          aGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCat                                                                                         >  2:11098/1‑140 (MQ=255)                                                      aaTACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTAcc                                                                             >  1:779045/1‑140 (MQ=255)                                                       aTACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCg                                                                            >  2:283648/1‑140 (MQ=255)                                                             tgtgACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCAtgatga                                                                                    >  1:923775/1‑126 (MQ=255)                                                             tgtgACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCAtgatga                                                                                    <  2:923775/126‑1 (MQ=255)                                                             tgtgACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATca                                                                      >  2:148226/1‑140 (MQ=255)                                                               tgACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATca                                                                      >  2:194/1‑138 (MQ=255)                                                               tgACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATca                                                                      <  1:194/138‑1 (MQ=255)                                                                gACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAg                                                                   <  1:148226/140‑1 (MQ=255)                                                                gACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAg                                                                   >  1:631707/1‑140 (MQ=255)                                                                gACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAg                                                                   >  1:620527/1‑140 (MQ=255)                                                                 aCGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAgt                                                                  <  2:986583/140‑1 (MQ=255)                                                                  cGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAgtg                                                                 >  1:793310/1‑140 (MQ=255)                                                                   ggATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAgtgg                                                                <  2:793310/140‑1 (MQ=255)                                                                     aTGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAgtggtg                                                              >  2:424854/1‑140 (MQ=255)                                                                       gCCAGGGGAAAGATGTGTACCAACAACTGCACGGGAAATCCGGACTAGAACGGTACTTCCCAGCACTGGGGTTTTCTGATCCTCGCGGGGGATAAAACGGTCTGCTCGCATGAGGACAGTACCGGCATCACAGTGGTGAt                                                            <  2:258409/140‑1 (MQ=255)                                                                       gCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCgg                                                                           >  2:854936/1‑125 (MQ=255)                                                                       gCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCgg                                                                           <  1:854936/125‑1 (MQ=255)                                                                       gCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGAt                                                            <  2:631707/140‑1 (MQ=255)                                                                       gCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGAt                                                            <  1:616111/140‑1 (MQ=255)                                                                          aGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGa                                                         <  2:527508/140‑1 (MQ=255)                                                                            cGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGAcc                                                       <  1:863604/140‑1 (MQ=255)                                                                            cGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGAcc                                                       <  2:582937/140‑1 (MQ=255)                                                                               aaaGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATa                                                    <  1:675324/140‑1 (MQ=255)                                                                                   aTGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCa                                                <  1:193167/140‑1 (MQ=255)                                                                                       ttACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAatat                                            >  1:156045/1‑140 (MQ=255)                                                                                          ccATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGcc                                         <  1:283648/140‑1 (MQ=255)                                                                                           cATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCa                                        <  2:872144/140‑1 (MQ=255)                                                                                           cATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCa                                        <  2:365193/140‑1 (MQ=255)                                                                                            aTCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCAt                                       <  2:779045/140‑1 (MQ=255)                                                                                             tCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCAtt                                      >  1:602623/1‑140 (MQ=255)                                                                                                aCTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTccc                                   >  2:380068/1‑140 (MQ=255)                                                                                                 cTGCACGGGAAATCCGGACCAGAACGGTATCTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCt                                  <  2:602623/140‑1 (MQ=255)                                                                                                  tGCACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTc                                 <  1:380068/140‑1 (MQ=255)                                                                                                    cACGGGAAATCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCaa                               >  1:569243/1‑140 (MQ=255)                                                                                                            aTCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCAt                                       <  2:696192/124‑1 (MQ=255)                                                                                                            aTCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCAt                                       >  1:696192/1‑124 (MQ=255)                                                                                                            aTCCGGACCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTtggc                       <  1:11098/140‑1 (MQ=255)                                                                                                                  aCCAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGt                 >  1:278855/1‑140 (MQ=255)                                                                                                                    cAGAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTaa               <  1:655574/140‑1 (MQ=255)                                                                                                                      gAACGGTATTTACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTAACg             <  2:278855/140‑1 (MQ=255)                                                                                                                                tACCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTAACGGAGATAATGc   <  1:92518/140‑1 (MQ=255)                                                                                                                                 aCCAGTACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTAACGGAGATAATGcc  >  1:576756/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       AGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAACGGTATTTACCAGCACTCGGTTTTTCTGATCCTCGCCGGGGATAAAACGGTCAGCTCGCATGATGACAGTACCGGCATCACAGTGGTGATGGACCATAAGCAATATGCCATTCCCTCAACCCTTGGCTGGCGTAACGGAGATAATGCC  >  NC_000913/2771207‑2771474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑