Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,105	T→C	intergenic (+90/‑148)	gabT → / → gabP	4‑aminobutyrate aminotransferase GabT/4‑aminobutyrate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,105	0	T	C	100.0%	151.7 / NA	44	intergenic (+90/‑148)	gabT/gabP	4‑aminobutyrate aminotransferase GabT/4‑aminobutyrate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/23);  total (21/23)

TGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACAC  >  NC_000913/2793975‑2794235                                                                                                                                   |                                                                                                                                   tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTg                                                                                                                           <  2:896725/140‑1 (MQ=255) tGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTg                                                                                                                           >  2:229415/1‑140 (MQ=255)    agaTCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAAc                                                                                                                        <  2:339107/140‑1 (MQ=255)       tcatcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAgg                                                                                                                     <  1:444326/140‑1 (MQ=255)        catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGga                                                                                                                    <  1:461149/140‑1 (MQ=255)        catcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGga                                                                                                                    >  1:630199/1‑140 (MQ=255)         atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGgaa                                                                                                                   <  1:926073/140‑1 (MQ=255)         atcaGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGgaa                                                                                                                   <  1:841644/140‑1 (MQ=255)           caGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGgaaga                                                                                                                 <  1:301021/140‑1 (MQ=255)                  tgtTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTAt                                                                                                          >  1:552888/1‑140 (MQ=255)                  tgtTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTAt                                                                                                          >  1:678133/1‑140 (MQ=255)                       tgatgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTgg                                                                                                     <  1:229415/140‑1 (MQ=255)                       tgatgaGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTgg                                                                                                     <  2:678133/140‑1 (MQ=255)                            aGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCggg                                                                                                >  1:806955/1‑140 (MQ=255)                              gCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTgg                                                                                                     >  2:902170/1‑133 (MQ=255)                              gCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTgg                                                                                                     <  1:902170/133‑1 (MQ=255)                              gCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTa                                                                                              >  1:708357/1‑140 (MQ=255)                                 aaGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAgg                                                                                           <  2:135225/140‑1 (MQ=255)                                            ccgcTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGctc                                                                                >  1:46571/1‑140 (MQ=255)                                             cgcTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCGGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGctct                                                                               <  2:874625/140‑1 (MQ=255)                                                 ccTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCgg                                                                           <  2:708357/140‑1 (MQ=255)                                                 ccTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCgg                                                                           <  1:319041/140‑1 (MQ=255)                                                          gAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGaa                                                                  >  1:269099/1‑140 (MQ=255)                                                           aGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAAt                                                                 >  2:319714/1‑140 (MQ=255)                                                            ggCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATc                                                                >  1:885117/1‑140 (MQ=255)                                                            ggCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATc                                                                >  1:393185/1‑140 (MQ=255)                                                                 cGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCaaaaa                                                                     >  1:800596/1‑130 (MQ=255)                                                                 cGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCaaaaa                                                                     <  2:800596/130‑1 (MQ=255)                                                                 cGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATaa                                                           <  2:46571/140‑1 (MQ=255)                                                                  gCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAAc                                                          <  2:321926/140‑1 (MQ=255)                                                                      cgcgTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAAtt                                                      >  2:444700/1‑140 (MQ=255)                                                                       gcgTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATta                                                     <  1:265223/140‑1 (MQ=255)                                                                                   gCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGAccc                                         >  1:383178/1‑140 (MQ=255)                                                                                                     cGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCggg                       <  1:444700/140‑1 (MQ=255)                                                                                                     cGGATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCggg                       <  2:704081/140‑1 (MQ=255)                                                                                                       gATAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgc                     >  2:345003/1‑140 (MQ=255)                                                                                                        aTAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgcg                    <  2:806955/140‑1 (MQ=255)                                                                                                        aTAAGGCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGgcg                    >  1:143774/1‑140 (MQ=255)                                                                                                            ggCGTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGctct                >  1:407064/1‑140 (MQ=255)                                                                                                               gTTTACGCCGCATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTccc             <  2:269099/140‑1 (MQ=255)                                                                                                                       cgcATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGaca     >  2:766595/1‑140 (MQ=255)                                                                                                                        gcATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGct                  <  2:819757/126‑1 (MQ=255)                                                                                                                        gcATCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGct                  >  1:819757/1‑126 (MQ=255)                                                                                                                          aTCCGACAACCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGacacac  >  2:474907/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   TGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAAGAAATCTATGTTGGCCGGGTAAGGCGGAGCCGCTCTCCGGCAAAAAGAATCAATAACAATTATACGCGTGACCCGGCGCGGGAAATGTCGGGGCGCTCTCCCAAGTGACACAC  >  NC_000913/2793975‑2794235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑