Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,815,090	A→G	R450R (CGT→CGC)	gshA ←	glutamate‑‑cysteine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,815,090	0	A	G	100.0%	214.7 / NA	61	R450R (CGT→CGC)	gshA	glutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

AGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGATCTTTAC  >  NC_000913/2814955‑2815223                                                                                                                                        |                                                                                                                                      aGACGCCTCGCGCTCGGCTACAAAATCCTCTTCTCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCggg                                                                                                                                   <  1:885176/140‑1 (MQ=255)    cGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCag                                                                                                                                <  1:724467/140‑1 (MQ=255)     gCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCaga                                                                                                                               <  2:158223/140‑1 (MQ=255)     gCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCaga                                                                                                                               <  1:337041/140‑1 (MQ=255)        tCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCa                                                                                                                                 <  2:540930/135‑1 (MQ=255)        tCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCa                                                                                                                                 >  1:540930/1‑135 (MQ=255)         cgcgCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGaaa                                                                                                                           <  1:134570/140‑1 (MQ=255)             cTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCa                                                                                                                       >  2:485674/1‑140 (MQ=255)               cGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGa                                                                                                                     <  1:931881/140‑1 (MQ=255)               cGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGa                                                                                                                     >  2:743733/1‑140 (MQ=255)                 gCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATc                                                                                                                   >  2:520503/1‑140 (MQ=255)                          tCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATc                                                                                                          >  1:96290/1‑140 (MQ=255)                          tCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATc                                                                                                          >  2:409382/1‑140 (MQ=255)                              ctTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAg                                                                                                      <  1:526245/140‑1 (MQ=255)                               ttCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGc                                                                                                     >  1:299381/1‑140 (MQ=255)                               ttCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGc                                                                                                     >  1:147675/1‑140 (MQ=255)                               ttCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGc                                                                                                     >  2:422204/1‑140 (MQ=255)                                 cgcgCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAg                                                                                                   <  2:147675/140‑1 (MQ=255)                                 cgcgCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAg                                                                                                   <  1:409382/140‑1 (MQ=255)                                   cgcAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGc                                                                                                 <  1:520503/140‑1 (MQ=255)                                   cgcAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGc                                                                                                 <  1:728766/140‑1 (MQ=255)                                      aGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAAc                                                                                              >  1:727495/1‑140 (MQ=255)                                          tttCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGt                                                                                          <  2:96290/140‑1 (MQ=255)                                             ccAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtca                                                                                       >  1:963661/1‑140 (MQ=255)                                              cAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcat                                                                                      <  1:743733/140‑1 (MQ=255)                                                 cGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcac                                                                                   <  1:180930/140‑1 (MQ=255)                                                 cGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcac                                                                                   <  2:90230/140‑1 (MQ=255)                                                  ggCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcaca                                                                                  >  1:874881/1‑140 (MQ=255)                                                  ggCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcaca                                                                                  <  2:874881/140‑1 (MQ=255)                                                  ggCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcaca                                                                                  <  1:422204/140‑1 (MQ=255)                                                         cACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTc                                                                           <  2:727495/140‑1 (MQ=255)                                                            gcagcaGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGa                                                                        <  2:963661/140‑1 (MQ=255)                                                                 agATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGc                                                                   >  2:345552/1‑140 (MQ=255)                                                                            ggCTTCTGCAGATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGt                                                        <  1:512080/140‑1 (MQ=255)                                                                             gCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGtt                                                       >  2:929945/1‑140 (MQ=255)                                                                               ttCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTcgcc                                                             <  1:419459/132‑1 (MQ=255)                                                                               ttCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTcgcc                                                             >  2:419459/1‑132 (MQ=255)                                                                               ttCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTaa                                                     >  2:188008/1‑140 (MQ=255)                                                                                 cTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATa                                                   >  1:934560/1‑140 (MQ=255)                                                                                  tGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTTTGATACGCTTCGCCGCCGTTAATAc                                                  >  1:154176/1‑140 (MQ=255)                                                                                  tGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATAc                                                  >  2:939079/1‑140 (MQ=255)                                                                                  tGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATAc                                                  >  2:561651/1‑140 (MQ=255)                                                                                  tGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATAc                                                  >  2:781345/1‑140 (MQ=255)                                                                                            ttGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGt                                        >  2:255417/1‑140 (MQ=255)                                                                                             tGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGtt                                       >  2:794649/1‑140 (MQ=255)                                                                                                   ttCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTTTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGcgcg                                 <  2:154176/140‑1 (MQ=255)                                                                                                   ttCCGCCAATACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGcgcg                                 <  2:934560/140‑1 (MQ=255)                                                                                                            tACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTc                        >  1:42528/1‑140 (MQ=255)                                                                                                            tACCAGTATCAATCATAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTc                        >  1:627607/1‑140 (MQ=255)                                                                                                                   atcaatcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGAc                               >  1:749638/1‑126 (MQ=255)                                                                                                                   atcaatcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGAc                               <  2:749638/126‑1 (MQ=255)                                                                                                                      aatcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGa              >  1:718657/1‑140 (MQ=255)                                                                                                                       atcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGAc                               <  1:779086/122‑1 (MQ=255)                                                                                                                       atcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGAc                               >  2:779086/1‑122 (MQ=255)                                                                                                                        tcaTAGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAAc            <  1:794649/140‑1 (MQ=255)                                                                                                                            aGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGAt        >  1:146414/1‑140 (MQ=255)                                                                                                                            aGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGAt        >  2:587322/1‑140 (MQ=255)                                                                                                                            aGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGAt        >  1:92707/1‑140 (MQ=255)                                                                                                                            aGACCTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGAt        <  1:929945/140‑1 (MQ=255)                                                                                                                                cTTAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGATCttt    >  1:662485/1‑140 (MQ=255)                                                                                                                                  tAAGATGCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCCCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGATCTTTAc  >  1:643803/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGATTACGGTAGGCTTCTGCAAATGCTTTGCCTGTTCCGCCAATACCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGCGACGCGTTTCAGATCGCGGAACAGATCTTTAC  >  NC_000913/2814955‑2815223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑