Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,499	T→C	P117P (CCA→CCG)	hycB ←	formate hydrogenlyase subunit HycB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,499	0	T	C	100.0%	100.6 / NA	31	P117P (CCA→CCG)	hycB	formate hydrogenlyase subunit HycB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/17);  total (14/17)

GCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCC  >  NC_000913/2849362‑2849633                                                                                                                                          |                                                                                                                                       gCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCcggcgg                                                                                                                                      <  2:816822/140‑1 (MQ=255)  cTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCggcggc                                                                                                                                     <  2:679141/140‑1 (MQ=255)    ttAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGgcgc                                                                                                                                   <  2:318216/140‑1 (MQ=255)          gggCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCgt                                                                                                                             <  1:563087/140‑2 (MQ=255)          gggCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCgg                                                                                                                             >  1:350767/1‑140 (MQ=255)                            gCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTTGCGTTTGCCgg                                                                                                           >  1:815276/1‑140 (MQ=255)                            gCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCgg                                                                                                           >  2:125149/1‑140 (MQ=255)                                      gTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGc                                                                                                 >  2:543826/1‑140 (MQ=255)                                             aaaGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGc                                                                                          <  1:543826/140‑1 (MQ=255)                                               aGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                              <  2:783205/134‑1 (MQ=255)                                               aGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                              >  1:783205/1‑134 (MQ=255)                                               aGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTg                                                                                        >  1:79919/1‑140 (MQ=255)                                               aGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGGGTCGGCCGCCCTTTCGGGGGATTGGCGTGTGCCCGAAAATTCCGCCGACCGCTg                                                                                        >  2:321808/1‑140 (MQ=255)                                                 cTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGcc                                                                                      >  2:555530/1‑140 (MQ=255)                                                  tACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCg                                                                                     <  1:534946/140‑1 (MQ=255)                                                        ggTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAAt                                                                               <  2:79919/140‑1 (MQ=255)                                                         gTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                              <  1:216202/124‑1 (MQ=255)                                                         gTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGa                                                                                              >  2:216202/1‑124 (MQ=255)                                                           cacaTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCCTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCa                                                                            >  2:534108/1‑140 (MQ=255)                                                              aTTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAAtt                                                                         >  1:351953/1‑140 (MQ=255)                                                                      gCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCaaa                                                                 <  1:820810/140‑1 (MQ=255)                                                                          tCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACggg                                                             <  2:58500/140‑1 (MQ=255)                                                                          tCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACggg                                                             <  1:766850/140‑1 (MQ=255)                                                                                  aTACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGAt                                                     <  1:222682/140‑1 (MQ=255)                                                                                               aGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTg                                        <  1:635085/140‑1 (MQ=255)                                                                                               aGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGGATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTg                                        >  2:392043/1‑140 (MQ=255)                                                                                                    aGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCt                                   >  2:97244/1‑140 (MQ=255)                                                                                                            gCTGACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGcaca                           <  1:206262/140‑1 (MQ=255)                                                                                                               gACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGc                        <  1:97244/140‑1 (MQ=255)                                                                                                               gACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGc                        >  1:922390/1‑140 (MQ=255)                                                                                                               gACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGc                        <  1:125149/140‑1 (MQ=255)                                                                                                               gACACGCGCCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGCATTGGCGCTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAAATGCGCCAAACGGGCAGGCGATTCCGCACAGCTTGCCGCTTACCCCCAGGc                        >  2:766371/1‑140 (MQ=255)                                                                                                                    gcgcCGGAGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttca                   <  2:922390/140‑1 (MQ=255)                                                                                                                           aGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcatt                 <  2:351612/135‑1 (MQ=255)                                                                                                                           aGCAGGCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTttcatt                 >  1:351612/1‑135 (MQ=255)                                                                                                                                gCGGTGTCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACg       >  2:743811/1‑140 (MQ=255)                                                                                                                                gCGGTGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCGCAGGCTTTCATTCAACTGCACg       >  1:695433/1‑140 (MQ=255)                                                                                                                                    tGCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGccc   >  1:584209/1‑140 (MQ=255)                                                                                                                                     gCCGGCGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGcccc  >  1:913020/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGCCGGTGGCGCTTTCGGGGTATTGGCGTTTGCCGGAATATCCAGCGGACGGCTGCCGGAAAATTCAATTGCGCCAAACGGGCAGGCGATGCCGCACAGCTTGCAGCTTACGCACAGGCTTTCATTCAACTGCACGGCCCC  >  NC_000913/2849362‑2849633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑