Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,571	(A)8→7	coding (701/1773 nt)	mdlA →	ABC transporter family protein MdlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,564	0	A	.	100.0%	127.0 / NA	31	coding (694/1773 nt)	mdlA	ABC transporter family protein MdlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/14);  total (17/14)

GCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGC  >  NC_000913/469431‑469695                                                                                                                                      |                                                                                                                                    gCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaaaaaaa                                                                                                                               <  2:315059/140‑1 (MQ=255)     gCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCaaaaaaacatg                                                                                                                           <  2:461312/140‑5 (MQ=255)           gcggcgTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTg                                                                                                                    <  2:208305/140‑1 (MQ=255)                        gTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCGTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTTCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATg                                                                                                       <  2:37936/140‑1 (MQ=255)                             aaTGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGt                                                                                                  >  2:165788/1‑140 (MQ=255)                              aTGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGtt                                                                                                 >  2:297313/1‑140 (MQ=255)                                gACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTc                                                                                               >  2:482138/1‑140 (MQ=255)                                   cGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGc                                                                                                      >  2:35483/1‑130 (MQ=255)                                   cGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGc                                                                                                      <  1:35483/130‑1 (MQ=255)                                   cGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgac                                                                                            <  1:297313/140‑1 (MQ=255)                                    gCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgacc                                                                                           <  1:334046/140‑1 (MQ=255)                                    gCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgacc                                                                                           <  2:328502/140‑1 (MQ=255)                                     cACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgaccc                                                                                          >  2:91767/1‑140 (MQ=255)                                      aCCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTcgacccg                                                                                         <  1:95451/140‑1 (MQ=255)                                                 ccTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTatat                                                                              >  1:392435/1‑140 (MQ=255)                                                    cACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATcgc                                                                           >  1:465288/1‑140 (MQ=255)                                                       cAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGAt                                                                        <  2:465288/140‑1 (MQ=255)                                                                 aTGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTGGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCg                                                              >  2:74818/1‑140 (MQ=255)                                                                  tGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGa                                                             >  1:14728/1‑140 (MQ=255)                                                                  tGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGa                                                             >  1:150708/1‑140 (MQ=255)                                                                    aTCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGA‑TGGTATGGCGAACt                                                          >  1:1198/1‑140 (MQ=255)                                                                              ttGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGa                                                 >  1:67453/1‑140 (MQ=255)                                                                               tGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGAt                                                >  1:33017/1‑140 (MQ=255)                                                                               tGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGAt                                                >  2:223534/1‑140 (MQ=255)                                                                                     ggAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTggcgg                                          <  2:392435/140‑1 (MQ=255)                                                                                         gATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGt                                      <  1:91767/140‑1 (MQ=255)                                                                                            cGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGt                                   >  2:214309/1‑140 (MQ=255)                                                                                                        ttATTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGgtggtg                       <  1:223534/140‑1 (MQ=255)                                                                                                          aTTTGCCGCGGATGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCa                     <  2:67453/140‑1 (MQ=255)                                                                                                                     aTGCCGAAGATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTaa          >  1:77330/1‑140 (MQ=255)                                                                                                                             gATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGc  >  2:515433/1‑140 (MQ=255)                                                                                                                             gATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGc  <  2:150708/140‑1 (MQ=255)                                                                                                                             gATACCGGC‑AAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGc  <  2:14728/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    GCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCAAAAAAAACATGCGGGTGGCGCGTATTGATGCTCGTTTCGACCCGACCATCTATATCGCGATTGGTATGGCGAACTTGCTGGCGATTGGCGGCGGTAGTTGGATGGTGGTGCAGGGCAGTTTAACGCTGGGC  >  NC_000913/469431‑469695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑