Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	881,516	(G)5→4	coding (790/1203 nt)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	881,512	0	G	.	100.0%	71.3 / NA	18	coding (786/1203 nt)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/9);  total (9/9)

GTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTG  >  NC_000913/881373‑881642                                                                                                                                            |                                                                                                                                   gTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTg                                                                                                                                    >  1:580081/1‑140 (MQ=255)            cgtaaccgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAacc                                                                                                                        <  2:540168/140‑1 (MQ=255)                accgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAaccgacc                                                                                                                    >  1:156274/1‑140 (MQ=255)                     ctgctgTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGATTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTCGTGTT‑GGGGCGAAAACCGACCGTATc                                                                                                               >  2:347875/1‑140 (MQ=255)                               gcagcaATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATg                                                                                                     <  2:251198/140‑1 (MQ=255)                                   caATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTc                                                                                                 <  2:237497/140‑1 (MQ=255)                                        tGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTgaga                                                                                            >  2:284300/1‑140 (MQ=255)                                                 aTGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGa                                                                                   >  1:74847/1‑140 (MQ=255)                                                               ggAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGc                                                                     <  2:555166/140‑1 (MQ=255)                                                                   ccACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGcttct                                                                 <  2:580081/140‑1 (MQ=255)                                                                             cgcgGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGt                                                       <  1:547654/140‑1 (MQ=255)                                                                                 ggATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACg                                                   <  2:156274/140‑1 (MQ=255)                                                                                            ggTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAAcc                                        >  1:225197/1‑140 (MQ=255)                                                                                            ggTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAAcc                                        >  1:397724/1‑140 (MQ=255)                                                                                               tGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGa                                     <  2:74847/140‑1 (MQ=255)                                                                                                                    tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:281568/1‑140 (MQ=255)                                                                                                                    tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:538360/1‑140 (MQ=255)                                                                                                                    tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:90488/1‑140 (MQ=255)                                                                                                                       gCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGt             <  2:225197/140‑1 (MQ=255)                                                                                                                                  ccGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTg  >  2:235634/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                   GTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTG  >  NC_000913/881373‑881642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑