Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,898	(G)6→8	coding (755/909 nt)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,892	1	.	G	100.0%	66.3 / NA	19	L250R (CTG→CGG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/11);  total (8/11)
*	NC_000913	410,892	2	.	G	100.0%	66.6 / NA	19	L250R (CTG→CGG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (8/11);  total (8/11)

GCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑GGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTAC  >  NC_000913/410755‑411020                                                                                                                                           ||                                                                                                                                 gcTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑gg                                                                                                                                <  1:100/140‑1 (MQ=255)      aaGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggg                                                                                                                           <  2:555635/140‑2 (MQ=255)        ggCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggggc                                                                                                                         >  1:220354/1‑137 (MQ=255)        ggCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggggc                                                                                                                         <  2:596984/140‑4 (MQ=255)          cAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggggcgg                                                                                                                       >  2:376090/1‑135 (MQ=255)               aaaTTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGa                                                                                                                    >  1:71058/1‑140 (MQ=255)                 aTTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGc                                                                                                                  <  2:553347/140‑1 (MQ=255)                      ccGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGt                                                                                                             <  1:376090/140‑1 (MQ=255)                        gCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAg                                                                                                           <  2:220354/140‑1 (MQ=255)                        gCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAg                                                                                                           <  2:354690/140‑1 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATg                                                                                                              >  1:565819/1‑136 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATg                                                                                                              <  2:565819/136‑1 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGa                                                                                                          <  2:64512/140‑1 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGa                                                                                                          <  1:210783/140‑1 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggggc                                                                                                                         >  1:53201/1‑120 (MQ=255)                         ccTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑ggggggggc                                                                                                                         <  2:53201/123‑4 (MQ=255)                                       gCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCa                                                                                              <  1:31643/138‑1 (MQ=255)                                       gCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCa                                                                                              >  2:31643/1‑138 (MQ=255)                                          aTCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACgg                                                                                         <  2:71058/140‑1 (MQ=255)                                           tCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACgg                                                                                         >  2:194421/1‑139 (MQ=255)                                           tCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACgg                                                                                         <  1:194421/139‑1 (MQ=255)                                                              ttGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACaa                                                                     >  2:448480/1‑140 (MQ=255)                                                                           aGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCGATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTcggcg                                                        >  2:410684/1‑140 (MQ=255)                                                                                                            tGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGc                       >  1:235147/1‑140 (MQ=255)                                                                                                              aaTATTCTCGATCCGGATGTGATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCAc                     <  2:235147/140‑1 (MQ=255)                                                                                                                                 tgATTGTCCTGGGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTAc  >  2:31797/1‑140 (MQ=255)                                                                                                                                           ||                                                                                                                                 GCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCT‑‑GGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTAC  >  NC_000913/410755‑411020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑