Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,812	(C)6→7	coding (204/459 nt)	mgsA ←	methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,806	1	.	C	100.0%	113.8 / NA	32	G70G (GGT→GGG)	mgsA	methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (18/14);  total (18/14)

ACCGGAATGTTCCATACCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCA‑CC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATC  >  NC_000913/1026669‑1026938                                                                                                                                           |                                                                                                                                      aCCGGAATGTTCCATACCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCA‑cc                                                                                                                                     >  2:429666/1‑140 (MQ=255)       aTGTTCCATACCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCA‑cc‑ccccca                                                                                                                              <  1:429666/140‑3 (MQ=255)           tCCATACCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTgg                                                                                                                           >  1:127263/1‑140 (MQ=255)                aCCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCAc                                                                                                                      >  2:179890/1‑140 (MQ=255)                aCCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCAc                                                                                                                      <  1:576316/140‑1 (MQ=255)                 ccGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACt                                                                                                                     <  1:341059/140‑1 (MQ=255)                    tCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCaa                                                                                                                  >  2:207715/1‑140 (MQ=255)                                 caAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACg                                                                                                     <  1:309813/140‑1 (MQ=255)                                        ttCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGc                                                                                              >  1:259977/1‑140 (MQ=255)                                         tCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGcc                                                                                             >  2:366677/1‑140 (MQ=255)                                          cACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCA‑CCACCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCg                                                                                            >  2:1313/1‑140 (MQ=255)                                                       tGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGaaa                                                                               >  1:64083/1‑140 (MQ=255)                                                              cggcaTTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGtt                                                                        >  1:168151/1‑140 (MQ=255)                                                              cggcaTTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGtt                                                                        <  1:366677/140‑1 (MQ=255)                                                                       gTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGt                                                               <  2:64083/140‑1 (MQ=255)                                                                       gTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGt                                                               <  1:327619/140‑1 (MQ=255)                                                                         ggATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGc                                                             <  1:207715/140‑1 (MQ=255)                                                                           aTCCCAGAAGAAAATCAATCCATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCt                                                           >  1:332342/1‑140 (MQ=255)                                                                           aTCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCt                                                           >  1:585904/1‑140 (MQ=255)                                                                                 gaagaaAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTg                                                          >  1:450418/1‑135 (MQ=255)                                                                                 gaagaaAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTg                                                          <  2:450418/135‑1 (MQ=255)                                                                                     aaaaTCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACa                                                 <  1:324990/140‑1 (MQ=255)                                                                                          cAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACg                                            <  2:585904/140‑1 (MQ=255)                                                                                                   aaTTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTcc                                   >  1:158642/1‑140 (MQ=255)                                                                                                     ttttCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAg                                 >  2:129107/1‑140 (MQ=255)                                                                                                          ccTTCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACg                            >  1:53297/1‑140 (MQ=255)                                                                                                            ttCTGAGATCAATGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGt                          <  2:332342/140‑1 (MQ=255)                                                                                                                        tGCGCCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTcc              >  2:206073/1‑140 (MQ=255)                                                                                                                           gcCAACCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCAcc           >  1:188992/1‑140 (MQ=255)                                                                                                                              aaCCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAg        >  2:108751/1‑140 (MQ=255)                                                                                                                               aCCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                     <  2:533480/126‑1 (MQ=255)                                                                                                                               aCCTGCTGGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATg                     >  1:533480/1‑126 (MQ=255)                                                                                                                                    ctgGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTGCGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCtcatc  <  2:53297/140‑1 (MQ=255)                                                                                                                                    ctgGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCtcat   >  2:389465/1‑139 (MQ=255)                                                                                                                                    ctgGTCACCC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCtcat   <  1:389465/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      ACCGGAATGTTCCATACCGTCGCCAGACGCAGCAAGGCTTTCACGTCAGGATCGTGCGGCACGGCATTTAGTGGATCCCAGAAGAAAATCAATACATCAATTTTCCCTTCTGAGATCAATGCGCCAACCTGCTGGTCA‑CC‑CCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATC  >  NC_000913/1026669‑1026938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑