| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,894,349 | (A)8→9 | coding (217/300 nt) | yoaC → | DUF1889 domain‑containing protein YoaC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,894,341 | 1 | . | A | 95.3% | 144.5 / ‑0.4 | 43 | coding (209/300 nt) | yoaC | DUF1889 domain‑containing protein YoaC |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (15/26); total (16/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
TGAAAGCACGGCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑AAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAAT > NC_000913/1894210‑1894474 | tgaaAGCACGGCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑aaaaaaaa > 1:178258/1‑140 (MQ=255) aaaGCACGGCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑aaaaaaaaat < 2:331135/140‑3 (MQ=255) aCGGCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGgaga < 1:347489/140‑1 (MQ=255) ggCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACa < 1:204197/140‑1 (MQ=255) cGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAgg < 1:95322/140‑1 (MQ=255) aaGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTg > 1:593892/1‑140 (MQ=255) aTCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGtt < 2:184798/140‑1 (MQ=255) aaTATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGAt > 2:524087/1‑140 (MQ=255) ttAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTaaaa > 1:256641/1‑140 (MQ=255) tGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATa < 2:74802/140‑1 (MQ=255) tGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATa < 1:21645/140‑1 (MQ=255) ggAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTaaaa > 1:185594/1‑128 (MQ=255) ggAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTaaaa < 2:185594/128‑1 (MQ=255) aGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACttt < 2:522288/140‑1 (MQ=255) gTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACtttt < 1:379818/140‑1 (MQ=255) tACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTc < 1:460875/140‑1 (MQ=255) tACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTc < 2:178258/140‑1 (MQ=255) aCCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCa > 2:445686/1‑140 (MQ=255) cgcgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACa < 2:146762/140‑1 (MQ=255) cgcgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACa < 1:531770/140‑1 (MQ=255) cgcgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑AAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACa < 2:205029/139‑1 (MQ=255) cgcgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑AAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACa > 1:205029/1‑139 (MQ=255) cgAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACata > 1:544689/1‑140 (MQ=255) tGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATg > 2:570057/1‑140 (MQ=255) ggCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTaaaa > 2:593647/1‑140 (MQ=255) gcgAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCa > 2:432757/1‑140 (MQ=255) cgAGCTGACCAGGAAGGTTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCAc > 1:467802/1‑140 (MQ=255) gAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACt > 1:271263/1‑140 (MQ=255) gCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTgg < 2:256641/140‑1 (MQ=255) cTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGt < 2:593892/140‑1 (MQ=255) gACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCt < 1:570057/140‑1 (MQ=255) ggAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGtt < 2:555550/140‑1 (MQ=255) gAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAGCTAAAAGCACTGGTCTGAGTTa > 1:436163/1‑140 (MQ=255) aGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTaaa < 1:503992/140‑1 (MQ=255) cTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGtt < 1:366425/134‑1 (MQ=255) cTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGtt > 2:366425/1‑134 (MQ=255) ggAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTtat > 1:284015/1‑140 (MQ=255) aaCCCTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTatat < 2:467802/140‑1 (MQ=255) cTGGGTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGc < 1:432757/140‑1 (MQ=255) ggTTCACGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATa < 2:271263/140‑1 (MQ=255) cGGAAAAAATGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATggg > 1:508415/1‑140 (MQ=255) aaaTGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGa < 2:324117/140‑1 (MQ=255) aaaTGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGa < 2:284015/140‑1 (MQ=255) aaaTGGTTGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGa < 1:259825/140‑1 (MQ=255) tGGATGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCtttt > 2:235266/1‑140 (MQ=255) aTGGGCAAAAAAAAATGGAGTCAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAAt < 1:593647/140‑1 (MQ=255) aTGGGCAAAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAAt < 2:436163/140‑1 (MQ=255) | TGAAAGCACGGCGAAGGGAATCTTTAAATATTTAAAAGAACTGGGAGTACCCGCGAGTGCCGCTGATATTACGGCGCGAGCTGACCAGGAAGGCTGGAACCCTGGGTTCACGGAAAAAATGGTTGGATGGGC‑AAAAAAAATGGAGACAGGTGAACGTTCTGTGATTAAAAATCCTGAATACTTTTCAACATATATGCAGGAAGAACTAAAAGCACTGGTCTGAGTTAAATTTATATCAGCATAAATGGGTCAGGACGCTTTTAAT > NC_000913/1894210‑1894474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |