BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,120,438	(C)_6→7	coding (1117/1395 nt)	wcaJ ←	undecaprenyl‑phosphate glucose phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,432	1	.	C	100.0%	120.2 / NA	34	M375V (ATG→GTG)	wcaJ	undecaprenyl‑phosphate glucose phosphotransferase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (20/14); total (20/14)

GTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑CCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCA  >  NC_000913/2120293‑2120560

gTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAt                                                                                                                                   <  2:393198/140‑1 (MQ=255)

             aGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAg                                                                                                                      <  1:605360/140‑1 (MQ=255)

                         gcgcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                    <  2:98778/130‑1 (MQ=255)

                         gcgcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                    >  1:98778/1‑130 (MQ=255)

                              cAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATGCTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCg                                                                                                     <  2:26423/140‑1 (MQ=255)

                                        tGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATc                                                                                           <  2:392625/140‑1 (MQ=255)

                                           ccGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATc                                                                                           >  2:281259/1‑137 (MQ=255)

                                           ccGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATc                                                                                           <  1:281259/137‑1 (MQ=255)

                                            cGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGc                                                                                       >  1:100625/1‑140 (MQ=255)

                                              gTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGa                                                                                     >  2:331182/1‑140 (MQ=255)

                                               tttCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAg                                                                                    >  2:91082/1‑140 (MQ=255)

                                                   aCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTAc                                                                                >  1:179729/1‑140 (MQ=255)

                                                        aTGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGGTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCAGCCAGCGAGGTACGgcgc                                                                           >  2:548935/1‑140 (MQ=255)

                                                              cagcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACg                                                                               >  2:556236/1‑130 (MQ=255)

                                                              cagcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACg                                                                               <  1:556236/130‑1 (MQ=255)

                                                               agcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGt                                                                    >  1:55138/1‑140 (MQ=255)

                                                                   tGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTccc                                                                <  1:91082/140‑1 (MQ=255)

                                                                                      tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGa                                             >  1:325793/1‑140 (MQ=255)

                                                                                      tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGa                                             <  2:179729/140‑1 (MQ=255)

                                                                                      tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCAACTTTGGTGACGCGCGGa                                             <  1:244413/140‑1 (MQ=255)

                                                                                               ttCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGc                                    >  1:343489/1‑140 (MQ=255)

                                                                                                tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCg                                   <  1:33222/140‑1 (MQ=255)

                                                                                                tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCg                                   >  1:3091/1‑140 (MQ=255)

                                                                                                 cGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGt                                  >  1:547709/1‑140 (MQ=255)

                                                                                                            aCTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCg                                   >  2:103955/1‑128 (MQ=255)

                                                                                                            aCTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCg                                   <  1:103955/128‑1 (MQ=255)

                                                                                                             cTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTcac                      >  2:296861/1‑140 (MQ=255)

                                                                                                             cTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTcac                      >  2:393438/1‑140 (MQ=255)

                                                                                                              tgcgtgcgGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTcacc                     >  2:114295/1‑140 (MQ=255)

                                                                                                                cgtgcgGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTcaccac                   >  2:47295/1‑140 (MQ=255)

                                                                                                                     ggACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGt              <  2:343489/140‑1 (MQ=255)

                                                                                                                       aCGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCg            <  1:548935/140‑1 (MQ=255)

                                                                                                                              ccGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCa     >  2:291823/1‑140 (MQ=255)

                                                                                                                                 aCAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCa  <  2:193445/140‑1 (MQ=255)

                                                                                                                                 aCAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCa  >  1:193445/1‑140 (MQ=255)

GTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑CCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCA  >  NC_000913/2120293‑2120560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`