Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	93,282	A→G	V39V (GTA→GTG)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	93,282	0	A	G	100.0%	81.2 / NA	25	V39V (GTA→GTG)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/17);  total (8/17)

TAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCG  >  NC_000913/93146‑93414                                                                                                                                         |                                                                                                                                     tAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCt                                                                                                                                   >  1:298825/1‑140 (MQ=255)  aaTCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTg                                                                                                                                  <  2:347364/140‑1 (MQ=255)        ggCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGtagtag                                                                                                                            >  2:230718/1‑140 (MQ=255)            aGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGtca                                                                                                                        <  2:121489/140‑1 (MQ=255)             ggggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGGTCTCTTTGTGGCTGTAGTAGGgcat                                                                                                                       >  1:234099/1‑140 (MQ=255)             ggggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGtcat                                                                                                                       >  2:413904/1‑140 (MQ=255)              gggACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGgcatc                                                                                                                      >  1:62682/1‑140 (MQ=255)                  cAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGc                                                                                                                  >  1:553775/1‑140 (MQ=255)                       ggCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGATAGATGGCACTCGACAGTCGTGTTGCTGCGGCGGGTGATCTCGTTGTGGCTGTAGTAGGTCATCAGGCGGACg                                                                                                             <  2:40634/140‑1 (MQ=255)                         cAGATCGTAATTTGCGCGGCCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACggg                                                                                                           >  2:543684/1‑140 (MQ=255)                          aGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGGAGGAGGGCATCAGGcgggcgggg                                                                                                          >  2:106229/1‑139 (MQ=255)                           gATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                                                         <  2:62682/140‑1 (MQ=255)                           gATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                                                         <  1:548632/140‑1 (MQ=255)                             tCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                                                         <  2:568545/138‑1 (MQ=255)                             tCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCg                                                                                                         >  1:568545/1‑138 (MQ=255)                                       cgcgACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGGTCTCTTTGTGGCTGTAGGAGGGCATCCGGCGGGCCGGCGGCGATATATccc                                                                                             >  2:12406/1‑140 (MQ=255)                                       cgcgACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGGCATCAGGCGGGCGGGCGGCGATATATccc                                                                                             >  2:145405/1‑140 (MQ=255)                                          gACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCa                                                                                          <  2:360994/140‑1 (MQ=255)                                          gACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCa                                                                                          <  2:553775/140‑1 (MQ=255)                                              ttcttGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCg                                                                                      <  1:106229/140‑1 (MQ=255)                                                         tGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAgg                                                                           >  2:281120/1‑140 (MQ=255)                                                           ggTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGgtg                                                                         <  1:281120/140‑1 (MQ=255)                                                           ggTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGgtg                                                                         <  1:230718/140‑1 (MQ=255)                                                                cAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCt                                                                    <  1:413904/140‑1 (MQ=255)                                                                            cgcgAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCa                                                        <  2:576879/140‑1 (MQ=255)                                                                                                       aCAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGt                             <  2:298825/140‑1 (MQ=255)                                                                                                       aCAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGt                             <  1:543684/140‑1 (MQ=255)                                                                                                           ccGTGTGGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGaaa                         >  1:467746/1‑140 (MQ=255)                                                                                                              tgtgGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATcc                      <  1:145405/140‑1 (MQ=255)                                                                                                              tgtgGCTGCGGCGGGCGATCTCTTTGTGGCTGTAGTAGGGCATCAGGCGGGCGGGGGTCGATATATCCCGCAGGCGATAGCGCCAGGGGTGGCTGCCATTATTTCAGAGGCGAAAGATGAGGCGACCGATGGTGGAAAcc                      >  2:401621/1‑140 (MQ=255)                                                                                                                        gcgggcgATCTCTTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCa            <  2:467746/140‑1 (MQ=255)                                                                                                                                  tctTTGTGGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCg  <  2:234099/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TAATCAAGGCGAGGGGACAGGTGGCAGATCGTAATTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGGCTGCGGCGGGCGATCTCTTTGTAGCTGTAGTAGGTCATCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCG  >  NC_000913/93146‑93414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑