Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	416,658	A→G	F99L (TTC→CTC)	sbcD ←	ATP dependent, structure specific DNA nuclease ‑ SbcD subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	416,658	0	A	G	100.0%	89.6 / NA	26	F99L (TTC→CTC)	sbcD	ATP dependent, structure specific DNA nuclease ‑ SbcD subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CCCCGCCTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGA  >  NC_000913/416524‑416793                                                                                                                                       |                                                                                                                                        ccccGCCTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCa                                                                                                                                    >  2:3570/1‑140 (MQ=255) ccccGCCTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCa                                                                                                                                    >  2:409921/1‑140 (MQ=255)     gCCTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGat                                                                                                                                <  1:3570/140‑1 (MQ=255)      ccTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGata                                                                                                                               >  2:275875/1‑140 (MQ=255)      ccTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGata                                                                                                                               >  1:373449/1‑140 (MQ=255)                  taatGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGattca                                                                                                                   <  2:598759/140‑1 (MQ=255)                        cACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGc                                                                                                             >  1:227976/1‑140 (MQ=255)                          cgcgGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGt                                                                                                           >  2:405717/1‑140 (MQ=255)                            cgGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTgg                                                                                                         >  2:261438/1‑140 (MQ=255)                                        cGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCAt                                                                                             <  2:373449/140‑1 (MQ=255)                                             tGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGttt                                                                                        <  1:405717/140‑1 (MQ=255)                                              ggggCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTc                                                                                       <  1:261438/140‑1 (MQ=255)                                                     aGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAg                                                                                <  1:186410/140‑1 (MQ=255)                                                        aCTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTac                                                                             <  1:409921/140‑1 (MQ=255)                                                          tGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTacca                                                                           >  2:51517/1‑140 (MQ=255)                                                                 ggCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATg                                                                    >  1:349748/1‑140 (MQ=255)                                                                               acgaGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCt                                                      <  2:349748/140‑1 (MQ=255)                                                                                 gaGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGt                                                    <  2:227976/140‑1 (MQ=255)                                                                                             gcggcgCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGacaaca                                        >  2:162493/1‑140 (MQ=255)                                                                                                gcgcATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                     <  2:250052/140‑1 (MQ=255)                                                                                                  gcATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACg                                   <  1:275875/140‑1 (MQ=255)                                                                                                             gcTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                     >  1:69304/1‑127 (MQ=255)                                                                                                             gcTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACaaaa                                     <  2:69304/127‑1 (MQ=255)                                                                                                              cTGGCGACCACGGTAGTATTGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGt                       <  1:355121/140‑1 (MQ=255)                                                                                                                                ttGAGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGggcgg     <  1:162493/140‑1 (MQ=255)                                                                                                                                   aGGAGCGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGa  >  1:615517/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CCCCGCCTGGCTGGTAATAATGTCACGCGGACGTAAAAACGGAATGGGGCACAGCACTGCGCCTGGCGTCCCGTCGCGACGAGGCAAGATTTGCGGCGCATGTCCGGCGCTGGCGACCACGGTAGTATTGAGGAACGCCATGATATCGCGCGATTCATTCAGCGTGGCGACCGAGTCATGGTTTCCTGCCAGTACCACCAGATGACAGCCAGTTTGCTGTAAATTGACAACAAAACGGTTGTATAACGTGCGGGCGTAACTGGGCGGCGA  >  NC_000913/416524‑416793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑