Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	517,173	A→G	L197L (TTA→TTG)	fetB →	putative iron ABC exporter membrane subunit FetB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	517,173	0	A	G	100.0%	159.2 / NA	44	L197L (TTA→TTG)	fetB	putative iron ABC exporter membrane subunit FetB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/26);  total (18/26)

AACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTA  >  NC_000913/517037‑517309                                                                                                                                         |                                                                                                                                         aaCAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCa                                                                                                                                       >  2:197181/1‑140 (MQ=255) aaCAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCa                                                                                                                                       >  1:482095/1‑140 (MQ=255)            aTTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTc                                                                                                                            <  2:221138/140‑1 (MQ=255)                aGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCggg                                                                                                                        <  1:252246/140‑1 (MQ=255)                       aCTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGata                                                                                                                  <  2:255386/139‑1 (MQ=255)                       aCTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGata                                                                                                                  >  1:255386/1‑139 (MQ=255)                           aGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGc                                                                                                             <  1:512334/140‑1 (MQ=255)                           aGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGc                                                                                                             <  1:499746/140‑1 (MQ=255)                            gTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGcc                                                                                                            >  1:111713/1‑140 (MQ=255)                                  gTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGAtt                                                                                                      >  2:420015/1‑140 (MQ=255)                                    gCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGa                                                                                                    <  2:277977/140‑1 (MQ=255)                                       aCGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGAt                                                                                                   <  2:458237/138‑1 (MQ=255)                                       aCGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGAt                                                                                                   >  1:458237/1‑138 (MQ=255)                                       aCGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATcc                                                                                                 >  1:312055/1‑140 (MQ=255)                                                    cTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGAt                                                                                                   <  2:34541/125‑1 (MQ=255)                                                    cTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGAt                                                                                                   >  1:34541/1‑125 (MQ=255)                                                    cTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGAtt                                                                                    <  1:522635/140‑1 (MQ=255)                                                     ttCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTa                                                                                   <  1:345018/140‑1 (MQ=255)                                                         gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAata                                                                               <  2:304097/140‑1 (MQ=255)                                                         gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAata                                                                               <  2:12037/140‑1 (MQ=255)                                                         gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAata                                                                               <  1:168960/140‑1 (MQ=255)                                                         gCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAata                                                                               <  2:482095/140‑1 (MQ=255)                                                          cGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAatat                                                                              >  2:551866/1‑140 (MQ=255)                                                               ttGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGAt                                                                         >  1:219989/1‑140 (MQ=255)                                                                    tCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGAt                                                                         >  1:611027/1‑135 (MQ=255)                                                                    tCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGAt                                                                         <  2:611027/135‑1 (MQ=255)                                                                    tCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATgg                                                                    >  2:146140/1‑140 (MQ=255)                                                                     cgcgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGt                                                                   <  1:420015/140‑1 (MQ=255)                                                                       cgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGa                                                                 >  1:401607/1‑140 (MQ=255)                                                                       cgACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGa                                                                 >  1:79229/1‑140 (MQ=255)                                                                                  gcgcgGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTAtgctg                                                      >  2:177890/1‑140 (MQ=255)                                                                                            aaTTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCg                                            <  2:79229/140‑1 (MQ=255)                                                                                                  gACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCt                                      >  2:420224/1‑140 (MQ=255)                                                                                                  gACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTGATGCTGCTCTCAACCGCCAGCt                                      >  1:436196/1‑140 (MQ=255)                                                                                                      gTCGATTCAGCAAAAGCGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCCGCTCTCACCCGCCAGCTTGTc                                  <  2:376118/140‑1 (MQ=255)                                                                                                       tCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCg                                 <  2:111713/140‑1 (MQ=255)                                                                                                        cGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGa                                <  2:312055/140‑1 (MQ=255)                                                                                                                   aaaCGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGcc                     <  1:197181/140‑1 (MQ=255)                                                                                                                   aaaCGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGcc                     <  1:177890/140‑1 (MQ=255)                                                                                                                     aCGGTTGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTg                   <  1:420224/140‑1 (MQ=255)                                                                                                                         ttGGCTTAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTAt               <  1:146140/140‑1 (MQ=255)                                                                                                                              ttAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAAc          <  2:219989/140‑1 (MQ=255)                                                                                                                              ttAGTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAAc          <  2:401607/140‑1 (MQ=255)                                                                                                                                 gTGAGTTTGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTa       <  2:436196/140‑1 (MQ=255)                                                                                                                                      tttGCCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTa  >  1:36747/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         AACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTATTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATGTCCGGGCTGATATTTGCCGGGATTGATCCGGTGAAGGCGATTAAATATCAGATTATGGTGACCTTTATGCTGCTCTCAACCGCCAGCTTGTCGACCATTATTGCCTGCTATTTAACCTATCGTA  >  NC_000913/517037‑517309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑