Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	877,873	A→G	F55L (TTT→CTT)	rimO ←	ribosomal protein S12 methylthiotransferase RimO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	877,873	0	A	G	100.0%	146.2 / NA	40	F55L (TTT→CTT)	rimO	ribosomal protein S12 methylthiotransferase RimO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

TTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGATT  >  NC_000913/877738‑878009                                                                                                                                        |                                                                                                                                         tttCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCg                                                                                                                                      <  2:330266/140‑1 (MQ=255) tttCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCg                                                                                                                                      <  1:317419/140‑1 (MQ=255)             tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  2:173039/1‑140 (MQ=255)             tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  1:486102/1‑140 (MQ=255)             tCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACg                                                                                                                          >  1:442100/1‑140 (MQ=255)                            ggATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCa                                                                                                           <  2:459687/140‑1 (MQ=255)                            ggATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCa                                                                                                           <  1:349328/140‑1 (MQ=255)                                  gatctTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCa                                                                                                     <  1:260394/140‑1 (MQ=255)                                       tcttTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCt                                                                                                <  2:126204/140‑1 (MQ=255)                                          tttGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCgg                                                                                             >  1:167305/1‑140 (MQ=255)                                           ttGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGt                                                                                            >  2:511684/1‑140 (MQ=255)                                              cccccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTAcc                                                                                         <  1:376637/140‑1 (MQ=255)                                              cccccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTAcc                                                                                         >  1:175569/1‑140 (MQ=255)                                                cccAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCAc                                                                                       >  1:265893/1‑140 (MQ=255)                                                         ccGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAAcc                                                                              >  2:315977/1‑140 (MQ=255)                                                         ccGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAAcc                                                                              >  1:585564/1‑140 (MQ=255)                                                          cGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCt                                                                             <  2:265893/140‑1 (MQ=255)                                                             tCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTATCACGTCATAACCTTCa                                                                          >  1:255155/1‑140 (MQ=255)                                                                       cTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGtt                                                                <  1:315977/140‑1 (MQ=255)                                                                          gCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCgg                                                             <  1:173039/140‑1 (MQ=255)                                                                                  cattcaACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATa                                                     <  1:511684/140‑1 (MQ=255)                                                                                      caACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGct                                                 <  2:442100/140‑1 (MQ=255)                                                                                                   aTGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAg                                    >  2:94654/1‑140 (MQ=255)                                                                                                   aTGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAg                                    >  2:62102/1‑140 (MQ=255)                                                                                                        ttCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAACTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               >  2:472970/1‑140 (MQ=255)                                                                                                         tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               <  1:332054/139‑1 (MQ=255)                                                                                                         tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtttt                               >  2:332054/1‑139 (MQ=255)                                                                                                         tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtt                                 <  2:591949/137‑1 (MQ=255)                                                                                                         tCCAGTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGtt                                 >  1:591949/1‑137 (MQ=255)                                                                                                             gTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          <  2:486102/140‑1 (MQ=255)                                                                                                             gTGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAACACGCTCTGAATCAACAAGGTTTTTCGGa                          >  2:35544/1‑140 (MQ=255)                                                                                                              tGATTCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGAc                         >  1:425640/1‑140 (MQ=255)                                                                                                                 ttCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGCGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGc                      <  2:585564/140‑1 (MQ=255)                                                                                                                  tCTTGTACCGCGCTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGcc                     <  2:167305/140‑1 (MQ=255)                                                                                                                            cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          >  1:65908/1‑125 (MQ=255)                                                                                                                            cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGa                          <  2:65908/125‑1 (MQ=255)                                                                                                                            cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACa           >  2:113374/1‑140 (MQ=255)                                                                                                                            cgcTGTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACa           >  1:465067/1‑140 (MQ=255)                                                                                                                                gTCAATAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGc       <  2:66200/140‑1 (MQ=255)                                                                                                                                     tAAGGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTATCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTAAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGAtt  <  2:255155/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGCTTCACCAATGGCTTCCAGTGATTCTTGTACCGCGCTGTCAATAAAGCCGCAGGTGTTGACGATCACCATGTCCGCATCGTCATAGCTCGGTACCACGTCATAACCTTCAGTGCGGAGTTCGGTGAGAATACGCTCTGAATCAACAAGGTTTTTCGGACAGCCAAGGGAAACAAAGCCGATT  >  NC_000913/877738‑878009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑