Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,002,435	A→G	Y210C (TAC→TGC)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,002,435	0	A	G	100.0%	99.2 / NA	28	Y210C (TAC→TGC)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

AAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG  >  NC_000913/1002308‑1002565                                                                                                                                |                                                                                                                                   aaaaTATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTAc                                                                                                                        <  2:565713/140‑1 (MQ=255)    atatTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGAt                                                                                                                     <  1:54927/140‑1 (MQ=255)       ttGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCaa                                                                                                                  <  2:324123/140‑1 (MQ=255)                ttcgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTgg                                                                                                         >  2:399157/1‑140 (MQ=255)                  cgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCt                                                                                                       <  2:543196/140‑1 (MQ=255)                  cgttGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGCACACCGGTTTTCGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCt                                                                                                       >  1:474646/1‑140 (MQ=255)                        cTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGtt                                                                                                 <  1:170202/140‑1 (MQ=255)                                     cctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCgg                                                                                    <  1:51559/140‑1 (MQ=255)                                     cctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCgg                                                                                    <  1:188613/140‑1 (MQ=255)                                      ctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGga                                                                                   <  1:399157/140‑1 (MQ=255)                                      ctcctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGga                                                                                   <  2:304070/140‑1 (MQ=255)                                         ctATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGgagag                                                                                <  1:544826/140‑1 (MQ=255)                                           aTGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGAc                                                                              >  1:295511/1‑140 (MQ=255)                                                gctggcgcGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTg                                                                         >  2:418328/1‑140 (MQ=255)                                                               tGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTg                                                          >  1:380021/1‑140 (MQ=255)                                                                    gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGt                                                                     <  1:435372/124‑1 (MQ=255)                                                                    gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGt                                                                     >  2:435372/1‑124 (MQ=255)                                                                    gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTc                                                     >  1:30902/1‑140 (MQ=255)                                                                    gCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTc                                                     >  2:124724/1‑140 (MQ=255)                                                                        caacaTACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCg                                                 >  1:557103/1‑140 (MQ=255)                                                                              aCCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCa                                           >  1:74483/1‑140 (MQ=255)                                                                                            ccTGCACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTa                             <  2:295511/140‑1 (MQ=255)                                                                                                cACGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGc                         >  2:238137/1‑140 (MQ=255)                                                                                                 aCGACTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCa                        <  2:380021/140‑1 (MQ=255)                                                                                                    aCTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                     >  1:464530/1‑140 (MQ=255)                                                                                                    aCTACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATaa                     <  1:238137/140‑1 (MQ=255)                                                                                                      tACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                   <  2:464530/140‑1 (MQ=255)                                                                                                      tACAGGTACACCGGTTTTAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAAc                   <  2:474646/140‑1 (MQ=255)                                                                                                                       tAGTGTATTGCCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACg  <  2:74483/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                   AAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTATGGCGCTGGCGCGATTATATGAATGCTACAACATACCCGCAGGTGATTCCTGCACGACTACAGGTACACCGGTTTTAGTGTATTACCTGTCTGGTACGATCAATTCACTTGGCTCATGTTCCGTCAATGCCGGAGAGACAATTGAAGTTGATTTAGGTGATGTCTTCGCTGCCAATTTCCGTGTTGTAGGGCATAAACCTCTTGGGGCCAGAACG  >  NC_000913/1002308‑1002565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑