| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,243,195 | T→C | F6L (TTT→CTT) | emtA → | lytic murein transglycosylase E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,243,195 | 0 | T | C | 97.4% | 126.5 / ‑4.0 | 39 | F6L (TTT→CTT) | emtA | lytic murein transglycosylase E |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (19/19); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
GTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGT > NC_000913/1243066‑1243320 | gTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCttttt < 2:442813/140‑1 (MQ=255) aaCAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGAtt < 1:404464/140‑1 (MQ=255) agaCATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGtt > 1:533343/1‑140 (MQ=255) aCATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGttat > 1:179744/1‑140 (MQ=255) gCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCg > 2:51860/1‑140 (MQ=255) gCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCg > 2:247363/1‑140 (MQ=255) aaTTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCggg < 2:179744/140‑1 (MQ=255) aaTTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCggg < 1:51860/140‑1 (MQ=255) aTTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGgt > 1:109572/1‑140 (MQ=255) aTTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGgt > 1:455593/1‑140 (MQ=255) gCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTCCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTtcat < 2:570315/140‑1 (MQ=255) caacaGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAg < 2:331770/140‑1 (MQ=255) acaGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCa < 1:436634/140‑1 (MQ=255) acaGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCa < 2:533343/140‑1 (MQ=255) caGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCAt < 2:282777/140‑1 (MQ=255) gAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGa < 2:109572/140‑1 (MQ=255) aaaCGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGAc < 1:564393/140‑1 (MQ=255) gTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAAc < 1:247363/140‑1 (MQ=255) gTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTAATCAAAGCATGACTATACGAAc < 2:455593/140‑1 (MQ=255) tataTCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAAcc < 2:330065/140‑1 (MQ=255) cGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGaa > 1:491860/1‑140 (MQ=255) cGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGaa > 2:500133/1‑140 (MQ=255) cGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATCGAGATGGCTTGCCTTTTTGATTGTTTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGaa > 2:478558/1‑140 (MQ=255) cTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGt > 1:102521/1‑140 (MQ=255) aaCGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCg > 2:307301/1‑140 (MQ=255) cGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGAt < 2:583957/140‑1 (MQ=255) cGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATTGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCa > 2:216314/1‑140 (MQ=255) aTTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCa < 1:43567/138‑1 (MQ=255) aTTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCa > 2:43567/1‑138 (MQ=255) aTTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGt < 2:102521/140‑1 (MQ=255) ggCTATAAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGc > 1:528480/1‑140 (MQ=255) taAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATa > 1:596340/1‑140 (MQ=255) taAGGATAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATa > 2:318641/1‑140 (MQ=255) tAGAAGAAGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAg > 2:463906/1‑140 (MQ=255) gaagaaGTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGaa > 1:119604/1‑140 (MQ=255) agaagTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAg > 2:514763/1‑140 (MQ=255) gaagTGAAATTGAGATGGCTTGCTTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGc < 2:117672/140‑1 (MQ=255) gTGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCgg > 2:194492/1‑140 (MQ=255) tGAAATTGAGATGGCTTGCTTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGt < 1:500038/140‑1 (MQ=255) tGAAATTGAGATGGCTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGt < 1:307301/140‑1 (MQ=255) | GTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTATATCATGCCGCTTAGGTGTGCCGTTGTCACCTCAACGGCGATTCCAGGCTATAAGGATAGAAGAAGTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCATGACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATGCCAATAAGCCAGAAAGCCGGT > NC_000913/1243066‑1243320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |