Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,258,755	T→C	intergenic (‑242/‑36)	pth ← / → ychH	peptidyl‑tRNA hydrolase/stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,258,755	0	T	C	100.0%	149.5 / NA	41	intergenic (‑242/‑36)	pth/ychH	peptidyl‑tRNA hydrolase/stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/21);  total (20/21)

AGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAC  >  NC_000913/1258619‑1258890                                                                                                                                         |                                                                                                                                        aGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATcccc                                                                                                                                      >  2:214906/1‑140 (MQ=255) aGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATcccc                                                                                                                                      >  2:166087/1‑140 (MQ=255) aGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATcccc                                                                                                                                      <  1:453578/140‑1 (MQ=255)     gACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGtt                                                                                                                                  >  1:38324/1‑140 (MQ=255)      aCTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTa                                                                                                                                 <  2:145454/140‑1 (MQ=255)               aaGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGa                                                                                                                        <  1:490315/140‑1 (MQ=255)                aGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGa                                                                                                                        >  1:122012/1‑139 (MQ=255)                aGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGa                                                                                                                        <  2:122012/139‑1 (MQ=255)                     aGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTc                                                                                                                  >  1:204244/1‑140 (MQ=255)                       gtgtTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCTCCTGTTACAACCCAGAAAGTTCCg                                                                                                                <  1:161716/140‑1 (MQ=255)                        tgtTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCgg                                                                                                               <  2:199714/140‑1 (MQ=255)                          ttCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAg                                                                                                             <  1:166087/140‑1 (MQ=255)                            cAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGt                                                                                                           <  2:85760/140‑1 (MQ=255)                                  tttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACata                                                                                                     >  2:349570/1‑140 (MQ=255)                                  tttATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACata                                                                                                     >  1:292958/1‑140 (MQ=255)                                    tATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTGCCGGAGGTGACATATg                                                                                                   <  2:487709/140‑1 (MQ=255)                                            aaGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCaa                                                                                           <  1:214906/140‑1 (MQ=255)                                              gTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCaaaa                                                                                         >  1:526959/1‑140 (MQ=255)                                                 ttgttgAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACg                                                                                      >  1:52094/1‑140 (MQ=255)                                                      gAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCg                                                                                 <  2:292958/140‑1 (MQ=255)                                                         aTAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTa                                                                              >  1:84601/1‑140 (MQ=255)                                                         aTAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTa                                                                              >  1:320096/1‑140 (MQ=255)                                                                  gTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAAc                                                                     >  2:386924/1‑140 (MQ=255)                                                                        gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCg                                                                                 >  2:351144/1‑122 (MQ=255)                                                                        gtgATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCg                                                                                 <  1:351144/122‑1 (MQ=255)                                                                                   cGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTggg                                                    >  2:541055/1‑140 (MQ=255)                                                                                     cacaTAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTc                                                  >  1:587802/1‑140 (MQ=255)                                                                                            cccACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTggtggt                                           <  1:386924/140‑1 (MQ=255)                                                                                             ccACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTa                                          <  1:541055/140‑1 (MQ=255)                                                                                                   ggTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAAtggtg                                    >  1:53664/1‑140 (MQ=255)                                                                                                     tgttgtCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                  <  1:555050/140‑1 (MQ=255)                                                                                                     tgttgtCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATggtggt                                  <  2:52094/140‑1 (MQ=255)                                                                                                        tgtCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCgg                               >  1:3741/1‑140 (MQ=255)                                                                                                         gtCTATACTTTACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGc                              <  2:204244/140‑1 (MQ=255)                                                                                                                   tACACATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTAtt                    >  2:605909/1‑140 (MQ=255)                                                                                                                    acacATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTc                   >  1:455648/1‑140 (MQ=255)                                                                                                                    acacATAAGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTc                   >  1:139811/1‑140 (MQ=255)                                                                                                                          aaGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCt             <  2:526959/140‑1 (MQ=255)                                                                                                                          aaGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCt             >  1:467787/1‑140 (MQ=255)                                                                                                                           aGGAAGAGGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTc            <  2:455648/140‑1 (MQ=255)                                                                                                                                 agGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAAcc        >  2:114360/1‑138 (MQ=255)                                                                                                                                 agGGGTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAAcc        <  1:114360/138‑1 (MQ=255)                                                                                                                                     gTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAc  <  2:3741/140‑1 (MQ=255)                                                                                                                                     gTATCCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAc  <  2:320096/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AGTGGACTATTAGAAAGTCAAGGTGTTCAGGCGTTTATTTGTAAAGTTTTGTTGAAATAAGGGTTGTAATTGTGATCACGCCCGCACATAACCCACTGGGTGTTGTCTATACTTTACACATAAGGAAGAGGGGTATTCCCTGTTACAACCCAGAAAGTTCCGGAGGTGACATATGAAACGCAAAAACGCTTCGTTACTCGGTAACGTGCTCATGGGGTTGGGTCTGGTGGTAATGGTGGTCGGCGTGGGGTATTCAATCCTCAACCAGTTAC  >  NC_000913/1258619‑1258890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑