Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,390,618	A→G	intergenic (‑20/+62)	mpaA ← / ← ymjC	murein tripeptide amidase A/putative uncharacterized protein YmjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,390,618	0	A	G	100.0%	94.8 / NA	27	intergenic (‑20/+62)	mpaA/ymjC	murein tripeptide amidase A/putative uncharacterized protein YmjC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/15);  total (12/15)

CTGGCTGCAGGGGCAGGAAACCAGATTAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAGGGTTTGTGGATTTTT  >  NC_000913/1390486‑1390752                                                                                                                                     |                                                                                                                                       ctggctgCAGGGGCAGGAAACCAGATTAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTAc                                                                                                                                 >  2:575171/1‑140 (MQ=255)      tgCAGGGGCAGGAAACCAGATTAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCa                                                                                                                            <  2:246799/140‑1 (MQ=255)              cAGGAAACCAGATTAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTtgtg                                                                                                                    >  1:239208/1‑140 (MQ=255)                           tAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGa                                                                                                       <  1:575171/140‑1 (MQ=255)                            aaCGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGAt                                                                                                      >  2:555386/1‑140 (MQ=255)                                   cgcCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCaaa                                                                                               >  2:320221/1‑140 (MQ=255)                                              gAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTttcttc                                                                                    <  1:555386/140‑1 (MQ=255)                                                aCGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCaa                                                                                  >  1:101607/1‑140 (MQ=255)                                                  gTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACg                                                                                <  2:342965/140‑1 (MQ=255)                                                    ccGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCt                                                                              <  1:320221/140‑1 (MQ=255)                                                    ccGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCt                                                                              <  1:598173/140‑1 (MQ=255)                                                     cGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTttctt                                                                                     >  1:133442/1‑132 (MQ=255)                                                     cGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTttctt                                                                                     <  2:133442/132‑1 (MQ=255)                                                           gttctgttcCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCAt                                                                       <  1:517876/140‑1 (MQ=255)                                                              ctgttcCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGa                                                                    >  2:191618/1‑140 (MQ=255)                                                              ctgttcCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGa                                                                    >  1:340203/1‑140 (MQ=255)                                                              ctgttcCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGa                                                                    <  1:200853/140‑1 (MQ=255)                                                                  tcCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCtt                                                                >  1:294633/1‑140 (MQ=255)                                                                                                  ggCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAg                                           >  2:284840/1‑129 (MQ=255)                                                                                                  ggCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAg                                           <  1:284840/129‑1 (MQ=255)                                                                                                      ggTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTttat                            <  2:592859/140‑1 (MQ=255)                                                                                                      ggTTACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTttat                            <  2:101607/140‑1 (MQ=255)                                                                                                        ttACGGTCATGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTttatta                          <  2:87078/140‑1 (MQ=255)                                                                                                                 tGATCGTCTCTGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAg                 <  2:294633/140‑1 (MQ=255)                                                                                                                        ctctGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAGGGTTtgt          >  2:322134/1‑140 (MQ=255)                                                                                                                          ctGGTTCAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAGGGTTTGTgg        >  1:58546/1‑140 (MQ=255)                                                                                                                                cAGGAGGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAGGGTTTGTGGAttttt  <  2:58546/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CTGGCTGCAGGGGCAGGAAACCAGATTAACGGCGCGCCCAATAATGAACGTCCGTAATGTTCTGTTCCGGGCGGAAATGCGCCGCGTTCGGCGCGTGGGCGGGTTACGGTCATGATCGTCTCTGGTTCAGGAAGTATTACAGGCAGTGTTGTGCAAATTTTGCGGATTATCAAATCCTTTTCTTCAACGCTATGTCATCGATCTTGTATCACTTTTTTGCCGGAAGTTGTTTGCATTTTATTAGTTGGATAGGGTTTGTGGATTTTT  >  NC_000913/1390486‑1390752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑