Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,398,183	T→C	D147G (GAC→GGC)	uspE ←	universal stress protein with a role cellular motility

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,398,183	0	T	C	100.0%	124.4 / NA	35	D147G (GAC→GGC)	uspE	universal stress protein with a role cellular motility
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

GTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCC  >  NC_000913/1398059‑1398317                                                                                                                             |                                                                                                                                       gTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCcaca                                                                                                                         >  1:152809/1‑140 (MQ=255)  ttGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCacac                                                                                                                        <  1:298436/140‑1 (MQ=255)       ttGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCacac                                                                                                                        <  2:444554/135‑1 (MQ=255)       ttGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCacac                                                                                                                        >  1:444554/1‑135 (MQ=255)            cTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCAt                                                                                                                              <  1:137976/124‑1 (MQ=255)            cTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCAt                                                                                                                              >  2:137976/1‑124 (MQ=255)               ccAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCAt                                                                                                           <  2:288999/140‑1 (MQ=255)                   ttCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGcctgtct                                                                                                       >  1:113561/1‑134 (MQ=255)                                ttGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCa                                                                                          <  2:4921/140‑1 (MQ=255)                                                  aGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAAt                                                                        >  2:347694/1‑140 (MQ=255)                                                  aGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAAt                                                                        >  1:131513/1‑140 (MQ=255)                                                    cgcATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCa                                                                      >  2:354015/1‑140 (MQ=255)                                                                 ggCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACg                                                         >  2:60911/1‑140 (MQ=255)                                                                 ggCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACg                                                         >  2:610826/1‑140 (MQ=255)                                                                 ggCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACg                                                         >  2:302715/1‑140 (MQ=255)                                                                   ctctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCTCTTCCAGACGGt                                                       >  1:569011/1‑140 (MQ=255)                                                                   ctctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGt                                                       >  1:304024/1‑140 (MQ=255)                                                                   ctctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGt                                                       >  2:396290/1‑140 (MQ=255)                                                                     ctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCa                                                     >  1:519075/1‑140 (MQ=255)                                                                     ctTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCa                                                     >  1:605630/1‑140 (MQ=255)                                                                      ttCACCGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCAt                                                    >  2:346759/1‑140 (MQ=255)                                                                          cTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTg                                                <  1:354015/140‑1 (MQ=255)                                                                            ggCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGt                                              <  1:347694/140‑1 (MQ=255)                                                                            ggCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGt                                              <  2:378508/140‑1 (MQ=255)                                                                                       ccGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCAttttt                                   >  2:483612/1‑140 (MQ=255)                                                                                            accaGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCAc                              >  1:121334/1‑140 (MQ=255)                                                                                               aGCGCCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCaa                           <  2:121334/140‑1 (MQ=255)                                                                                                  gcCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATc                        <  1:396290/140‑1 (MQ=255)                                                                                                  gcCTTGCCTCCTTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATc                        <  1:346759/140‑1 (MQ=255)                                                                                                            ctTCCGGCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTccgccg              <  2:131513/140‑1 (MQ=255)                                                                                                                 ggCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGAt         <  2:215202/140‑1 (MQ=255)                                                                                                                 ggCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGAt         <  1:180655/140‑1 (MQ=255)                                                                                                                  gCCACGGCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATc        >  2:200634/1‑140 (MQ=255)                                                                                                                       ggCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTc   <  2:152809/140‑1 (MQ=255)                                                                                                                        gCTGGCCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTcc  <  2:605630/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                       GTTGACTTGTTCTGCCAGTTCGATCGTCTCTTTGACCAGTTTTTCATTGAGCGCATTATGATACGGCTCTTCACTGGCGAGATTCACCGCCACCAGCGCCTTGCCTCCTTCCGGCCACGGCTGGTCTTTCACCATCCACACCGGGCTTGGGCATTTGCGTAACAGATGCCAGTCCGTTGGCGTAAAAATCACCGCTTCCAGACGGTCATGTTGGTGCGCCATTTTTAGCACCAAATCGTGTCCGCCGCTGATCACTTCC  >  NC_000913/1398059‑1398317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑