BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,878	G→A	L199L (CTG→TTG)	mipA ←	scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,878	0	G	A	100.0%	116.4 / NA	34	L199L (CTG→TTG)	mipA	scaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/15); total (19/15)

ATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCA  >  NC_000913/1865742‑1866012

aTCCCGGTAGAAATCGGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTa                                                                                                                                     <  2:447799/140‑1 (MQ=255)

      gTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCt                                                                                                                               >  2:508191/1‑140 (MQ=255)

            aTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCt                                                                                                                         >  1:317191/1‑140 (MQ=255)

            aTCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCt                                                                                                                         >  1:581748/1‑140 (MQ=255)

                  ccAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt                                                                                                                   <  2:317191/140‑1 (MQ=255)

                  ccAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGtt                                                                                                                   <  2:581748/140‑1 (MQ=255)

                     gTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGCTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCgg                                                                                                                >  1:334282/1‑140 (MQ=255)

                                aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCAc                                                                                                     >  2:83928/1‑140 (MQ=255)

                                aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCAc                                                                                                     >  2:261634/1‑140 (MQ=255)

                                aTCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCAc                                                                                                     >  2:398682/1‑140 (MQ=255)

                                               gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAg                                                                                      <  2:154492/140‑1 (MQ=255)

                                                 cAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCg                                                                                    <  1:398682/140‑1 (MQ=255)

                                                 cAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCg                                                                                    <  1:83928/140‑1 (MQ=255)

                                                 cAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCg                                                                                    <  1:261634/140‑1 (MQ=255)

                                                     aaCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGAct                                                                                <  1:443995/140‑1 (MQ=255)

                                                          catcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCg                                                                                        <  1:98642/127‑1 (MQ=255)

                                                          catcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCg                                                                                        >  2:98642/1‑127 (MQ=255)

                                                          catcaGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTg                                                                           >  2:94012/1‑140 (MQ=255)

                                                                    acGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGc                                                                 >  2:518519/1‑140 (MQ=255)

                                                                                  cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCg                                                   >  2:250392/1‑140 (MQ=255)

                                                                                               acTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGCTTTCGc                                      >  1:377985/1‑140 (MQ=255)

                                                                                                 tCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTg                                    >  2:446473/1‑140 (MQ=255)

                                                                                                  ccAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGCGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGt                                   >  2:275382/1‑140 (MQ=255)

                                                                                                      tCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCa                               <  2:362391/140‑1 (MQ=255)

                                                                                                         ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTg                            >  2:266112/1‑140 (MQ=255)

                                                                                                         ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTg                            <  1:508191/140‑1 (MQ=255)

                                                                                                          cGAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                           <  1:446473/140‑1 (MQ=255)

                                                                                                           gAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGca                          >  2:122179/1‑140 (MQ=255)

                                                                                                           gAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGca                          <  1:486545/140‑1 (MQ=255)

                                                                                                           gAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGca                          >  1:365130/1‑140 (MQ=255)

                                                                                                           gAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                           <  2:17487/139‑1 (MQ=255)

                                                                                                           gAGGAAGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGc                           >  1:17487/1‑139 (MQ=255)

                                                                                                                aGTTGTAGCTGGCGCTCAGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCa                     <  1:122179/140‑1 (MQ=255)

                                                                                                                                 aGCTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGt    >  2:497051/1‑140 (MQ=255)

                                                                                                                                   cTCCAAGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCa  >  2:493325/1‑140 (MQ=255)

ATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCA  >  NC_000913/1865742‑1866012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`