Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,260,472	A→G	G313G (GGT→GGC)	fruA ←	fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,472	0	A	G	100.0%	96.1 / NA	29	G313G (GGT→GGC)	fruA	fructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/10);  total (19/10)

TGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCA  >  NC_000913/2260342‑2260605                                                                                                                                   |                                                                                                                                      tGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTg                                                                                                                              <  2:133127/140‑1 (MQ=255) tGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTg                                                                                                                              <  2:42061/140‑1 (MQ=255)   gAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGAc                                                                                                                            <  1:88418/140‑1 (MQ=255)           cGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCa                                                                                                                    <  2:317036/140‑1 (MQ=255)             gAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATa                                                                                                                  >  2:592704/1‑140 (MQ=255)                     aGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGGAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAAt                                                                                                          <  2:319979/140‑1 (MQ=255)                            gTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGAccc                                                                                                   >  2:252338/1‑140 (MQ=255)                                 aGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGt                                                                                              <  2:312010/140‑1 (MQ=255)                                                tgtgGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATc                                                                               >  1:111447/1‑140 (MQ=255)                                                tgtgGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATc                                                                               >  1:91419/1‑140 (MQ=255)                                                    gCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATGCCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCa                                                                           <  1:548434/140‑1 (MQ=255)                                                          tCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGaa                                                                     >  1:72939/1‑140 (MQ=255)                                                            aaTTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGaaaa                                                                   <  2:342268/140‑1 (MQ=255)                                                             atggCGTATTGTTTAACTCCTCAATGTAACCACCCAGGAAGCCGCCAAAAATACCACCAAAAAAGCCAGGCCGGTTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAg                                                                  <  2:588540/137‑1 (MQ=255)                                                                   tACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGACAata                                                            >  2:512335/1‑140 (MQ=255)                                                                    aCTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATCCCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGa                                                                      <  1:149602/129‑1 (MQ=255)                                                                    aCTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGa                                                                      >  2:149602/1‑129 (MQ=255)                                                                                cGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTa                                               >  1:81789/1‑140 (MQ=255)                                                                                cGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTa                                               <  1:252338/140‑1 (MQ=255)                                                                                cGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTa                                               >  1:16008/1‑140 (MQ=255)                                                                                  cAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTAcc                                             >  2:454982/1‑140 (MQ=255)                                                                                     tGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGc                                          <  2:72939/140‑1 (MQ=255)                                                                                     tGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGc                                          >  1:317951/1‑140 (MQ=255)                                                                                       tAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCAc                                        >  1:239893/1‑140 (MQ=255)                                                                                        aaCCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCAcc                                       >  1:545385/1‑140 (MQ=255)                                                                                             gccagGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAg                                  >  2:556273/1‑140 (MQ=255)                                                                                               cagGAAGCCCGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAgcg                                >  1:505468/1‑140 (MQ=255)                                                                                                 ggAAGCCCGCAATAATCCCACAAATGAAGCCAGGGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCa                              <  1:306166/140‑1 (MQ=255)                                                                                                       ccGCAATAATCCCCCCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCt                        <  1:123822/140‑1 (MQ=255)                                                                                                        cGCAATAATACCACCAATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTg                       >  1:578868/1‑140 (MQ=255)                                                                                                                     ccaATGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCTCAAAGGCTGAACCACCACCAAt          >  1:435491/1‑140 (MQ=255)                                                                                                                         tGAAGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGc      >  2:116283/1‑140 (MQ=255)                                                                                                                            aGCCAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATc   >  1:544227/1‑140 (MQ=255)                                                                                                                             cccAGAGCCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCa  <  1:454982/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      TGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGGCCCGGACGATCGGCAATGGAAAAGGCAATATAACCTGCCAGTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCA  >  NC_000913/2260342‑2260605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑