Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,346,909	T→C	V682A (GTG→GCG)	nrdA →	ribonucleoside‑diphosphate reductase 1 subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,346,909	0	T	C	100.0%	117.4 / NA	33	V682A (GTG→GCG)	nrdA	ribonucleoside‑diphosphate reductase 1 subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/16);  total (17/16)

GCGGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCA  >  NC_000913/2346780‑2347043                                                                                                                                  |                                                                                                                                       gcgGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATg                                                                                                                              <  2:62291/140‑1 (MQ=255) gcgGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATg                                                                                                                              <  2:58930/140‑1 (MQ=255)            aGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTAt                                                                                                                   <  1:45637/140‑1 (MQ=255)             gCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATc                                                                                                                  >  2:48020/1‑140 (MQ=255)                    aGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGAt                                                                                                               <  2:332413/136‑1 (MQ=255)                    aGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGAt                                                                                                               >  1:332413/1‑136 (MQ=255)                               aCGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGcc                                                                                                <  2:46246/140‑1 (MQ=255)                                          cgcCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGcc                                                                                                <  2:478757/129‑1 (MQ=255)                                          cgcCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGGATCATGCAGAAATTTATCGATCAGTCGATCTCTGcc                                                                                                >  1:478757/1‑129 (MQ=255)                                             cAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGa                                                                                  >  1:301477/1‑140 (MQ=255)                                              aGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGAt                                                                                 <  1:48020/140‑1 (MQ=255)                                                    tgCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCa                                                                           <  2:440506/140‑1 (MQ=255)                                                         gACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCtt                                                                      >  1:286233/1‑140 (MQ=255)                                                          aCTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTc                                                                     <  2:514614/140‑1 (MQ=255)                                                                    ccTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGGAAATTAACGATCAGTTCATTTCTGCCCGCACCAACTTCCATCAGTCACGCTTCCCCTTAggca                                                           >  2:564259/1‑138 (MQ=255)                                                                                  aTGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATgcag                                             <  2:301477/140‑1 (MQ=255)                                                                                   tGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGTCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATgcagc                                            >  1:65569/1‑140 (MQ=255)                                                                                     aGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGGATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGcagcag                                          >  2:31738/1‑140 (MQ=255)                                                                                       ctgctgTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGtt                                        <  2:286233/140‑1 (MQ=255)                                                                                       ctgctgTGGGAAATGCAGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGtt                                        <  2:552584/140‑1 (MQ=255)                                                                                          ctgTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCt                                     >  1:201542/1‑140 (MQ=255)                                                                                          ctgTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCt                                     >  1:415094/1‑140 (MQ=255)                                                                                             tgGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGaa                                  >  2:16191/1‑140 (MQ=255)                                                                                               ggAAATGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAg                                >  1:292822/1‑140 (MQ=255)                                                                                                  aaTGCCGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGAcc                             <  1:14788/140‑1 (MQ=255)                                                                                                    tGCCGGGTAACGATGGTTATCTGCAACTGGCGGGGATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTg                           >  1:67263/1‑140 (MQ=255)                                                                                                       cGGGTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTc                        >  1:91684/1‑140 (MQ=255)                                                                                                         ggTAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCAc                      >  1:513681/1‑140 (MQ=255)                                                                                                           tAACGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCg                    <  1:526363/140‑1 (MQ=255)                                                                                                            aaCGATGGTTATCTGCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGTCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGc                   <  2:65569/140‑1 (MQ=255)                                                                                                                          gCAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCGGGAAAAGTGCCGATGCGGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCgggg     >  2:494363/1‑140 (MQ=255)                                                                                                                           cAACTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGt    <  2:67263/140‑1 (MQ=255)                                                                                                                             aCTGGCGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCa  >  2:368252/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                       GCGGTTACGTCAGCATCAAAGCGTCGAAAGACGGTATTTTGCGCCAGGTGGTGCCGGACTACGAGCACCTGCACGACGCCTATGAGCTGCTGTGGGAAATGCCGGGTAACGATGGTTATCTGCAACTGGTGGGTATCATGCAGAAATTTATCGATCAGTCGATCTCTGCCAACACCAACTACGATCCGTCACGCTTCCCGTCAGGAAAAGTGCCGATGCAGCAGTTGCTGAAAGACCTGCTCACCGCCTACAAATTCGGGGTCA  >  NC_000913/2346780‑2347043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑