Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,531,168	T→C	V22V (GTA→GTG)	zipA ←	cell division protein ZipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,531,168	0	T	C	100.0%	114.3 / NA	35	V22V (GTA→GTG)	zipA	cell division protein ZipA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/21);  total (14/21)

ACCTCACCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGTACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCAAACATTTACTCACTAAGAGTATTTGCCGAT  >  NC_000913/2531037‑2531301                                                                                                                                    |                                                                                                                                      aCCTCACCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCCGAAACCACGCACCAGTaa                                                                                                                               >  1:162821/1‑140 (MQ=255)   cTCACCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAg                                                                                                                             <  2:571950/140‑1 (MQ=255)    tCACCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGc                                                                                                                            >  2:96126/1‑140 (MQ=255)      aCCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGa                                                                                                                          >  2:68681/1‑140 (MQ=255)       ccAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGAt                                                                                                                         <  1:494304/140‑1 (MQ=255)        cAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTCTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGAtt                                                                                                                        >  2:568654/1‑140 (MQ=255)                tcatcaTCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGAt                                                                                                                <  1:96126/140‑1 (MQ=255)                tcatcaTCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGAt                                                                                                                <  1:568654/140‑1 (MQ=255)                  atcatcTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATcg                                                                                                              >  2:596284/1‑140 (MQ=255)                          cGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCcaaca                                                                                                      <  1:68681/140‑1 (MQ=255)                           gACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCcaacaa                                                                                                     <  1:596284/140‑1 (MQ=255)                               tCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGAt                                                                                                 <  1:513806/140‑1 (MQ=255)                                ccTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGAtt                                                                                                >  1:205826/1‑140 (MQ=255)                                    gTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCcaa                                                                                                        <  2:604690/128‑1 (MQ=255)                                    gTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCcaa                                                                                                        >  1:604690/1‑128 (MQ=255)                                            aaTCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGc                                                                                    >  1:12833/1‑140 (MQ=255)                                              tcgtcgTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGGAAAGCGATTATTGCGATCGCGCCCACAATGATTAATATCAAACGCaa                                                                                  >  2:228956/1‑140 (MQ=255)                                               cgtcgtCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCaaa                                                                                 >  1:9441/1‑140 (MQ=255)                                                  cgtcACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATcc                                                                              <  2:82848/140‑1 (MQ=255)                                                   gtcACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCt                                                                             >  1:368627/1‑140 (MQ=255)                                                   gtcACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCt                                                                             >  2:90419/1‑140 (MQ=255)                                                      aCGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGca                                                                          <  1:90419/140‑1 (MQ=255)                                                      aCGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGACCAGAAACCATGCCCCAGTAAAGCGATTATGGCGAACGCGCCAACAAAGATTAATATCAGACGCCAAACCTGcc                                                                          >  2:217971/1‑139 (MQ=255)                                                           ttGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCAtt                                                                     <  2:9441/140‑1 (MQ=255)                                                                ttCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATAtt                                                                <  2:368627/140‑1 (MQ=255)                                                                ttCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATAtt                                                                >  2:466519/1‑140 (MQ=255)                                                                        ttttAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATTCTGCATCATTATATTCTCtgt                                                          <  1:430377/138‑1 (MQ=255)                                                                        ttttAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATTCTGCATCATTATATTCTCtgt                                                          >  2:430377/1‑138 (MQ=255)                                                                        ttttAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCtgttg                                                        <  1:466519/140‑1 (MQ=255)                                                                              tGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTaa                                                  <  1:228956/140‑1 (MQ=255)                                                                                      cgcgGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGc                                          <  1:217971/140‑1 (MQ=255)                                                                                               tAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGc                                 <  1:342142/140‑1 (MQ=255)                                                                                                agaagaTCGTTCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCa                                <  2:12833/140‑1 (MQ=255)                                                                                                         ttCTTTACGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCAAACATTTAc                       >  2:332358/1‑140 (MQ=255)                                                                                                                cGGCTGGTCCAGAAACCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCAAACATTTACTCACTaa                <  1:332358/140‑1 (MQ=255)                                                                                                                            aaaCCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCAAACATTTACTCACTAAGAGTATTTGCCg    <  2:84734/140‑1 (MQ=255)                                                                                                                              aCCATGCACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAATACCTTGCCACCACGGCAAACATTTACTCACTAAGAGTATTTGCCGAt  <  2:162821/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ACCTCACCAACGCCCTCATCATCTTCGACATCCTCGTCATAAGAATCGTCGTCACGTTTTGACTTCATTCGTTTTAATGGCCGATCGCGGAACATAGAAGATCGTTCTTTACGGCTGGTCCAGAAACCATGTACCAGTAAAGCGATTATGGCGATCGCGCCAACAATGATTAATATCAGACGCAAATCCTGCATCATTATATTCTCTGTTGTTCTAACACCTTGCCACCACGGCAAACATTTACTCACTAAGAGTATTTGCCGAT  >  NC_000913/2531037‑2531301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑