Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,535	T→C	E167G (GAG→GGG)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,535	0	T	C	100.0%	22.9 / NA	8	E167G (GAG→GGG)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/1);  total (7/1)

TTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCC  >  NC_000913/2734424‑2734659                                                                                                                  |                                                                                                                              ttCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCCCGAA‑‑CCCCCCCCCCCACTTCGAACGCGCGTGGAcc                                                                                                   <  1:12403/140‑1 (MQ=255)     ccAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCCCCCCCCCCCCTTCGACCCCCCGGGAACCAAtt                                                                                               >  2:357791/1‑140 (MQ=255)                         tGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCCCCCCCCCCCCTTCGAACCCGCGTGGCCCAATCCCCGCCCCAACCCAGGCgaa                                                                           >  2:216804/1‑139 (MQ=255)                                         gATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCCCCCCCCCCCCTTCCAAAACCCGGGGGCCAAATGCCCGGCCCAACCAGGCCGGAAAA‑TTTTCCCGAttt                                                          >  2:155413/1‑139 (MQ=255)                                                      aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAAC‑CCCCCCCCCCCCCTCCGAACCCCGCTGGACCAATTCCCGGCCC‑AACCCGGCCAGAAAATTTTTCCGGATTTTCACAAAAAcgg                                              >  2:613408/1‑138 (MQ=255)                                                      aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAgg                                             >  2:585358/1‑140 (MQ=255)                                                                        ccTTGCTTTAGCGTCTACTGCCAAAAACCCCCCCCGAA‑‑CCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                           <  1:76246/139‑1 (MQ=255)                                                                          ttGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAAC‑CCCCCCCCCCCACTCCGAACCCGCGCGGCCCAATTGCCGCCCCTACCCCGGCGGGAATA‑TTTTCCGGGTTATCAGCAAAACCGACACCCGTCTCTTCCAacac                          >  2:195684/1‑140 (MQ=255)                                                                            tcTTTAGCGTCTACTTCCAAAAACCCCCCCCGAAC‑CCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGc                        <  1:613408/139‑1 (MQ=255)                                                                                    gTCTACTGCCAAAAACCCCCCCCGAACCCCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                 <  1:297329/140‑1 (MQ=255)                                                                                           gCCATAAACGCCCCGCGAA‑‑CCCCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACg        <  1:357791/140‑1 (MQ=255)                                                                                                 aaCGCCTCGCGAA‑‑CCCCCCCCCCCACTTCGAACCCCCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTTTCAGCAAAACAGGAAACCGTCCCTTCCAGCCCCCCTGCCCACAGTCCACgccagcc  >  2:466668/1‑140 (MQ=255)                                                                                                                  |                                                                                                                              TTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGCGAA‑‑CCTCCCCCCCCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATA‑TTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCC  >  NC_000913/2734424‑2734659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑