Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,516	G→A	V185V (GTG→GTA)	ypjD →	cytochrome c assembly family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,516	0	G	A	100.0%	122.8 / NA	35	V185V (GTG→GTA)	ypjD	cytochrome c assembly family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

CGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACATTAT  >  NC_000913/2748384‑2748645                                                                                                                                     |                                                                                                                                  cgccCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTaa                                                                                                                            <  2:343126/140‑1 (MQ=255)   cccTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACg                                                                                                                          >  1:148301/1‑140 (MQ=255)    ccTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGc                                                                                                                         <  2:148301/140‑1 (MQ=255)           gcgcTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCt                                                                                                                  >  2:327775/1‑140 (MQ=255)              cTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCt                                                                                                                  <  2:589905/137‑1 (MQ=255)              cTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCt                                                                                                                  >  1:589905/1‑137 (MQ=255)              cTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTg                                                                                                               >  2:417552/1‑140 (MQ=255)                  aaCTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACt                                                                                                           >  1:258808/1‑140 (MQ=255)                     tGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGc                                                                                                        <  1:48310/140‑1 (MQ=255)                     tGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGc                                                                                                        <  2:258808/140‑1 (MQ=255)                       gCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCa                                                                                                             >  2:135613/1‑133 (MQ=255)                       gCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCa                                                                                                             <  1:135613/133‑1 (MQ=255)                       gCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCt                                                                                                      <  2:205026/140‑1 (MQ=255)                                 attACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCa                                                                                                             <  2:464586/123‑1 (MQ=255)                                 attACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCa                                                                                                             >  1:464586/1‑123 (MQ=255)                                 attACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATg                                                                                            >  2:331923/1‑140 (MQ=255)                                    aCCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGcac                                                                                         <  1:417552/140‑1 (MQ=255)                                       aaCTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGTTGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAAc                                                                                      >  1:250399/1‑140 (MQ=255)                                       aaCTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAAc                                                                                      <  1:548666/140‑1 (MQ=255)                                              gaaCAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTa                                                                                                <  1:184298/123‑1 (MQ=255)                                              gaaCAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTa                                                                                                >  2:184298/1‑123 (MQ=255)                                              gaaCAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTa                                                                               <  1:365803/140‑1 (MQ=255)                                                    gaagCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATgg                                                                         >  2:84548/1‑140 (MQ=255)                                                            cGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATc                                                                 >  1:225101/1‑140 (MQ=255)                                                                     aGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCt                                                        <  1:173601/140‑1 (MQ=255)                                                                       gAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCtgt                                                      <  1:84548/140‑1 (MQ=255)                                                                            gccgccATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCtgt                                                      >  1:549060/1‑135 (MQ=255)                                                                            gccgccATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCtgt                                                      <  2:549060/135‑1 (MQ=255)                                                                                        gaGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgt                                     <  1:419708/140‑1 (MQ=255)                                                                                          gTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgtgg                                   <  1:327775/140‑1 (MQ=255)                                                                                          gTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgtgg                                   <  2:69235/140‑1 (MQ=255)                                                                                              cGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgtgg                                   <  2:313924/136‑1 (MQ=255)                                                                                              cGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgtgg                                   >  1:313924/1‑136 (MQ=255)                                                                                                 gCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCt                            <  1:331923/140‑1 (MQ=255)                                                                                                                           ttGGCGTGGTACTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACATTAt  >  1:569009/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  CGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACATTAT  >  NC_000913/2748384‑2748645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑