| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 375,075 | G→A | intergenic (+194/‑384) | mhpE → / → mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 375,075 | 0 | G | A | 96.8% | 85.7 / ‑3.9 | 31 | intergenic (+194/‑384) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (19/11); total (20/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
GGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGA > NC_000913/374939‑375197 | GGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGaa < 2:478779‑M1/140‑1 (MQ=255) CGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAAtt < 2:469758‑M1/140‑1 (MQ=255) TGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTg > 1:212602‑M1/1‑140 (MQ=255) ATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGAt > 2:278685‑M1/1‑140 (MQ=255) CCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATaa > 1:304901‑M1/1‑140 (MQ=255) CCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATaa > 2:456313‑M1/1‑140 (MQ=255) CCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATaa > 1:478779‑M1/1‑140 (MQ=255) CGGCATACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAg > 1:250100‑M1/1‑140 (MQ=255) GCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGAc > 2:601470‑M1/1‑140 (MQ=255) GCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGAc < 2:241518‑M1/140‑1 (MQ=255) TACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGAcgcg > 2:175655‑M1/1‑140 (MQ=255) ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt > 1:557142/1‑140 (MQ=38) ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACCCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt > 2:281537/1‑140 (MQ=255) gCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTc > 2:242367/1‑140 (MQ=37) gagaATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCAt > 2:260537/1‑140 (MQ=21) tGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt < 2:557142/140‑1 (MQ=17) aGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTg > 1:366785/1‑140 (MQ=18) gTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTgcg < 1:320273/140‑1 (MQ=14) tCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCAt < 2:134159/127‑1 (MQ=11) tCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCAt > 1:134159/1‑127 (MQ=11) cTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATcc > 1:93583/1‑140 (MQ=17) aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtcta > 2:244995‑M1/1‑136 (MQ=255) aCGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGCAAAGCGTCGCATCAGACAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtcta > 2:180627‑M1/1‑136 (MQ=255) gACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctaca > 1:529098‑M1/1‑134 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgc < 1:244995‑M1/140‑13 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCGCAAAGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgcc < 1:161108‑M1/140‑14 (MQ=255) tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCTGGATGCGGCGTAAACGCCTTATCCGGcctacacttcgcccgt > 1:77207‑M1/1‑124 (MQ=255) cgTGAACGCCTTATCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgcccataaaccg > 1:337828‑M1/1‑118 (MQ=255) ccGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgcccataaaccgtaggcctgataag < 1:300748‑M1/140‑36 (MQ=255) ccGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgcccataaaccgtaggcctgataag < 2:529098‑M1/140‑36 (MQ=255) cGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGtctacacttcgcccataaaccgtaggcctgataagac < 2:337828‑M1/140‑38 (MQ=255) | GGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGA > NC_000913/374939‑375197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |