Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,919,458	A→G	L430P (CTG→CCG)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,919,458	0	A	G	100.0%	141.0 / NA	39	L430P (CTG→CCG)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

TGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTT  >  NC_000913/2919324‑2919594                                                                                                                                       |                                                                                                                                         tGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTAc                                                                                                                                     <  2:100811/140‑1 (MQ=255)      cGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCAt                                                                                                                                >  2:306160/1‑140 (MQ=255)        ccACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCg                                                                                                                              >  2:94593/1‑140 (MQ=255)                aTAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGcg                                                                                                                      <  2:518608/140‑1 (MQ=255)                    ccTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGcgtc                                                                                                                    <  1:309929/138‑1 (MQ=255)                    ccTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGcgtc                                                                                                                    >  2:309929/1‑138 (MQ=255)                       gCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTAc                                                                                                               <  2:503049/140‑1 (MQ=255)                        cATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGGCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTAcg                                                                                                              >  1:144078/1‑140 (MQ=255)                          tttCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTAcgcg                                                                                                            <  2:515547/140‑1 (MQ=255)                             cAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCAc                                                                                                         >  2:64162/1‑140 (MQ=255)                              aGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCAcc                                                                                                        >  1:403869/1‑140 (MQ=255)                               gTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCg                                                                                                       >  2:145233/1‑140 (MQ=255)                                  acaacaGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCg                                                                                                       >  1:330790/1‑137 (MQ=255)                                  acaacaGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCg                                                                                                       <  2:330790/137‑1 (MQ=255)                                       aGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAg                                                                                               <  1:306160/140‑1 (MQ=255)                                       aGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAg                                                                                               >  2:447096/1‑140 (MQ=255)                                        ggCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGa                                                                                              >  1:192280/1‑140 (MQ=255)                                                      agaACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTc                                                                                >  2:506410/1‑140 (MQ=255)                                                       gaACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCa                                                                               >  2:17068/1‑140 (MQ=255)                                                           tCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAtgtg                                                                           <  1:506410/140‑1 (MQ=255)                                                             atatTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGcc                                                                         <  1:17068/140‑1 (MQ=255)                                                                     gTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAtgt                                                                            <  1:431178/129‑1 (MQ=255)                                                                     gTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAtgt                                                                            >  2:431178/1‑129 (MQ=255)                                                                     gTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAt                                                                              <  1:421144/127‑1 (MQ=255)                                                                     gTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGAGCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCAt                                                                              >  2:421144/1‑127 (MQ=255)                                                                            ttCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTcc                                                          <  2:192280/140‑1 (MQ=255)                                                                            ttCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTcc                                                          <  1:64162/140‑1 (MQ=255)                                                                                      aTGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGtt                                                >  1:392488/1‑140 (MQ=255)                                                                                       tGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTc                                               <  2:270150/140‑1 (MQ=255)                                                                                             ccGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGcc                                         >  1:343156/1‑140 (MQ=255)                                                                                              cGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGccc                                        <  1:447096/140‑1 (MQ=255)                                                                                                 aaaCGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGa                                     <  1:145233/140‑1 (MQ=255)                                                                                                      ggACGTTTACGGTCAAAGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCggg                                <  2:403869/140‑1 (MQ=255)                                                                                                                     aaGGTCCAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGc                 >  2:613586/1‑140 (MQ=255)                                                                                                                           cAGCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAAtttt           >  1:494969/1‑140 (MQ=255)                                                                                                                             gCCGGGGATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTc         <  1:94593/140‑1 (MQ=255)                                                                                                                                ggggATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt      <  2:392488/140‑1 (MQ=255)                                                                                                                                ggggATCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGt      >  1:527350/1‑140 (MQ=255)                                                                                                                                    aTCGGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGttttt  <  2:494969/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TGACCCGCCACCGGGATAACCTGCATTTCAGTAACAACAGGCGTCGTAAATTGAGAACTCATATTTATGTCCTTTTTCAGAATCAATGACGGCCGAAAACGGGACGTTTACGGTCAAAGGTCCAGCCGGGGATCAGGTACTGCATCGGACCTGCGTCGTTACGCGCACCGCCAGGCAGACGTTTATAGGCCTCATGTGCCTTTTGTACCTGTTCCCAGTCCAGTTCCACGCCCAGACCGGGCGCATCAGGAACGGCAATTTTTCCGTTTTT  >  NC_000913/2919324‑2919594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑