Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,172	C→T	G29G (GGG→GGA)	kduD ←	putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,172	0	C	T	100.0%	127.8 / NA	37	G29G (GGG→GGA)	kduD	putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

CTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAAC  >  NC_000913/2983039‑2983305                                                                                                                                      |                                                                                                                                      cttgAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAAc                                                                                                                                 >  1:167737/4‑140 (MQ=255) cTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAAAGACGCCCCAGCGCTGTGACCTGCCCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAAAGTCACAGCCCGCTTGCGCCCGTCCCCAc                                                                                                                                 >  2:572641/1‑140 (MQ=255)    gAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGcc                                                                                                                              <  2:84231/140‑1 (MQ=255)    gAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGcc                                                                                                                              <  2:6026/140‑1 (MQ=255)     aaTACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCa                                                                                                                             <  2:281205/140‑1 (MQ=255)                    tCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACccagtc                                                                                                              <  1:498982/140‑1 (MQ=255)                            cggcggTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATca                                                                                                      >  2:28571/1‑140 (MQ=255)                            cggcggTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATca                                                                                                      <  2:533958/140‑1 (MQ=255)                                      gCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTga                                                                                            >  2:303622/1‑140 (MQ=255)                                      gCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTga                                                                                            <  1:212574/140‑1 (MQ=255)                                          aaaaaaCGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATGACAACCAGTgacggc                                                                                        <  2:558439/140‑3 (MQ=255)                                                cgacgCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAAc                                                                                  >  2:394743/1‑140 (MQ=255)                                                 gacgCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACt                                                                                 <  2:225314/140‑1 (MQ=255)                                                        cAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCtt                                                                          <  1:303622/140‑1 (MQ=255)                                                         aGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTc                                                                         <  2:167737/140‑1 (MQ=255)                                                          gcgcTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCg                                                                        <  1:394743/140‑1 (MQ=255)                                                            gcTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCgag                                                                      <  1:28571/140‑1 (MQ=255)                                                               gtgACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCtt                                                                          <  1:538776/133‑1 (MQ=255)                                                               gtgACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCtt                                                                          >  2:538776/1‑133 (MQ=255)                                                                tgACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGaa                                                                  >  2:573749/1‑140 (MQ=255)                                                                  aCCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGaaaa                                                                <  1:151049/140‑1 (MQ=255)                                                                              gTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCCCAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAAt                                                    <  2:516274/140‑1 (MQ=255)                                                                                 tCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCAt                                                 >  1:230392/1‑140 (MQ=255)                                                                                         ttCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTaa                                                       >  2:244826/1‑126 (MQ=255)                                                                                         ttCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTaa                                                       <  1:244826/126‑1 (MQ=255)                                                                                         ttCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATAc                                         >  2:10272/1‑140 (MQ=255)                                                                                         ttCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATAc                                         <  1:572641/140‑1 (MQ=255)                                                                                                   ttAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGtt                               >  1:450792/1‑140 (MQ=255)                                                                                                   ttAATGCCAACAATGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGtt                               >  2:96345/1‑140 (MQ=255)                                                                                                           aacaaTGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCca                       >  2:467389/1‑140 (MQ=255)                                                                                                             caaTGTCACAGCCCGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCcaca                     >  2:485168/1‑140 (MQ=255)                                                                                                                        cccGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa          >  2:573317/1‑140 (MQ=255)                                                                                                                        cccGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATG‑CCACAACTAGCGCAAAt         >  1:299155/1‑140 (MQ=255)                                                                                                                          cGCTTGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATc        >  2:538549/1‑140 (MQ=255)                                                                                                                              tGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTa    >  1:33934/1‑140 (MQ=255)                                                                                                                              tGCGCCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATG‑CCACAACTAGCGCAAATCTTTaa   <  2:299155/140‑1 (MQ=255)                                                                                                                                cgcCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAAc  >  2:180351/1‑140 (MQ=255)                                                                                                                                cgcCAGTCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAAc  >  1:180153/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CTGGAATACCATCAATCTTTCGCAGATCGGCGGTCAGGCTTAAAAAACGACGCCCCAGCGCTGTGACCTGCTCGATGGTTTCAGTCGGTTCAACGATGTTAATGCCAACAATGTCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAAC  >  NC_000913/2983039‑2983305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑