Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,582	A→G	V139A (GTG→GCG)	zapE ←	cell division factor ZapE with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,582	0	A	G	100.0%	139.4 / NA	39	V139A (GTG→GCG)	zapE	cell division factor ZapE with ATPase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

TTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCACGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCT  >  NC_000913/3379447‑3379718                                                                                                                                        |                                                                                                                                         tttCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTc                                                                                                                                      >  2:408235/1‑140 (MQ=255)              cATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTa                                                                                                                         >  1:514541/1‑140 (MQ=255)                           atTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCaa                                                                                                            <  1:408235/140‑1 (MQ=255)                                          cAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGAt                                                                                             <  2:311192/140‑1 (MQ=255)                                              gTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGt                                                                                         <  1:71241/140‑1 (MQ=255)                                                 aTACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTg                                                                                      >  1:301683/1‑140 (MQ=255)                                                 aTACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTg                                                                                      >  2:526589/1‑140 (MQ=255)                                                    ccGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGccc                                                                                   >  1:271227/1‑140 (MQ=255)                                                       cgagcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTg                                                                                      <  2:475277/134‑1 (MQ=255)                                                       cgagcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTg                                                                                      >  1:475277/1‑134 (MQ=255)                                                       cgagcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTg                                                                                >  2:375667/1‑140 (MQ=255)                                                         agcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGAt                                                                                             <  1:62385/125‑1 (MQ=255)                                                         agcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGAt                                                                                             >  2:62385/1‑125 (MQ=255)                                                         agcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGt                                                                                         >  2:229969/1‑129 (MQ=255)                                                         agcgaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGt                                                                                         <  1:229969/129‑1 (MQ=255)                                                            gaACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAgg                                                                           <  1:110096/140‑1 (MQ=255)                                                                   gCTTTCATCAGACCACCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAg                                                                    <  1:188540/140‑1 (MQ=255)                                                                    cTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGc                                                                   >  2:570456/1‑140 (MQ=255)                                                                          tcaGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCa                                                             >  1:43837/1‑140 (MQ=255)                                                                            aGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATg                                                           <  2:271227/140‑1 (MQ=255)                                                                                  ccAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCAccca                                                     >  1:507417/1‑139 (MQ=255)                                                                                  ccAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCg                                                     >  1:424976/1‑140 (MQ=255)                                                                                            tGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATaaaa                                           >  1:486313/1‑140 (MQ=255)                                                                                             ggCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAAc                                          >  1:500779/1‑140 (MQ=255)                                                                                               cATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACgg                                        >  2:82123/1‑140 (MQ=255)                                                                                                 tCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTg                                      <  2:301683/140‑1 (MQ=255)                                                                                                 tCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTg                                      >  2:506378/1‑140 (MQ=255)                                                                                                 tCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTg                                      >  2:65127/1‑140 (MQ=255)                                                                                                    gTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGaaa                                   >  2:10517/1‑140 (MQ=255)                                                                                                     tAATATCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAg                                  <  2:486313/140‑1 (MQ=255)                                                                                                         atCAGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCa                              <  2:514541/140‑1 (MQ=255)                                                                                                            aGAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGgc                           <  1:32718/140‑1 (MQ=255)                                                                                                             gAAACAAAAAATTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGgcg                          >  1:89903/1‑140 (MQ=255)                                                                                                                   aaaaaTTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGttt                    >  2:117321/1‑140 (MQ=255)                                                                                                                     aaaTTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTcc                  <  2:43837/140‑1 (MQ=255)                                                                                                                     aaaTTCGTCAAAACAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCACAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTcc                  <  2:507417/140‑1 (MQ=255)                                                                                                                               aaaCAGAGCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCgg        <  2:500779/140‑1 (MQ=255)                                                                                                                                    gagCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAACAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGc   <  2:424976/140‑1 (MQ=255)                                                                                                                                     agCGCGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCt  <  2:421191/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTTCGATAAAGTTCATCCGGCGGAATATTTGACGTCGCTACCAGGGTAATACCGCGAGCGAACAGGGCTTTCATCAGACCGCCAAGTAGCATGGCATCGGTAATATCAGAAACAAAAAATTCGTCAAAACAGAGCACGTCAGTTTCGGCTTTAAAGCGATCGGCAATAATTTCCAGCGGATCGGTCTGCCCCTGTAAGGCAGTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCT  >  NC_000913/3379447‑3379718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑