Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,603,930	A→G	V105A (GTG→GCG)	ftsY ←	signal recognition particle receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,603,930	0	A	G	100.0%	54.7 / NA	16	V105A (GTG→GCG)	ftsY	signal recognition particle receptor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/9);  total (7/9)

TCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTC  >  NC_000913/3603813‑3604063                                                                                                                      |                                                                                                                                      tcttcttcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTCCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGtt                                                                                                                         <  1:34802/132‑1 (MQ=255) tcttcttcCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTCCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGtt                                                                                                                         >  2:34802/1‑132 (MQ=255)              cTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTc                                                                                                        <  1:85874/136‑1 (MQ=255)              cTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTc                                                                                                        >  2:85874/1‑136 (MQ=255)                tCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCtg                                                                                                  >  1:450088/1‑140 (MQ=255)                    cAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCa                                                                                              <  1:583573/140‑1 (MQ=255)                                  tCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTc                                                                                >  2:302433/1‑140 (MQ=255)                                   ccGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCa                                                                               >  2:19017/1‑140 (MQ=255)                                        tCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTg                                                                          <  2:222271/140‑1 (MQ=255)                                                             gAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAAc                                                     <  1:19017/140‑1 (MQ=255)                                                             gAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAAc                                                     <  1:302433/140‑1 (MQ=255)                                                             gAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAAc                                                     <  2:314282/140‑1 (MQ=255)                                                                aCCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCa                                                                >  1:286902/1‑126 (MQ=255)                                                                aCCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCa                                                                <  2:286902/126‑1 (MQ=255)                                                                                     tCCGGCAACGGCAGCTCTTCACGTTCGATAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTc                             <  2:450088/140‑1 (MQ=255)                                                                                                                aTAGCCGCTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTc  >  1:511961/1‑140 (MQ=255)                                                                                                                      |                                                                                                                                      TCTTCTTCCGCCGCTTCGACAATCTCTACGGTTTCCGCTTCAGCCTGCCACTCTTCTGGCGAAACCGCTTCGGCGTTGACGTCTTCCGGCAACGGCAGCTCTTCACGTTCGATAGCCACTGGCTCCGGCGTTTCTTCTACGACCGGTTCCGGCTGTGCAACGACTTCCGCTTCAGGCTGCGCTTTTTCACTTTCAGCAACCTGTTCAGTGACTTCCACAACGTCGGCAGCAAAAGTTTCCGCCTCGGCTTC  >  NC_000913/3603813‑3604063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑